Extraction of Soluble Dietary Fibers from Pitaya Peel

doi:10.11983/CBB16116

Abstract

Abstract: Pitaya peel can be a good source for preparing dietary fibers, and such dietary fibers has excellent physicochemical properties. To stimulate the comprehensive utilization of pitaya, we optimized the extraction of soluble dietary fibers (SDF) from pitaya peel. The cellulase digestion method was used with single-factor experiments and the response surface method. The optimized extraction conditions were cellulase concentration of 0.54%, enzymolysis temperature at 50°C and solvent pH of 5.2. Under the optimal conditions, the extraction rate of SDF from pitaya peel was up to 19.81%, the water holding capacity was 31.25 g∙g^-1 and the swelling capacity was 29.11 mL∙g^-1.

Key words: Pitaya peel, soluble dietary fibers, cellulase, response surface method

Guichun Zhang, Yujing Liu, Yanmin Li, Ping Mu, Mingjuan Qu, Qing Li, Juhua Zhou. Extraction of Soluble Dietary Fibers from Pitaya Peel[J]. Chinese Bulletin of Botany, 2017, 52(5): 622-630.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB16116

https://www.chinbullbotany.com/EN/Y2017/V52/I5/622

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References 26

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Level	Factor
Level	Cellulase con- centration (%)	Enzymolysis tem- perature (°C)	Enzymolysis time (min)	pH
1 2 3 4 5 6	0 0.1 0.3 0.5 0.7 0.9	30 40 50 60 70 80	45 60 75 90 105 120	4.0 4.4 4.8 5.2 5.6 6.0

Level	Factor
Level	Cellulase con- centration (%)	Enzymolysis tem- perature (°C)	Enzymolysis time (min)	pH
1 2 3 4 5 6	0 0.1 0.3 0.5 0.7 0.9	30 40 50 60 70 80	45 60 75 90 105 120	4.0 4.4 4.8 5.2 5.6 6.0

Factor	Level
Factor	-1	0	1
X₁(pH) X₂(Enzymolysis temperature (°C)) X₃(Cellulase concentration (%))	4.8 40 0.3	5.2 50 0.5	5.6 60 0.7

Factor	Level
Factor	-1	0	1
X₁(pH) X₂(Enzymolysis temperature (°C)) X₃(Cellulase concentration (%))	4.8 40 0.3	5.2 50 0.5	5.6 60 0.7

Source of variance	Degree of freedom	Sum of squares	Mean square error	F value	P value
Regression model	9	46.02	5.11	315.01	<0.0001
X₁	1	1.75	1.75	107.72	<0.0001
X₂	1	0.34	0.34	20.97	0.0025
X₃ X₁X₂ X₁X₃ X₂X₃ X₁² X₂² X₃² Residual error Lack of fit Pure error Total deviation	1 1 1 1 1 1 1 7 3 4 16	0.20 6.250E-004 8.67 1.39 16.30 8.67 5.38 0.11 2.500E-005 0.11 46.13	0.20 6.250E-004 8.67 1.39 16.30 8.67 5.38 0.016 8.333E-006 0.028	12.42 0.039 534.31 85.78 1004.13 534.15 331.22 2.934E-004	0.0097 0.8500 <0.0001 <0.0001 <0.0001 <0.0001 <0.0001 1.0000