十字花科植物ABI4序列演化与转录激活活性分析

doi:10.11983/CBB21036

植物学报 ›› 2021, Vol. 56 ›› Issue (6): 676-686.DOI: 10.11983/CBB21036

十字花科植物ABI4序列演化与转录激活活性分析

张文晶¹, 杨晓萌¹, 高侃¹, 魏欣仪¹, 石雪彤¹, 王瑞瑄¹, 武凤霞², 康菊清¹^,^*()

¹陕西师范大学生命科学学院, 西安 710062
²北京市农林科学院, 北京 100097

收稿日期:2021-02-10 接受日期:2021-08-09 出版日期:2021-11-01 发布日期:2021-11-12
通讯作者: 康菊清
作者简介:* E-mail: kangjq@snnu.edu.cn
基金资助:
国家自然科学基金(31970228);全国大学生创新创业计划(201910718034)

Analysis on the Evolution and Transcription Activation Activity of ABI4 in Brassicaceae

Wenjing Zhang¹, Xiaomeng Yang¹, Kan Gao¹, Xinyi Wei¹, Xuetong Shi¹, Ruixuan Wang¹, Fengxia Wu², Juqing Kang¹^,^*()

¹College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China
²Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2021-02-10 Accepted:2021-08-09 Online:2021-11-01 Published:2021-11-12
Contact: Juqing Kang

1. 附表1 43 种十字花科植物ABI4 同源序列检索结果汇总.pdf(121KB)

摘要/Abstract

摘要：

转录因子ABI4参与植物激素ABA的绝大多数生物学功能, 它不仅是ABA和GA在植物体内含量平衡的核心调控因子, 同时还连接ABA与植物细胞内多个信号通路。利用拟南芥(Arabidopsis thaliana) ABI4序列在十字花科其它19种植物(除拟南芥外)中检索得到27个同源基因, 通过序列多态性分析、系统发生重建、染色体共线性分析和转录激活活性比较, 探讨了该基因在十字花科植物中的演化历史。结果表明, ABI4蛋白质序列和结构在十字花科植物中具有较高的保守性, 暗示了其功能的重要性; 单独的ABI4蛋白并不具备显著的转录激活活性, 说明其生物学功能的具体分子机制可能相对复杂, 需要进一步探讨。

关键词: ABI4, 十字花科, 序列演化, 转录激活活性

Abstract:

ABI4 is an important component of the ABA signal transduction pathway. It not only acts as a key player in ABA and GA antagonism, but also plays important roles in various aspects of ABA crosstalk with other signal chemicals. Genes regulated by ABI4 are involved in diverse processes. In this study, we got 27 homologs from 19 species in the family Brassicaceae using AthABI4 as a query, and explored their evolutionary history based on sequence polymorphism analysis, phylogenetic reconstruction, genomic synteny assay, and transcription activation activity comparison. The result revealed that ABI4 are highly conserved and might be not work as transcriptional activators independently in the Brassicaceae, which implies that the irreplaceable roles of ABI4 in plants and further research was needed to explore the molecular mechanism of its biological function.

Key words: ABI4, Brassicaceae, sequence evolution, transcriptional activation activity

张文晶, 杨晓萌, 高侃, 魏欣仪, 石雪彤, 王瑞瑄, 武凤霞, 康菊清. 十字花科植物ABI4序列演化与转录激活活性分析. 植物学报, 2021, 56(6): 676-686.

Wenjing Zhang, Xiaomeng Yang, Kan Gao, Xinyi Wei, Xuetong Shi, Ruixuan Wang, Fengxia Wu, Juqing Kang. Analysis on the Evolution and Transcription Activation Activity of ABI4 in Brassicaceae. Chinese Bulletin of Botany, 2021, 56(6): 676-686.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB21036

