外源热精胺及其合成抑制剂对乌菜低温胁迫耐性的影响

doi:10.11983/CBB25051

植物学报

外源热精胺及其合成抑制剂对乌菜低温胁迫耐性的影响

冯锦涛¹^†, 解松美¹^†, 王秉娇¹, 汪承刚^{1, 2}, 袁凌云^{1, 2}, 侯金锋^{1, 2}, 陈国户^{1, 2}, 唐小燕^{1, 2}, 王文杰^{1, 2}, 吴建强^{1, 2}^*

¹安徽农业大学园艺学院, 合肥 230036; ²安徽省皖江蔬菜产业技术研究院, 马鞍山 238200

收稿日期:2025-04-01 修回日期:2025-06-30 出版日期:2025-07-21 发布日期:2025-07-21
通讯作者: 吴建强
基金资助:
安徽省高等学校科学研究重点项目(No.2022AH050932)、安徽省重点研究和开发计划基础领域项目(No.2023z04020005)和安徽农业大学人才科研资助项目(No.rc322210)

Effects of Exogenous Thermospermine and Synthesis Inhibitor on Low-temperature Stress Tolerance of Wucai

Feng Jintao¹^†, Xie Songmei¹^†, Wang Bingjiao¹, Wang Chenggang^{1, 2}, Yuan Lingyun^{1, 2}, Hou Jinfeng^{1, 2}, Chen Guohu^{1, 2}, Tang Xiaoyan^{1, 2}, Wang Wenjie^{1, 2}, Wu Jianqiang^{1, 2*}

¹College of Horticulture, Anhui Agricultural University, Hefei 230036; ²Anhui Wanjiang Vegetable Industrial Technology Institute, Ma’anshan 238200

Received:2025-04-01 Revised:2025-06-30 Online:2025-07-21 Published:2025-07-21
Contact: Jian-Qiang WU

摘要/Abstract

摘要： 本研究首先以低温敏感型的高代自交系乌菜材料舒W-1为试材, 进行不同浓度的外源热精胺处理, 发现与单独低温处理相比, 较低浓度的热精胺(0.05和0.1 mmol∙L^–1)处理使电解质渗透率、过氧化氢、超氧阴离子和丙二醛含量不同程度地下降, 干鲜重增加, 而随着热精胺浓度的升高, 其作用减弱; 对测定指标进行隶属函数评价, 所得的平均隶属函数值排名顺序随着浓度的升高而逐渐下降, 0.05 mmol∙L^–1热精胺平均隶属度得分最高, 说明0.05 mmol∙L^–1热精胺能有效缓解低温对乌菜幼苗的伤害。为了进一步分析热精胺是否在乌菜抵御低温胁迫中发挥作用, 通过低温条件下施加热精胺合成抑制剂, 并结合筛选出的适宜浓度热精胺, 对2个低温耐性不同的乌菜高代自交系乌18 (耐低温型)和舒W-1 (低温敏感型)进行处理。结果表明, 与对照相比, 低温胁迫下2个材料的电解质渗透率和丙二醛含量均显著升高, 其中以舒W-1变化幅度更大, 受到的伤害更为严重, 验证了舒W-1为低温敏感型; 低温下添加热精胺合成抑制剂使2个材料均积累更多的过氧化氢和超氧阴离子, 电解质渗透率和丙二醛含量进一步增加, 但除电解质渗透率外, 其他3个指标在乌18中的变化幅度更大, 且超氧化物歧化酶(superoxide dismutase, SOD)和过氧化物酶(peroxidase, POD)活性显著降低; 同时施加热精胺可缓解由抑制剂处理加重的低温胁迫伤害, 说明热精胺与乌菜的低温胁迫耐性密切相关, 其可能通过维持较高的SOD和POD活性来提高低温耐性。

关键词: 乌菜, 低温胁迫, 热精胺

Abstract: INTRODUCTION: Thermospermine, as a structural isomer of spermine, is an important substrate involved in plant growth and development, as well as responses to biotic and abiotic stress.
RATIONALE: At present, there are few reports on the relationships between thermospermine and low-temperature stress, for which a primary analysis of their relationships in wucai was conducted. Firstly, the optimal concentration of exogenous thermospermine alleviating the damage of wucai caused by low temperature was analyzed. Then, thermospermine synthesis inhibitor combined with the selected appropriate concentration of thermospermine was used under low temperature condition in order to further analyze whether thermospermine plays a role in the resistance to low-temperature stress.
RESULTS: At lower concentrations of thermospermine (0.05 and 0.1 mmol·L^–1), electrolyte leakage, hydrogen peroxide, superoxide anion and malondialdehyde contents decreased with different degrees, and the dry and fresh weight increased, while the effects weakened with the increase of thermospermine concentration. The ranking order of the average membership function values obtained decreased gradually with the increase of the concentration, and the score of 0.05 mmol·L^–1 thermospermine was the highest. The addition of thermospermine synthesis inhibitor under low temperature made the two materials with different low-temperature tolerance accumulate more hydrogen peroxide and superoxide anion, electrolyte leakage and malondialdehyde content further increased. In addition, application of thermospermine at the same time can alleviate the damage of low temperature stress aggravated by inhibitor treatment, which was associated with higher activities of SOD and POD.
CONCLUSION: 0.05 mmol·L^–1 thermospermine could effectively alleviate the damage of low temperature on wucai seedlings, and thermospermine synthesis is closely related to the low-temperature stress tolerance of wucai, which may improve low-temperature tolerance through maintaining higher antioxidant enzyme activities, especially SOD and POD.

Phenotype (A), (B), H₂O₂ (C) and •O₂⁻ (D) staining of two wucai materials with different low-temperature stress tolerance (‘W18’, low-temperature tolerant type, and ‘SW-1’, low-temperature sensitive type) under treatment of thermospermine synthesis inhibitor.

Key words: wucai, low-temperature stress, thermospermine

冯锦涛, 解松美, 王秉娇, 汪承刚, 袁凌云, 侯金锋, 陈国户, 唐小燕, 王文杰, 吴建强. 外源热精胺及其合成抑制剂对乌菜低温胁迫耐性的影响. 植物学报, DOI: 10.11983/CBB25051.

Feng Jintao, Xie Songmei, Wang Bingjiao, Wang Chenggang, Yuan Lingyun, Hou Jinfeng, Chen Guohu, Tang Xiaoyan, Wang Wenjie, Wu Jianqiang.

Effects of Exogenous Thermospermine and Synthesis Inhibitor on Low-temperature Stress Tolerance of Wucai. Chinese Bulletin of Botany, DOI: 10.11983/CBB25051.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB25051

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外源热精胺及其合成抑制剂对乌菜低温胁迫耐性的影响

Effects of Exogenous Thermospermine and Synthesis Inhibitor on Low-temperature Stress Tolerance of Wucai

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