直接从人工老化的菜心干种子中提取基因组DNA用于RAPD分析

植物学报 ›› 1999, Vol. 16 ›› Issue (06): 701-704.

直接从人工老化的菜心干种子中提取基因组DNA用于RAPD分析

乔爱民傅家瑞

(中山大学生命科学与技术系　广州　510275）

收稿日期:1998-12-07 修回日期:1999-03-08 出版日期:1999-11-20 发布日期:1999-11-20
通讯作者: 乔爱民

Direct Extraction of Genomie DNA from Dry Artificially-aged Seeds of Flowering Chinese Cabbage and its Application in RAPD Analysis

QIAO Ai-Min and FU Jia-Rui

(Department of Life Sciences and Biotechnology, Zhangshan University, Guangzhou 510275)

Received:1998-12-07 Revised:1999-03-08 Online:1999-11-20 Published:1999-11-20
Contact: QIAO Ai-Min

摘要/Abstract

摘要： 利用40℃、100％朋对菜心种子进行人工加速老化处理获得了不同活力的种子批，利用平衡酚-氯仿法直接从人工老化的菜心干种子中提取基因组DNA，并对提取的基因组DNA进行了趾PD扩增。结果表明，所提取的基因组DNA量多，而且比较整齐一致。引物S208扩增所获得的基因组DNA指纹图谱上的DNA带清晰、明亮，从而表明利用本方法从人工老化菜心干种子中直接提取的基因组DNA完全可以用于RAPD分析。

Abstract: Flowering Chinese cabbage seeds of different vigor were obtained by treatment at 40℃ and 100% RH for different days. Genomic DNA was extracted with balanced phenol and chloroform directly from the dry aged seed. RAPD amplification was carded out on the extracted genomic DNA. The extracted genomic DNA samples were large in quantity and identical. DNA bands amplified by primer S208 were clear and bright. It could be concluded that genomic DNA extracted directly from the dry artificial aged seeds of flowering Chinese cabbage could be used for RAPD analysis.

乔爱民傅家瑞. 直接从人工老化的菜心干种子中提取基因组DNA用于RAPD分析. 植物学报, 1999, 16(06): 701-704.

QIAO Ai-Min and FU Jia-Rui. Direct Extraction of Genomie DNA from Dry Artificially-aged Seeds of Flowering Chinese Cabbage and its Application in RAPD Analysis. Chinese Bulletin of Botany, 1999, 16(06): 701-704.

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