植物学报 ›› 2018, Vol. 53 ›› Issue (3): 305-312.DOI: 10.11983/CBB18027

所属专题: 药用植物专辑

• 研究报告 • 上一篇    下一篇

基于SSR分子标记的酸浆属植物亲缘关系研究

朱宇佳1, 焦凯丽1, 罗秀俊1, 冯尚国1,2,*(), 王慧中1,2,*()   

  1. 1杭州师范大学生命与环境科学学院, 浙江省药用植物种质改良和质量监控重点实验室, 杭州 311121
    2湖南农业大学生物科学技术学院, 长沙 410128
  • 收稿日期:2018-01-28 接受日期:2018-05-09 出版日期:2018-05-01 发布日期:2018-06-05
  • 通讯作者: 冯尚国,王慧中
  • 基金资助:
    国家自然科学基金(No.31470407)、浙江省科技计划(No.2014C32090)和杭州市科技局项目(No.20150932H04, No.20150932H03)

Genetic Relationship of Physalis Plants Revealed by Simple Sequence Repeat Markers

Zhu Yujia1, Jiao Kaili1, Luo Xiujun1, Feng Shangguo1,2,*(), Wang Huizhong1,2,*()   

  1. 1Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China
    2College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha 410128, China;
  • Received:2018-01-28 Accepted:2018-05-09 Online:2018-05-01 Published:2018-06-05
  • Contact: Feng Shangguo,Wang Huizhong

摘要:

近年来, 由于营养价值高、果实可当水果食用且具有潜在的药用价值, 酸浆属(Physalis)植物在全球范围内受到了越来越多的关注。采用SSR分子标记技术对我国范围内主要分布的4种酸浆属植物的22份样品进行了亲缘关系研究, 结果表明, 20对SSR引物共扩增出118个位点, 其中107个(90.7%)为多态性位点; 平均种间遗传相似系数为0.501。UPGMA聚类和PCoA分析结果得出相似结论, 并且将供试酸浆属植物样品分为两大类。其中, 酸浆(P. alkekengi var. francheti)的所有样品与其它酸浆属植物的遗传距离最远, 单独聚为一类, 与前人的研究结果非常吻合。研究表明, SSR标记遗传信息丰富, 可以用于酸浆属植物的亲缘关系分析。研究结果为酸浆属种质资源保护提供了有效信息, 并且为酸浆属植物的分子辅助育种奠定了重要基础。

关键词: 酸浆属, 亲缘关系, SSR分子标记

Abstract:

In recent years, Physalis plants have attracted increasing attention worldwide due to their high nutritional value, edible fruit, and potential medicinal value. In this study, simple sequence repeat (SSR) markers were used to assess genetic relationships with 22 samples of four Physalis species mainly distributed in China. Twenty SSR primer pairs produced 118 loci, 90.7% (107) of which showed polymorphism. The average interspecies similarity coefficient was 0.501, which indicates a degree of genetic relationship among Physalis species. The results of UPGMA dendrography and PCoA plotting were similar, and all Physalis samples were grouped into two clusters. All P. alkekengi var. francheti samples, distant from any other Physalis species, constituted a separate cluster, which confirmed findings of previous studies. This study also indicated that SSR markers are rich in genetic information and could be used to assess the genetic diversity of Physalis species which provides rich useful information for protecting the Physalis germplasm resource and an important foundation for molecular assisted-breeding programs with Physalis.

Key words: Physalis, genetic relationship, SSR markers