https://www.chinbullbotany.com/CN/Y2021/V56/I6/676

图/表 3

表1 十字花科7个ABI4基因克隆引物序列

Table 1 Primers for 7 ABI4 genes in the Brassicaceae

Primer name	Primer sequence (5'-3')
AthABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCCAACATCAAC
AthABI4R	ATAAGAATGCGGCCGCTTAATAGAATTCCCCCAAGATGGGATCA
AlyABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCCAACAACACC
AlyABI4R	ATAAGAATGCGGCCGCTCAATAGAATTCCCTTCCTCCTTGTTCCTG
CruABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCAAACAACAACAACA
CruABI4R	ATAAGAATGCGGCCGCTCAATAGAATTCTCCCAAGATAGGATCAATGA
BstABI4F	ACGCGTCGACATGGACCCTTTAGCTTCCAAACAACACC
BstABI4R	ATAAGAATGCGGCCGCTCAGTAGAATTCCCCCAAGATAGGATCA
BraABI4#1F	ACGCGTCGACATGGACCCTTTACCTTCCCAACAACAAC
BraABI4#1R	ATAAGAATGCGGCCGCTCAAAAGTCAAGCAAGAAGGGATCAACA
BraABI4#2F	ACGCGTCGACATGGACCCTTTACCTTCCCAACAAGAAC
BraABI4#2R	ATAAGAATGCGGCCGCTCAAATGTCAAGCAAGGGATCAATAAAA
EsaABI4F	ACGCGTCGACATGGACCCTTTACCTTCCCAAAAACACC
EsaABI4R	ATAAGAATGCGGCCGCTCAAAAGTCAAGCAAGAAGGGATCAACA

图1 十字花科植物中ABI4同源序列编码的氨基酸序列一致性和相似性比较上三角示氨基酸序列的一致性, 下三角示氨基酸序列的相似性。Aar: Aethionema arabicum; Aly: 琴叶拟南芥; Ath: 拟南芥; Bst: Boechera stricta; Bca: 埃塞俄比亚芥; Bna: 欧洲油菜; Bol: 野甘蓝; Bra: 芜菁; Csa: 亚麻荠; Cgr: 大花荠菜; Cru: 荠菜; Chi: 碎米荠; Epa: 条叶盐芥; Esa: 小盐芥; Mer: Microthlaspi erraticum; Rra: 野萝卜; Rsa: 萝卜; Sal: 白芥; Sir: 水蒜芥; Tar: 菥蓂

Figure 1 The identity and similarity of the amino acid sequences of ABI4 homologs in the Brassicaceae The upper triangle shows the identity of amino acid sequences and the lower triangle shows the similarity of amino acid sequences. Aar: Aethionema arabicum; Aly: Arabidopsis lyrata; Ath: Ar. thaliana; Bst: Boechera stricta; Bca: Brassica carinata; Bna: Br. napus; Bol: Br. oleracea var. oleracea; Bra: Br. rapa; Csa: Camelina sativa; Cgr: Capsella grandiflora; Cru: Cap. rubella; Chi: Cardamine hirsuta; Epa: Eutrema parvulum; Esa: E. salsugineum; Mer: Microthlaspi erraticum; Rra: Raphanus raphanistrum; Rsa: R. sativus; Sal: Sinapis alba; Sir: Sisymbrium irio; Tar: Thlaspi arvense

图2 十字花科20种植物中ABI4基因的系统发生关系和6个物种中ABI4的转录激活活性 (A) 20种植物中ABI4基因的系统发生关系(分支上的数字表示最大似然法分析过程中的bootstrap值(100次))、蛋白质保守基序预测和染色体定位的共线性分析; (B) 6种植物中ABI4的蛋白结构和转录激活活性的定性和定量检测。物种缩写见图1; Cpa: 番木瓜

Figure 2 Phylogenetic relationships of ABI4's homologs in 20 Brassicaceae species and transcriptional activation activity of ABI4 in 6 species (A) Phylogenetic relationships (the number above the nodes indicate value in 100 represents of maximum likdihood analysis.), protein motif structure prediction and collinearity analysis of genomic mapping of ABI4's homologs in 20 Brassicaceae species; (B) The protein structure, the qualitative and quantitative detection of transcriptional activation activity of ABI4 in 6 species. Species abbreviations shown in Figure 1; Cpa: Carica papaya

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十字花科植物ABI4序列演化与转录激活活性分析

Analysis on the Evolution and Transcription Activation Activity of ABI4 in Brassicaceae

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