植物学报 ›› 1999, Vol. 16 ›› Issue (06): 701-704.
• 研究报告 •
QIAO Ai-Min and FU Jia-Rui
Abstract: Flowering Chinese cabbage seeds of different vigor were obtained by treatment at 40℃ and 100% RH for different days. Genomic DNA was extracted with balanced phenol and chloroform directly from the dry aged seed. RAPD amplification was carded out on the extracted genomic DNA. The extracted genomic DNA samples were large in quantity and identical. DNA bands amplified by primer S208 were clear and bright. It could be concluded that genomic DNA extracted directly from the dry artificial aged seeds of flowering Chinese cabbage could be used for RAPD analysis.
乔爱民 傅家瑞. 直接从人工老化的菜心干种子中提取基因组DNA用于RAPD分析. 植物学报, 1999, 16(06): 701-704.
QIAO Ai-Min and FU Jia-Rui. Direct Extraction of Genomie DNA from Dry Artificially-aged Seeds of Flowering Chinese Cabbage and its Application in RAPD Analysis. Chinese Bulletin of Botany, 1999, 16(06): 701-704.