Abstract: A stable cell suspension culture was established with callus that was induced from the ripe pith of Lycium barbarum L. The protoplasts were cultured in KM liquid medium supplemented with 1.5 mg/L 6_BA, 0.5 mg/L NAA and 0.5 mg/L 2,4_D. The protoplasts started to divide after 3～4　days. The frequency determined at 7 day s was 50.3%, and cell colonies were formed after about 15 days. Microcalli could be observed in 3～4 weeks. The plating efficiency was 1.25%. Eight to seven days after the microcalli were transferred into the liquid proliferation medium (MS+1.5 mg/L 6_B+0.2 mg/L 2,4_D), a large amount of embryoids appeared on the calli. The embryonic calli were then moved on to a solid medium (MS+ 6-BA 0.2 mg/L) and green buds appeared. The frequency of shoot formation was 54.17%. The shoot rooted in the rooting medium (MS+NAA 0.2 mg/L) and the whole plants were transplanted into pots and grew well.