植物学报 ›› 1998, Vol. 15 ›› Issue (03): 65-68.

• 研究报告 • 上一篇    下一篇

苎麻体细胞胚胎发生研究初报

陈德富 陈喜文   

  1. (南开大学生物化学与分子生物学系 天津 300071)
  • 出版日期:1998-05-20 发布日期:1998-05-20
  • 通讯作者: 陈德富

Preliminary Study on Somatic Embryogenesis of Ramie

CHEN De-Fu and CHEN Xi-Wen   

  1. (Department of Biochemistry and Molecular Biology, Nankai University, Tianjin 300071)
  • Online:1998-05-20 Published:1998-05-20
  • Contact: CHEN De-Fu

摘要: 将苎麻“浏阳大叶绿”的子叶培养在附加0.55mg/L CPA、 0.05 mg/L BR 的CXW培养基上,可获得淡黄色或浅灰绿色的颗粒状愈伤组织。将愈伤组织转移至附加0.1 mg/L CPA、 0.05 mg/L NAA 、1.5 mg/L ZT的CXW培养基上继代;颗粒状结构非常明显。继续培养在添加2 mg/L Met, 3 g/L YE 的上述继代培养基上,可分化出一些相互独立的胚状体,继而发育成为胚状体幼苗。

Abstract: The light yellow or grey green granalous calli were induced from ranie cotyledons of "Liuyang Dayelu" on exw medium contatining 0.5 mg/L CPA, 0.05 mg/L BR. When subcultured on CXW medium tontaining 0.1 mg/L CPA,O.05 mg/L NAA, 1.5 mg/L ZT, the granulons structure became more prominant. After transfered to the stone medium adding 2 mg/L Met, 3 g/L YE, some somatic embryos were induced and then grew into embryo plantlets.