蛋白液-液相分离调控植物发育及胁迫应答研究进展

doi:10.11983/CBB23070

摘要/Abstract

摘要： 细胞是生物体结构和功能的基本单位, 有膜或无膜包裹的细胞器在细胞内特定的空间各司其职。然而, 无膜细胞器的形成机制和功能仍有待进一步解析。研究表明, 液-液相分离(LLPS)可以使细胞内的特定分子聚集形成凝聚体, 进而发挥重要作用。随着植物中LLPS研究的不断深入, 相分离形成的凝聚体在植物生长发育和胁迫应答过程中的作用备受关注。该文对蛋白液-液相分离调节植物生长发育以及胁迫应答的研究进展进行综述, 为深入研究LLPS在植物细胞中的工作机制提供参考。

关键词: 液-液相分离, 无膜细胞器, 分子凝聚体

Abstract: Cells are basic units of structure and function for organisms, in which membranous and membraneless organelles exert their respective functions in a given space. However, the mechanism underlying the formation and function of membraneless organelles still requires further investigation. It has been shown that liquid-liquid phase separation (LLPS) of proteins may provide a specific way for biomacromolecules within cells to aggregate and form agglomerates, then proceed to play important roles. With increasing reports on LLPS in plants, great interest has been in exploring the roles of phase separation-forming agglutinates in plant growth and development and immune processes. In this review, we mainly focus on the progress of current studies on the role of protein liquid-liquid phase separation in regulating plant growth and development as well as stress responses, which may provide references for further studies on the mechanism of LLPS in plant cells.

Key words: liquid-liquid phase separation, membraneless organelles, biomolecular condensates

黄鑫华, 刘伟, 田世平, 陈彤. 蛋白液-液相分离调控植物发育及胁迫应答研究进展. 植物学报, 2023, 58(6): 946-955.

Xinhua Huang, Wei Liu, Shiping Tian, Tong Chen. Advances in the Regulation of Protein Liquid-liquid Phase Separation on Development and Stress Responses in Plants. Chinese Bulletin of Botany, 2023, 58(6): 946-955.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB23070

https://www.chinbullbotany.com/CN/Y2023/V58/I6/946

图/表 1

图1 相分离在高等植物成花转变、免疫应答、蛋白质分选和非生物胁迫响应中的作用 IDR: 固有无序区; TMF: 成花转变关键因子; TFAM: TMF家族成员; SA: 水杨酸; NPR1: 水杨酸受体; GBPL: 鸟苷三磷酸酶家族结合蛋白; GDAC: GBPL激活防御的分子凝聚体; STT-ANK: 基质锚蛋白STT的ANK结构域; cpTat: 叶绿体双精氨酸转运; FLOE1: 朊病毒样蛋白, 拟南芥水势传感器; QPS: FLOE1蛋白富含谷氨酰胺、脯氨酸和丝氨酸的IDR; DS: FLOE1蛋白富含天冬氨酸和丝氨酸的IDR

Figure 1 Function of phase separation in flowering transition, immune response, protein sorting and abiotic stress responses of higher plants IDR: Intrinsically disordered regions; TMF: Terminating flower; TFAM: TMF family member; SA: Salicylic acid; NPR1: Salicylic acid receptor; GBPL: Guanylate-binding protein-like GTPase; GDAC: GBPL defense-activated condensate; STT-ANK: ANK domain of stromal ankyrin STT; cpTat: Chloroplast Twin-arginine translocation; FLOE1: Prion-like protein, Arabidopsis thaliana water potential sensor; QPS: The IDR of FLOE1 enriched for glutamine, proline, and serine; DS: The IDR of FLOE1 enriched for aspartic acid and serine

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