梭梭响应高温-干旱交叉胁迫的microRNA鉴定

doi:10.11983/CBB25164

摘要/Abstract

摘要： 高温和干旱交叉胁迫是影响荒漠植物梭梭(Haloxylon ammodendron)幼苗存活的关键环境因子。为探究梭梭microRNA(Ha-miRNA)在高温和干旱交叉胁迫响应中的分子功能，本研究通过小RNA测序技术，鉴定出梭梭幼苗在高温-干旱交叉胁迫下差异表达的5个Ha-miRNA，包括上调的Ha-miR319b、Ha-miR166a、Ha-miR171a和下调的Ha-miR157a、Ha-miR7534。预测分析发现，Ha-miR7534无靶基因，其余4个Ha-miRNA 靶向30个非生物胁迫响应基因，编码TCP转录因子、丙酮酸脱氢酶等关键功能蛋白。qRT-PCR验证显示，4个Ha-miRNA与靶基因存在显著负调控关系。功能富集分析表明，4个Ha-miRNA通过调控细胞有氧代谢、糖代谢等次生代谢途径和胁迫记忆相关通路，协同增强梭梭的交叉胁迫适应性。本研究为阐明荒漠植物逆境适应的表观遗传机制提供了新见解，并为抗逆育种提供了潜在分子靶点。

关键词: 梭梭, 高温, 干旱, 交叉胁迫, microRNA

Abstract: High-temperature and drought co-stress are critical environmental factors affecting the survival of Haloxylon ammodendron seedlings. To investigate the molecular functions of H. ammodendron microRNAs (Ha-miRNAs) in response to combined high-temperature and drought stress, this study employed small RNA sequencing to identify five Ha-miRNAs differentially expressed under co-stress conditions. Among them, Ha-miR319b, Ha-miR166a, and Ha-miR171a were upregulated, while Ha-miR157a and Ha-miR7534 were downregulated. Predictive analysis revealed that Ha-miR7534 has no target genes, while the other four Ha-miRNAs target 30 non-biological stress response genes encoding key functional proteins such as TCP transcription factors and pyruvate dehydrogenase. qRT-PCR validation confirmed a significant negative regulatory relationship between these Ha-miRNAs and their target genes. Functional enrichment analysis revealed that the four Ha-miRNAs synergistically enhance the cross-tolerance adaptation of H. ammodendron by regulating secondary metabolic pathways such as cellular aerobic metabolism and carbohydrate metabolism, as well as stress memory-related pathways. This study provides novel insights into the epigenetic mechanisms underlying desert plant stress adaptation and offers potential molecular targets for stress-resistant crop breeding.

Key words: Haloxylon ammodendron, heat, drought, cross-stress, microRNA

王娅蒙王旭马紫嫣王波. 梭梭响应高温-干旱交叉胁迫的microRNA鉴定. 植物学报, DOI: 10.11983/CBB25164.

xu wang bo wang. Identification and Functional Analysis of microRNAs in Response to Combined Heat-Drought Stress in Haloxylon ammodendron. Chinese Bulletin of Botany, DOI: 10.11983/CBB25164.

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