植物学报 ›› 2012, Vol. 47 ›› Issue (3): 217-225.DOI: 10.3724/SP.J.1259.2012.00217

• 研究报告 • 上一篇    下一篇

H2O2介导的H2S产生参与干旱诱导的拟南芥气孔关闭

王兰香, 侯智慧, 侯丽霞, 赵方贵, 刘新*   

  1. 青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 青岛 266109
  • 收稿日期:2011-09-01 修回日期:2011-12-20 出版日期:2012-05-01 发布日期:2012-07-06
  • 通讯作者: 刘新
  • 基金资助:

    国家自然基金

H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana

Lanxiang Wang, Zhihui Hou, Lixia Hou, Fanggui Zhao, Xin Liu*   

  1. Key Laboratory of Plant Biotechnology in Universities of Shandong, College of Life Science, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2011-09-01 Revised:2011-12-20 Online:2012-05-01 Published:2012-07-06
  • Contact: Xin Liu
  • Supported by:

    the National Science Foundation

摘要: 以野生型拟南芥(Arabidopsis thaliana)及其突变体(atrbohDatrbohFatrbohD/Fatl-cdesatd-cdes)和过表达株系(OEL-CDesOED-CDes)为材料, 利用药理学实验, 结合分光光度法和激光共聚焦显微技术, 探讨硫化氢(hydrogen sulfide, H2S)在干旱诱导的拟南芥气孔关闭中的作用及其与过氧化氢(hydrogen peroxide, H2O2)的关系。结果表明, H2S清除剂次牛磺酸(hypotaurine, HT)及合成抑制剂氨氧基乙酸(aminooxy acetic acid, AOA)、羟胺(hydroxylamine, NH2OH)和丙酮酸钾(potasium pyruvate, C3H3KO3)+氨水(ammonia, NH3)均可不同程度抑制干旱诱导的气孔关闭; 干旱对OEL-CDesOED-CDes植株气孔关闭的诱导作用明显, 而atl-cdesatd-cdes叶片气孔对干旱胁迫反应的敏感性下降; 干旱胁迫能明显增加拟南芥保卫细胞中H2O2水平及叶片中H2S含量, 提高D-/L-半胱氨酸脱巯基酶活性及基因表达量, 而对突变体atrbohDatrbohFatrbohD/F没有显著影响。清除H2O2可减弱干旱胁迫对H2S含量和D-/L-半胱氨酸脱巯基酶活性的诱导效应。研究 结果表明H2S位于H2O2下游参与干旱诱导拟南芥气孔关闭的信号转导过程。

Abstract: Using the Arabidopsis thaliana wild-type; mutants atrbohD, atrbohF, atrbohD/F, atl-cdes, and atd-cdes; and D-/L-cysteine desulfhydrase overexpression plants, we investigated the roles of hydrogen sulfide (H2S) in drought-induced stomatal closure in A. thaliana. We used a pharmacological experiment combined with laser scanning confocal microscopy and spectrophotography. The H2S scavenger hypotaurine and H2S synthesis inhibitors aminooxy acetic acid, hydroxylamine, potasium pyruvate and ammonia all inhibited drought-induced stomatal closure. Drought-induced stomatal closure was greater in overexpression than wild-type plants, with the effect not obvious in atl-cdes and atd-cdes. Moreover, drought stress enhanced the level of hydrogen peroxide (H2O2) in guard cells and increased the content of H2S and the activity and expression of D-/L-cysteine desulfhydrase in leaves but had no significant effect in atrbohD, atrbohF, and atrbohD/F. The H2O2 scavenger decreased drought-induced H2S production and the activity of D-/L-cysteine desulfhydrase in leaves. H2S may function downstream of H2O2 in the signal transduction pathway of drought-induced stomatal closure in A. thaliana.

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