植物学报 ›› 2008, Vol. 25 ›› Issue (05): 591-596.

• 组织培养简讯 • 上一篇    下一篇

油樟悬浮培养及次生代谢产物的诱导

魏琴1*, 王丽2, 傅体华2, 周黎军1   

  1. 1宜宾学院西南特色经济植物保护与利用重点实验室, 宜宾 644000; 2四川农业大学, 雅安 625014
  • 收稿日期:2007-12-12 修回日期:2008-01-20 出版日期:2008-09-01 发布日期:2008-09-01
  • 通讯作者: 魏琴

Studies of Cell Suspension Culture and Induction of Secondary Metabolism Products in Cinnamomum longepaniculatum

Qin Wei1*, Li Wang2, Tihua Fu2, Lijun Zhou1   

  1. 1The Key Laboratory of Economic Plant Protection and Utilization in Southwest, Yibin College, Yibin 644000, China; 2 Sichuan Agricultural University, Ya'an 625014, China
  • Received:2007-12-12 Revised:2008-01-20 Online:2008-09-01 Published:2008-09-01
  • Contact: Qin Wei

摘要: 以油樟(Cinnamomum longepaniculatum)叶片为外植体在附加6-BA和NAA不同激素浓度组合的MS培养基上筛选质地疏松、生长旺盛的愈伤组织, 分别接种在MS、B5、WPM三种液体培养基中进行细胞悬浮培养, 并检测诱导产生的次生代谢产物。结果表明: 2 mg.L-1 6-BA+ 0.5 mg.L-1 NAA能诱导质地疏松、生长旺盛的愈伤组织, B5基本培养基中细胞长势最好; 愈伤组织在B5+2 mg.L-1 6-BA+ 0.5 mg.L-1 NAA中悬浮培养, 继代2次后形成均一的单细胞; 从油樟悬浮培养物中检测出50%以上的成分是苯甲醇。

Abstract: Well-growing and friable calli from leaves of Cinnamomum longepaniculatum were screened on Murashige and Skoog (MS) medium with different plant growth regulators including 6-benzyladenine (6-BA) and 1-naphthaleneacetic acid (NAA). Calli were cultured on MS medium, Gamborg medium (B5) and woody plant medium (WPM) through suspension culture, and secondary metabolism products were detected. Well-growing and friable-structured calli were induced on 2 mg.L-1 6-BA + 0.5 mg.L-1 NAA, and growth of calli was best on B5 medium in three liquid media. Single cells were formed on B5 medium with 0.2 mg.L-1 6-BA + 0.5 mg.L-1 NAA after two periods of subcultivation. More than 50% benzenemethanol was detected in the secondary metabolism products.