低温处理葡萄根系对叶片PSII活性的影响

doi:10.11983/CBB16030

植物学报 ›› 2017, Vol. 52 ›› Issue (2): 159-166.DOI: 10.11983/CBB16030

低温处理葡萄根系对叶片PSII活性的影响

孙鲁龙, 耿庆伟, 邢浩, 杜远鹏, 翟衡^*()

山东农业大学园艺科学与工程学院, 果树生物学国家重点实验室, 泰安 271018

收稿日期:2016-02-22 接受日期:2016-04-26 出版日期:2017-03-01 发布日期:2017-04-05
通讯作者: 翟衡
作者简介:
# 共同第一作者
基金资助:
国家现代葡萄产业技术体系建设专项(No.CARS-30)和长江学者和创新团队发展计划(No.IRT15R42)

Effect of Low Temperature Treatments in Root of Grapevine on PSII Activity in Leaves

Lulong Sun, Qingwei Geng, Hao Xing, Yuanpeng Du, Heng Zhai^*

State Key Laboratory of Crop Biology, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, China

Received:2016-02-22 Accepted:2016-04-26 Online:2017-03-01 Published:2017-04-05
Contact: Zhai Heng
About author:
# Co-first authors

摘要/Abstract

摘要： 以美乐葡萄(Vitis vinifera cv. ‘Merlot’)幼苗为试材, 对叶片进行霜冻胁迫的同时控制土壤降温过程, 造成根系冷胁迫(2°C)和冻胁迫(0°C)。测定霜冻胁迫后和恢复期间叶片的快速叶绿素荧光参数, 并分析低温胁迫不同根系对叶片霜冻害程度的影响。结果表明, 根系在不同低温胁迫下会影响叶片对霜冻的反应, 根系冻胁迫造成叶片严重的霜冻伤害, 光系统II (PSII)反应中心活性难以得到恢复; 根系冷胁迫能避免叶片严重的低温伤害, 低温胁迫后PSII的活性也能很快恢复。

Abstract: Vitis vinifera cv. ‘Merlot’ roots undergo freezing stress (0°C) or chilling stress (2°C) during cold treatment of grapevine leaves. Fast chlorophyll fluorescence was measured after cold treatment and used to analyze the effect of low root temperature on frost damage in leaves. Low root temperature to different extents led to different frost damage on leaves. Freezing stress (0°C) on roots led to more severe damage on leaves with cold treatment; this kind of damage to PSII was unable to recover. Chilling stress (2°C) applied to roots prevented the severe damage to leaves and was beneficial for recovery in PSII.

孙鲁龙, 耿庆伟, 邢浩, 杜远鹏, 翟衡. 低温处理葡萄根系对叶片PSII活性的影响. 植物学报, 2017, 52(2): 159-166.

Lulong Sun, Qingwei Geng, Hao Xing, Yuanpeng Du, Heng Zhai. Effect of Low Temperature Treatments in Root of Grapevine on PSII Activity in Leaves. Chinese Bulletin of Botany, 2017, 52(2): 159-166.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB16030

https://www.chinbullbotany.com/CN/Y2017/V52/I2/159

图/表 6

图1 低温胁迫刚结束时不同根系低温胁迫的葡萄叶片快速叶绿素荧光诱导动力学曲线(O-J-I-P曲线)(A) 叶绿素荧光原始数据; (B) 叶绿素荧光数据标准化后的相对值

Figure 1 Fast chlorophyll fluorescence induction dynamics curves of grape leaves with different low root temperature stress as soon as the frost treatment finished (A) Original data of chlorophyll fluorescence; (B) Standardized data of chlorophyll fluorescence

表1 低温胁迫刚结束时不同根系低温胁迫的葡萄叶片的快速叶绿素荧光参数(平均值±标准误, n=5)

Table 1 Fast chlorophyll fluorescence parameters of grape leaves with different low root temperature stress as soon as the frost treatment finished (means±SE, n=5)

Chlorophyll fluorescence parameters	Control	Freeze	Cool
F_v/F_m	0.75±0.01 a	0.51±0.01 b	0.74±0.01 a
F₀	335.65±7.26 b	551.74±161.76 a	329.55±7.93 b
PI_abs	1.3±0.06 a	0.39±0.12 c	1.13±0.05 b
W_k	0.47±0.01 a	0.51±0.01 a	0.48±0.01 a
V_j	0.5±0.02 b	0.76±0.05 a	0.47±0.02 b
ψ₀	0.5±0.02 a	0.24±0.05 b	0.53±0.02 a
RC/CS_m	557.05±54.61 a	298.99±11.76 c	491.31±39.15 b
ABS/CS_m	1401.52±54.61 a	1176.33±1.9 b	1279.59±39.15 a
TR₀/CS_m	1065.87±48.49 a	604.33±14.68 c	950.04±33.89 b
ET₀/CS_m	544.17±37 a	220±63.65 b	510.73±27.14 a
DI₀/CS_m	335.65±7.26 b	551.74±161.76 a	329.55±7.93 b

图2 不同根系低温处理后葡萄叶片恢复期的光抑制情况(n=5)Fv/Fm: 最大光化学效率; F0: 初始荧光

Figure 2 Photoinhibition of grape leaves during recovery period after different low root temperature treatment (n=5)Fv/Fm: Maximum quantum yield of PSII; F0: Initial fluorescence

图3 不同根系低温处理后葡萄叶片恢复期PSII供体侧伤害情况(n=5)

Figure 3 Damage of donor side of PSII in grape leaves during recovery period after different low root temperature treatments (n=5)

图4 不同根系低温处理后葡萄叶片恢复期PSII受体侧的伤害情况(n=5)Vj: J点可变荧光强度; ψ0: 经过QA进入电子传递链的能量指数

Figure 4 Damage of acceptor side of PSII in grape leaves during recovery period after different low root temperature treatments (n=5)Vj: Relative variable fluorescence at J step; ψ0: The excitation efficiency of electron transport beyond QA

图5 不同根系低温处理后葡萄叶片恢复期PSII反应中心的伤害情况(n=5)PIabs、RC/CSm、ABS/CSm、TR0/CSm、ET0/CSm和DI0/CSm同表1。

Figure 5 Damage of PSII reaction center in grape leaves during recovery period after different low root temperature treatments (n=5)PIabs, RC/CSm, ABS/CSm, TR0/CSm, ET0/CSm, and DI0/CSm see Table 1.

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低温处理葡萄根系对叶片PSII活性的影响

Effect of Low Temperature Treatments in Root of Grapevine on PSII Activity in Leaves

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