植物学报 ›› 2021, Vol. 56 ›› Issue (2): 166-174.DOI: 10.11983/CBB20191
郭宝生, 刘素恩, 赵存鹏, 王兆晓, 王凯辉, 李丹, 刘旭, 杜海英, 耿军义()
收稿日期:
2020-11-26
接受日期:
2021-03-01
出版日期:
2021-03-01
发布日期:
2021-03-17
通讯作者:
耿军义
作者简介:
*E-mail: gengjunyi66@126.com基金资助:
Baosheng Guo, Su’en Liu, Cunpeng Zhao, Zhaoxiao Wang, Kaihui Wang, Dan Li, Xu Liu, Haiying Du, Junyi Geng()
Received:
2020-11-26
Accepted:
2021-03-01
Online:
2021-03-01
Published:
2021-03-17
Contact:
Junyi Geng
摘要: 提高棉花(Gossypium hirsutum)产量兼顾改良纤维品质是棉花育种的重要目标, 而优良种质创新是品种改良的基础。FBP7::iaaM基因能够调控棉花胚珠表皮细胞IAA的含量, 进而促进棉纤维发育的起始。利用含有FBP7::iaaM基因的种质IF1-1, 通过常规杂交育种手段实现了目的基因向骨干亲本的转移, 培育了优良陆地棉种质冀资139, 并用4个不同类型的陆地棉品系对冀资139纤维品质性状进行了杂种优势分析。结果表明, FBP7::iaaM基因及其调控的优良性状可以在骨干育种亲本中传递, 具有较高的育种价值; 转FBP7::iaaM基因的冀资139具有综合性状优良、优质和高衣分等特点; 纤维长度、比强度及马克隆值的遗传主要由基因加性效应控制, 这为其作为骨干亲本的应用提供了理论依据。
郭宝生, 刘素恩, 赵存鹏, 王兆晓, 王凯辉, 李丹, 刘旭, 杜海英, 耿军义. 转FBP7::iaaM基因陆地棉种质冀资139纤维品质性状杂种优势分析. 植物学报, 2021, 56(2): 166-174.
Baosheng Guo, Su’en Liu, Cunpeng Zhao, Zhaoxiao Wang, Kaihui Wang, Dan Li, Xu Liu, Haiying Du, Junyi Geng. Heterosis Analysis of the Fiber Quality in Gossypium hirsutum Germplasm Jizi139 with FBP7::iaaM. Chinese Bulletin of Botany, 2021, 56(2): 166-174.
No. | Lines | Source and characteristics | Fiber length (mm) | Fiber strength (cN/tex) | Micronaire | Uniformity (%) | Elongation (%) |
---|---|---|---|---|---|---|---|
1 | Jimian958 | (Jimian10×(HBT offspring×GK 12))×Jimian22 | 29.1 | 32.0 | 4.6 | 84.2 | 6.7 |
2 | IF1-1 | iaaMtransgenic germplasm of Jimian14 as receptor | 30.1 | 30.3 | 4.3 | 84.2 | 6.5 |
P1 | Line946 | Upland cotton variety | 26.3 | 24.7 | 6.0 | 82.3 | 7.0 |
P2 | 05-198 | Upland cotton variety | 27.8 | 26.6 | 6.3 | 83.3 | 6.5 |
P3 | Line2658 | Jimian20×Ji1316 | 29.6 | 27.2 | 5.5 | 84.6 | 6.6 |
P4 | Line646 | Line571×Line7886, early mature | 30.6 | 30.8 | 5.5 | 86.2 | 6.4 |
P5 | Jizi139 | Jimian958×IF1-1 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
表1 试验材料及其纤维品质特征特性
Table 1 Test materials and their fiber quality characteristics
No. | Lines | Source and characteristics | Fiber length (mm) | Fiber strength (cN/tex) | Micronaire | Uniformity (%) | Elongation (%) |
---|---|---|---|---|---|---|---|
1 | Jimian958 | (Jimian10×(HBT offspring×GK 12))×Jimian22 | 29.1 | 32.0 | 4.6 | 84.2 | 6.7 |
2 | IF1-1 | iaaMtransgenic germplasm of Jimian14 as receptor | 30.1 | 30.3 | 4.3 | 84.2 | 6.5 |
P1 | Line946 | Upland cotton variety | 26.3 | 24.7 | 6.0 | 82.3 | 7.0 |
