植物学报 ›› 2000, Vol. 17 ›› Issue (05): 429-434.

• 研究简报 • 上一篇    下一篇

条斑紫菜中一种琼胶多糖的分离纯化及鉴定

张伟云 陈颢 汪水娟 谭仁祥 费修绠   

  1. (南京大学生物科学与技术系 南京 210093) (中国科学院海洋研究所 青岛 266071)
  • 收稿日期:1999-07-12 修回日期:1999-09-22 出版日期:2000-09-20 发布日期:2000-09-20
  • 通讯作者: 张伟云

The Isolation, Purification and Identification of a Kind ofAgar Polysaccharide from Porphyra yezoensis

ZHANG Wei-Yun CHEN Hao WANG Shui-Juan TAN Ren-Xiang FEI Xiu-Geng   

  1. (Department of Biological Science and Technology, Nanjing University, Nanjing 210093)
  • Received:1999-07-12 Revised:1999-09-22 Online:2000-09-20 Published:2000-09-20
  • Contact: ZHANG Wei-Yun

摘要: 条斑紫菜(Porphyra yezoensis)的热水提取物,经DE-23和Sephadex G-200柱层析纯化,得到一种含微量硫酸基的琼胶精品(PY1)。此多糖经Sepharose 6B柱层析鉴定为单一组分,分子量为220 kD。紫外光谱显示它不含蛋白质、多肽及核酸。红外光谱揭示它含3,6_内醚_半乳糖的特征吸收以及微量的硫酸基。该多糖的水解产物经气相色谱分析,主要由半乳糖及其衍生物组成,有少量岩藻糖存在。通过高磺酸氧化和Smith除解以及13C-NMR谱的分析,该多糖主要由琼胶二糖结构单元和它的生物前体组成,以琼胶二糖为主。

Abstract: The crude polysaccharide was extracted from Porphyra yezoensis with hot water and was then purified by DE-23 and Sephadex G-200 column chromatography. A purified polysaccharides (PY1) was obtained, which contains little sulfate. Its homogeneity was identified with Sepharose 6B column chromatography. The average molecular weight was estimated to be 220 kD. The ultraviolet spectrum showed there is no proteins and nucleic acids in PY1. And the infrared spectrum indicated that there are special absorption of 3,6-anhydro_galactose and very little sulfated absorbance peaks. Galactose and its derivatives were the main constituents by gas chromatography. Through periodate oxidation, Smith degradation and 13C-NMR analysis, PY1 is mainly composed of agarobiose, and a little of its precusor exists as well.