植物学报 ›› 2001, Vol. 18 ›› Issue (01): 118-120.
• 技术与方法 • 上一篇 下一篇
林月惠 李寒冰 贺新强
收稿日期:
修回日期:
出版日期:
发布日期:
通讯作者:
LIN Yue-Hui LI Han-Bing HE Xin-Qiang
Received:
Revised:
Online:
Published:
Contact:
摘要: 高度木质化植物材料的切片难度很大,应用常规切片方法不容易取得高质量的图版。本文介绍的冰冻切片方法,不需要进行长时间的软化处理、以及繁琐的脱水、包埋等过程,切片还进行不同的染色或组织化学测定,是一种快速、简捷和有效的制片方法。 其关键步骤是选好甘油浓度, 冰冻温度和展片步骤。
Abstract: It is normally difficult to cut nice sections and prepare good micrographes from the highly_lignified tissues by means of conventional sectioning methods. In this paper, the cryo-sectioning was introduced to overcome the difficulty and it was further proved to be a fast, simple and effective way of section preparation without passing a long process of softening, dehydration and embedding. The crucial steps are to select a proper concentration of glycerin, a suitable temperature of freezing and an appropriate way of section_flatting.
林月惠 李寒冰 贺新强. 高度木质化材料的冰冻切片技术. 植物学报, 2001, 18(01): 118-120.
LIN Yue-Hui LI Han-Bing HE Xin-Qiang. Application of Cryo_sectioning Technique in Highly Lignified Tissues. Chinese Bulletin of Botany, 2001, 18(01): 118-120.
导出引用管理器 EndNote|Ris|BibTeX
链接本文: https://www.chinbullbotany.com/CN/
https://www.chinbullbotany.com/CN/Y2001/V18/I01/118