P2 | 05-198 | Upland cotton variety | 27.8 | 26.6 | 6.3 | 83.3 | 6.5 |
P3 | Line2658 | Jimian20×Ji1316 | 29.6 | 27.2 | 5.5 | 84.6 | 6.6 |
P4 | Line646 | Line571×Line7886, early mature | 30.6 | 30.8 | 5.5 | 86.2 | 6.4 |
P5 | Jizi139 | Jimian958×IF1-1 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
图2 棉花F2群体iaaM基因的检测结果 1 -12: F2单株; +P: 阳性对照IF1-1; -P: 阴性对照冀棉958
Figure 2 The iaaM gene identification in F2 population of cotton 1 -12: F2 individuals; +P: Positive control IF1-1; -P: Negative control Jimian958
Crosses | Fiber length (mm) | Fiber strength (cN/tex) | Micronaire | Uniformity (%) | Elongation (%) | |||||
---|---|---|---|---|---|---|---|---|---|---|
P1×P5 | 29.27±0.34 | 28.23±0.19 | 5.17±0.09 | 84.27±0.74 | 6.67±0.05 | |||||
P5×P1 | 28.9 | 28.6 | 5.2 | 84.6 | 6.7 | |||||
t-value | 1.885 | 1.831 | 0.577 | 0.772 | 1.039 | |||||
P2×P5 | 30.00±0.38 | 28.50±0.26 | 5.40±0.00 | 86.36±0.31 | 6.77±0.06 | |||||
P5×P2 | 29.4 | 29.1 | 5.4 | 83.3 | 6.7 | |||||
t-value | 1.998 | 2.078 | 0 | 4.076* | 2.020 | |||||
P3×P5 | 31.47±0.83 | 30.27±0.40 | 5.03±0.15 | 83.60±0.56 | 6.80±0.10 | |||||
P5×P3 | 30.9 | 29.6 | 4.8 | 85.1 | 6.7 | |||||
t-value | 1.189 | 1.901 | 1.655 | 1.665 | 1.732 | |||||
P4×P5 | 31.57±0.45 | 29.73±0.40 | 5.00±0.17 | 84.40±0.89 | 6.77±0.06 | |||||
P5×P4 | 31.5 | 30.7 | 4.9 | 84.2 | 6.8 | |||||
t-value | 0.269 | 2.100 | 1.019 | 0.389 | 0.866 |
表2 冀资139为亲本的正反交组合纤维品质性状比较
Table 2 Comparison of fiber quality between cross-positive combinations and cross-negative combinations of Jizi139 as parent
Crosses | Fiber length (mm) | Fiber strength (cN/tex) | Micronaire | Uniformity (%) | Elongation (%) | |||||
---|---|---|---|---|---|---|---|---|---|---|
P1×P5 | 29.27±0.34 | 28.23±0.19 | 5.17±0.09 | 84.27±0.74 | 6.67±0.05 | |||||
P5×P1 | 28.9 | 28.6 | 5.2 | 84.6 | 6.7 | |||||
t-value | 1.885 | 1.831 | 0.577 | 0.772 | 1.039 | |||||
P2×P5 | 30.00±0.38 | 28.50±0.26 | 5.40±0.00 | 86.36±0.31 | 6.77±0.06 | |||||
P5×P2 | 29.4 | 29.1 | 5.4 | 83.3 | 6.7 | |||||
t-value | 1.998 | 2.078 | 0 | 4.076* | 2.020 | |||||
P3×P5 | 31.47±0.83 | 30.27±0.40 | 5.03±0.15 | 83.60±0.56 | 6.80±0.10 | |||||
P5×P3 | 30.9 | 29.6 | 4.8 | 85.1 | 6.7 | |||||
t-value | 1.189 | 1.901 | 1.655 | 1.665 | 1.732 | |||||
P4×P5 | 31.57±0.45 | 29.73±0.40 | 5.00±0.17 | 84.40±0.89 | 6.77±0.06 | |||||
P5×P4 | 31.5 | 30.7 | 4.9 | 84.2 | 6.8 | |||||
t-value | 0.269 | 2.100 | 1.019 | 0.389 | 0.866 |
