Abstract: The experiments were conducted with Lotus pollen, this paper introduces the steps of the quantitative analysis of computer on the pollen's shapes. After pollen grains were treated with method of acetic acid, the pollen grains were observed and taken picture by using electron microscope, the picture of pollen was treated with computer, and C Language was applied to draw up the programs of computer for calculating the areas of pollen's shapes. The results showed that the quantitative analysis of computer is helpful for quick measuring index of pollen's shapes, this is effective method for numerical taxonomy of pollen.