植物学报 ›› 1999, Vol. 16 ›› Issue (01): 67-71.
• 研究报告 • 上一篇 下一篇
李彦舫 程肖蕊 张亚兰 庞劲松
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LI Yan-Fang, CHENG Xiao-Rui, ZHANG Ya-Lan and PANG Jin-Song
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摘要： 以野大麦(Hordeum brevisubulatum)的幼穗诱导愈伤组织，建立悬浮细胞系，利用胚性悬浮细胞系在不同的酶解条件和不同的酶解液中分离原生质体并进行培养，获得了细胞分裂，形成细胞团。
Abstract: Embryogenic suspension cell line was established from calli which were induced from young inflorescences of wild barley (Hordeum brevisubulatum. After isolated from the suspension cell line under different conditions and enzyme solutions, protoplasts were cultured, cell division and cell cluster were observed.
李彦舫 程肖蕊 张亚兰 庞劲松. 野大麦幼穗原生质体的分离和培养. 植物学报, 1999, 16(01): 67-71.
LI Yan-Fang;CHENG Xiao-Rui;ZHANG Ya-Lan and PANG Jin-Song. Protoplast Isolation and Culture of Young lnfeorescences of Wild Barley. Chinese Bulletin of Botany, 1999, 16(01): 67-71.
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