Crossing | Fiber length (mm) | Fiber strength (cN/tex) | Micronaire | Uniformity (%) | Elongation (%) |
---|---|---|---|---|---|
P1×P5 | 29.27±0.34 | 28.23±0.19 | 5.17±0.09 | 84.27±0.74 | 6.67±0.05 |
P1 | 26.3 | 24.7 | 6.0 | 82.3 | 7.0 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.02 | 0.30 | 0.34 | 0.58 | 0.08 |
Over-parent heterosis (%) | -9.10 | -9.23 | -19.76 | 2.27 | -4.71 |
P2×P5 | 30.0±0.38 | 28.5±0.26 | 5.4±0.00 | 86.36±0.31 | 6.77±0.06 |
P2 | 27.8 | 26.6 | 6.3 | 83.3 | 6.5 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.00 | -0.30 | 0.71 | 1.06 | 1.45 |
Over-parent heterosis (%) | -6.83 | -8.36 | -21.43 | 3.71 | 4.29 |
P3×P5 | 31.47±0.83 | 30.27±0.40 | 5.03±0.15 | 83.60±0.56 | 6.80±0.10 |
P3 | 29.6 | 27.2 | 5.5 | 84.6 | 6.6 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.46 | 0.96 | 0.93 | 0.03 | 1.36 |
Over-parent heterosis (%) | -2.27 | -2.67 | -11.19 | -1.21 | 3.17 |
P4×P5 | 31.57±0.45 | 29.73±0.40 | 5.00±0.17 | 84.40±0.89 | 6.77±0.06 |
P4 | 30.6 | 30.8 | 5.5 | 86.2 | 6.4 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.14 | -0.99 | 0.77 | 0.03 | 1.65 |
Over-parent heterosis (%) | -1.96 | -4.41 | -11.90 | -2.18 | 5.87 |
表3 冀资139纤维品质性状杂种优势分析
Table 3 Heterosis analysis of fiber quality traits in Jizi139
Crossing | Fiber length (mm) | Fiber strength (cN/tex) | Micronaire | Uniformity (%) | Elongation (%) |
---|---|---|---|---|---|
P1×P5 | 29.27±0.34 | 28.23±0.19 | 5.17±0.09 | 84.27±0.74 | 6.67±0.05 |
P1 | 26.3 | 24.7 | 6.0 | 82.3 | 7.0 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.02 | 0.30 | 0.34 | 0.58 | 0.08 |
Over-parent heterosis (%) | -9.10 | -9.23 | -19.76 | 2.27 | -4.71 |
P2×P5 | 30.0±0.38 | 28.5±0.26 | 5.4±0.00 | 86.36±0.31 | 6.77±0.06 |
P2 | 27.8 | 26.6 | 6.3 | 83.3 | 6.5 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.00 | -0.30 | 0.71 | 1.06 | 1.45 |
Over-parent heterosis (%) | -6.83 | -8.36 | -21.43 | 3.71 | 4.29 |
P3×P5 | 31.47±0.83 | 30.27±0.40 | 5.03±0.15 | 83.60±0.56 | 6.80±0.10 |
P3 | 29.6 | 27.2 | 5.5 | 84.6 | 6.6 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.46 | 0.96 | 0.93 | 0.03 | 1.36 |
Over-parent heterosis (%) | -2.27 | -2.67 | -11.19 | -1.21 | 3.17 |
P4×P5 | 31.57±0.45 | 29.73±0.40 | 5.00±0.17 | 84.40±0.89 | 6.77±0.06 |
P4 | 30.6 | 30.8 | 5.5 | 86.2 | 6.4 |
P5 | 32.2 | 31.1 | 4.2 | 82.4 | 6.3 |
MP heterosis (%) | 0.14 | -0.99 | 0.77 | 0.03 | 1.65 |
