植物学报 ›› 2004, Vol. 21 ›› Issue (02): 201-204.

• 技术与方法 • 上一篇    下一篇

拟南芥室内培养技术

李俊华 张艳春 徐云远 种康 王辉   

  1. 1 (中国科学院植物研究所光合作用与环境分子生理学重点实验室 北京 100093) 2 (华东师范大学生物学系 上海 200062) 3 (西北农林科技大学农学院 杨凌 712100)
  • 收稿日期:2003-03-13 修回日期:2003-06-16 出版日期:2004-04-20 发布日期:2004-04-20
  • 通讯作者: 种康

Modification of Indoor Culture Techniques for Arabidopsis thaliana

LI Jun-Hua ZHANG Yan-Chun XU Yun-Yuan CHONG Kang WANG Hui   

  1. 1(The Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093) 2(Department of Biology, East China Normal University, Shanghai 200062 )
  • Received:2003-03-13 Revised:2003-06-16 Online:2004-04-20 Published:2004-04-20
  • Contact: CHONG Kang

摘要: 本文报道了室内培养拟南芥的一些简便易行的改进技术。采用我们改进的营养土、蛭石、素沙混合培养介质和直播方式培养拟南芥, 并根据其生物学特性在温度、空气湿度、土壤水分和光照等方面给予适当管理,能培养出生长更健壮、更好地满足实验要求的拟南芥植株。此外还介绍了播种、浇水、生育期调节、种子保存、病虫害防治和防混杂等环节的一些技巧措施。与其他培养方法相比,此法不仅简便、效果好,而且适合较简易的培养条件。

Abstract: Arabidopsis thaliana is a model plant in research of genetics, molecular biology and developmental biology. Several culture problems, however, exist in many laboratories of our country. We developed some indoor culture techniques of Arabidopsis for the laboratories. The modified protocol provided an appropriate indoor conditions for Arabidopsis growth and development. It emphasized culture medium, air humidity, temperature and photoperiod.