植物学报 ›› 2001, Vol. 18 ›› Issue (06): 707-713.

• 研究论文 • 上一篇    下一篇

水稻bicoid反义基因转基因植株胚胎发育的形态变化

郑娥香 杨志行 朱治平 傅中滇   

  1. 1(华东师范大学生物系 上海 200062)2 (中国科学院上海植物生理生态研究所 上海 200032)
  • 收稿日期:2000-11-27 修回日期:2001-03-13 出版日期:2001-11-20 发布日期:2001-11-20
  • 通讯作者: 朱治平

The Morphological Changes of Embryo Developmentin Transgenic Rice with Antisense Rice Bicoid-Related Gene

ZHENG E-Xiang YANG Zhi-Xing ZHU Zhi-PingFU Zhong-Dian   

  1. 1(Department of Biology, East China Normal University,Shanghai 200062)2(Shanghai Institute of Plant Physiology and Ecology,The Chinese Academy of Sciences, Shanghai 200032)
  • Received:2000-11-27 Revised:2001-03-13 Online:2001-11-20 Published:2001-11-20
  • Contact: ZHU Zhi-Ping

摘要: Bicoid 是调控果蝇胚胎极性及以后分节发生过程的重要基因,这一基因编码的蛋白是一种转录调控因子,对一组早期胚胎发育过程中的多齿基因的表达具有调控作用,并被称为形态发生剂。在以前的工作中,从水稻cDNA文库中分离了一个同果蝇bicoid 有相当同源性的克隆—Rb24 基因(EMBL登录号:AJ2771380) 。为进一步了解它在水稻中功能,将Rb 的反义基因片段通过农杆菌的介导转入水稻中。通过Gus活性检测和PCR鉴定所获的抗性植株为转基因植株,转基因植株一个很明显的变化就是大约有50%的种子是败育的。通过石蜡切片观察了转基因水稻幼胚的发育情况,结果表明细胞和组织的分化在胚的发育过程中被阻断了。因此Rb基因同控制水稻胚的发育有关。

Abstract: Bicoid is one of the important Drosophila genes involved in the control of embryo polarity and larvae segmentation. The protein encoded by bicoid is a homedomain_containing transcription factor, which organizes a group of polycomb genes expressed in early development of embryo and therefore named morphogen.In our previous work, one of rice cDNA clone, Rb24, which is significantly homologous to Drosophila bicoid gene, has been isolated (EMBL accession number AJ2771380). To work further on its function in rice, the Rb antisense gene fragment was transferred into rice by transduction of Agrobacterium tumefacies.The transgenic plants were characterized by Gus activity and PCR amplification. An obv ious change of transgenic plants was firstly found in seed development. About 50% of the seeds in transgenic plants were aborted. The rice embryo development was investigated by paraffin section,and the results indicated that cell and tissue differentiation was interfered during embryo development. ccordingly, Rb gene should be involved in regulation of rice embryo development.