植物学报 ›› 2011, Vol. 46 ›› Issue (4): 396-406.DOI: 10.3724/SP.J.1259.2011.00396

• 研究报告 • 上一篇    下一篇

H2S可能作为H2O2的下游信号介导茉莉酸诱导的蚕豆气孔关闭

侯智慧, 刘菁, 侯丽霞, 李希东, 刘新*   

  1. 青岛农业大学生命科学学院, 青岛 266109
  • 收稿日期:2010-10-26 修回日期:2011-01-25 出版日期:2011-07-01 发布日期:2011-07-01
  • 通讯作者: 刘新
  • 基金资助:

    国家自然基金;山东省自然基金;植物生理学与生物化学国家重点实验室开放课题

H2S May Function Downstream of H2O2 in Jasmonic Acid-induced Stomatal Closure in Vicia faba

Zhihui Hou, Jing Liu, Lixia Hou, Xidong Li, Xin Liu*   

  1. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2010-10-26 Revised:2011-01-25 Online:2011-07-01 Published:2011-07-01
  • Contact: Xin Liu
  • Supported by:

    National Natural Science Foundation of China

摘要: 以蚕豆(Vicia faba)为材料, 利用激光共聚焦显微技术和分光光度技术, 结合药理学实验, 探讨硫化氢(hydrogen sulphide, H2S)和过氧化氢(hydrogen peroxide, H2O2)在茉莉酸(jasmonic acid, JA)调控气孔运动信号转导中的作用。结果表明, H2S合成抑制剂氨氧基乙酸(aminooxy acetic acid, AOA)、羟胺(hydroxylamine, NH2OH)、丙酮酸钾(potasium pyruvate, C3H3KO3) 和氨水(ammonia, NH3), H2O2 清除剂抗坏血酸(ascorbic acid, AsA), 合成抑制剂水杨羟肟酸 (salicylhydroxamic acid, SHAM)、二苯基碘(diphenylene iodonium, DPI)均可逆转JA诱导的气孔关闭效应。JA能够明显提高蚕豆叶片及保卫细胞中的H2O2水平、H2S含量和L-/D-半胱氨酸脱巯基酶活性; H2S合成抑制剂可抑制JA引起的叶片H2S含量的增加; 而H2O2清除剂则可减弱JA对H2S含量变化和L-/D-半胱氨酸脱巯基酶活性的诱导效应。以上结果表明H2S和H2O2均参与了JA诱导的蚕豆气孔关闭, 且H2S(主要由L-/D-半胱氨酸脱巯基酶合成)可能作为H2O2的下游组分参与调控这 一信号转导过程。

Abstract: Pharmacological treatments combined with laser scanning confocal microscopy (LSCM) and spectrophotography were used to study the role of H2S and H2O2 in the signaling transduction during stomatal movement responding to jasmonic acid (JA) in Vicia faba. Inhibitors of H2S synthesis (aminooxy acetic acid, hydroxylamine, and potasium pyruvate + ammonia), the scavenger of H2O2 (ascorbic acid), and the inhibitors of H2O2 synthesis (salicylhydroxamic acid, diphenylene iodonium) all reduced JA-induced stomatal closure. Moreover, JA enhanced H2O2 and H2S levels and L-/D-cysteine desulfhydrase activity in leaves and guard cells. The inhibitors of L-/D-cysteine desulfhydrase diminished JA-induced H2S production in leaves. In addition, H2O2 scavenger decreased H2S level and L-/D-cysteine desulfhydrase activity induced by JA. Therefore, H2S and H2O2 are involved in the signal transduction pathway of JA-induced stomatal closure. L-/D-cysteine desulfhydrase-derived H2S may represent a novel downstream component of the H2O2 signaling cascade during JA-induced stomatal movement in V. faba.

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