Abstract: Pollen is a highly reduced organism that plays a great role in plant sexual reproduction as the male gametophyte. Much research has focused on the molecular mechanism of pollen development, the interaction with the pistil, germination, and pollen tube growth. In recent years, high-throughput proteomic approaches allowing for proteomic investigation of the pollen of rice, Arabidopsis and gymnosperms have led to characterization of a functional category of proteins expressed in pollen. Proteins involved in wall remodeling and metabolism, protein metabolism, cytoskeletal dynamics and signal transduction are highly represented in pollen, and 25% of proteins appear as multi-isoforms. This paper gives an overview of recent pollen proteomic investigations.