Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (5): 623-627.DOI: 10.11983/CBB14174

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Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum

Lili Liu, Shubo Lu, Jiaping Xu, Qingtian Zhang, Changyu Li*   

  1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China
  • Received:2014-09-22 Accepted:2015-03-30 Online:2015-09-01 Published:2015-10-09
  • Contact: Li Changyu
  • About author:

    ? These authors contributed equally to this paper

Abstract:

The hairy roots of Aconitum coreanum were used as explants to induce callus via differentiation in 1/2MS medium with 0.5-5 mg·L-1 6-BA and 0-0.5 mg·L-1 NAA, then regeneration plantlets were induced from the callus. A whole transgenetic plantlet was obtained. The optimal medium for inducing callus and adventitious buds was 1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3% sugar. The optimal medium for inducing roots was 1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3% sugar.