Over-parent heterosis (%) | -1.96 | -4.41 | -11.90 | -2.18 | 5.87 |
Crossing | Fiber lint (%) | Bell weight (g) | Seed index (g) | Fusarium wilt index | Verticillium wilt index |
---|---|---|---|---|---|
P1×P5 | 41.5 | 5.8 | 10.9 | 4.3 | 29.0 |
P1 | 38.6 | 5.1 | 11.3 | 2.3 | 29.5 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | 1.97 | 8.41 | -4.80 | 24.64 | 1.03 |
Over-parent heterosis (%) | -3.04 | 3.57 | -6.03 | -6.52 | -0.34 |
P2×P5 | 40.2 | 6.2 | 10.9 | 4.7 | 29.5 |
P2 | 37.1 | 6.3 | 11.1 | 2.1 | 30.6 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | 0.63 | 4.20 | -3.96 | 40.30 | 4.61 |
Over-parent heterosis (%) | -6.07 | -1.59 | -6.03 | 2.17 | -1.21 |
P3×P5 | 42.2 | 6.1 | 10.4 | 10.3 | 24.3 |
P3 | 41.2 | 5.9 | 10.1 | 8.0 | 23.8 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | 0.48 | 6.09 | -4.15 | 63.49 | -7.43 |
Over-parent heterosis (%) | -1.40 | 3.39 | -10.34 | 28.75 | -15.33 |
P4×P5 | 40.2 | 6.2 | 10.7 | 4.0 | 26.3 |
P4 | 39.4 | 5.6 | 10.9 | 3.8 | 21.3 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | -2.19 | 10.71 | -4.89 | -4.76 | 5.20 |
Over-parent heterosis (%) | -6.07 | 10.71 | -7.76 | -13.04 | -8.36 |
表4 冀资139主要经济性状杂种优势分析
Table 4 Heterosis analysis of main economic traits in Jizi139
Crossing | Fiber lint (%) | Bell weight (g) | Seed index (g) | Fusarium wilt index | Verticillium wilt index |
---|---|---|---|---|---|
P1×P5 | 41.5 | 5.8 | 10.9 | 4.3 | 29.0 |
P1 | 38.6 | 5.1 | 11.3 | 2.3 | 29.5 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | 1.97 | 8.41 | -4.80 | 24.64 | 1.03 |
Over-parent heterosis (%) | -3.04 | 3.57 | -6.03 | -6.52 | -0.34 |
P2×P5 | 40.2 | 6.2 | 10.9 | 4.7 | 29.5 |
P2 | 37.1 | 6.3 | 11.1 | 2.1 | 30.6 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | 0.63 | 4.20 | -3.96 | 40.30 | 4.61 |
Over-parent heterosis (%) | -6.07 | -1.59 | -6.03 | 2.17 | -1.21 |
P3×P5 | 42.2 | 6.1 | 10.4 | 10.3 | 24.3 |
P3 | 41.2 | 5.9 | 10.1 | 8.0 | 23.8 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | 0.48 | 6.09 | -4.15 | 63.49 | -7.43 |
Over-parent heterosis (%) | -1.40 | 3.39 | -10.34 | 28.75 | -15.33 |
P4×P5 | 40.2 | 6.2 | 10.7 | 4.0 | 26.3 |
P4 | 39.4 | 5.6 | 10.9 | 3.8 | 21.3 |
P5 | 42.8 | 5.6 | 11.6 | 4.6 | 28.7 |
MP heterosis (%) | -2.19 | 10.71 | -4.89 | -4.76 | 5.20 |
Over-parent heterosis (%) | -6.07 | 10.71 | -7.76 | -13.04 | -8.36 |
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