植物学报 ›› 2001, Vol. 18 ›› Issue (01): 118-120.

• 技术与方法 • 上一篇    下一篇

高度木质化材料的冰冻切片技术

林月惠 李寒冰 贺新强   

  1. (中国科学院植物研究所 北京 100093)
  • 收稿日期:1999-11-24 修回日期:2000-12-07 出版日期:2001-01-20 发布日期:2001-01-20
  • 通讯作者: 林月惠

Application of Cryo_sectioning Technique in Highly Lignified Tissues

LIN Yue-Hui LI Han-Bing HE Xin-Qiang   

  1. (Institute of Botanty, The Chinese Academy of Sciences, Beijing 100093)
  • Received:1999-11-24 Revised:2000-12-07 Online:2001-01-20 Published:2001-01-20
  • Contact: LIN Yue-Hui

摘要: 高度木质化植物材料的切片难度很大,应用常规切片方法不容易取得高质量的图版。本文介绍的冰冻切片方法,不需要进行长时间的软化处理、以及繁琐的脱水、包埋等过程,切片还进行不同的染色或组织化学测定,是一种快速、简捷和有效的制片方法。 其关键步骤是选好甘油浓度, 冰冻温度和展片步骤。

Abstract: It is normally difficult to cut nice sections and prepare good micrographes from the highly_lignified tissues by means of conventional sectioning methods. In this paper, the cryo-sectioning was introduced to overcome the difficulty and it was further proved to be a fast, simple and effective way of section preparation without passing a long process of softening, dehydration and embedding. The crucial steps are to select a proper concentration of glycerin, a suitable temperature of freezing and an appropriate way of section_flatting.