基于孢子形态和分子证据探讨鳞盖蕨属(碗蕨科)系统分类

doi:10.11983/CBB17258

植物学报 ›› 2018, Vol. 53 ›› Issue (6): 782-792.DOI: 10.11983/CBB17258

基于孢子形态和分子证据探讨鳞盖蕨属(碗蕨科)系统分类

罗俊杰^1,², 王莹^1,², 商辉^1,³, 周喜乐⁴, 韦宏金^1,³, 黄素楠², 顾钰峰^1,², 金冬梅^1,³, 戴锡玲², 严岳鸿^1,^3,^*()

¹上海辰山植物园, 中国科学院上海辰山植物科学研究中心, 上海 201602
²上海师范大学生命与环境科学学院, 上海 200234
³国家林业局华东野生濒危资源植物保育中心, 上海 201602
⁴湘西自治州森林资源监测中心, 吉首 416000

收稿日期:2017-12-29 出版日期:2018-11-01 发布日期:2018-12-05
通讯作者: 严岳鸿
作者简介:
作者简介:白克智, 1959年开始在中国科学院植物研究所工作, 先后任助理研究员、研究员, 长期从事植物生长发育及其调控的研究。1986年,其主持的“满江红生物学特性研究”荣获中国科学院科技进步二等奖。曾任《植物生理学报》编委、《植物学报》常务编委、中国植物生长调节剂协会主任等职。
基金资助:
国家自然科学基金(No.31370234)、上海市绿化和市容管理局科技攻关项目(No.G162401)和科技部科技基础性工作专项(No. 2013FY112100)

Phylogeny and Systematics of the Genus Microlepia (Dennstaedtiaceae) based on Palynology and Molecular Evidence

Luo Junjie^1,², Wang Ying^1,², Shang Hui^1,³, Zhou Xile⁴, Wei Hongjin^1,³, Huang Sunan², Gu Yufeng^1,², Jin Dongmei^1,³, Dai Xiling², Yan Yuehong^1,^3,^*()

¹Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, China
²College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
³Eastern China Conservation Center for Wild Endangered Plant Resources, State Forestry Administration, Shanghai 201602, China
⁴Xiangxi Autonomous Prefecture Forest Resources Monitoring Center, Jishou 416000, China

Received:2017-12-29 Online:2018-11-01 Published:2018-12-05
Contact: Yan Yuehong

1. 基于孢子形态和分子证据探讨鳞盖蕨属(碗蕨科)系统分类附录.pdf(11861KB)

摘要/Abstract

摘要： 孢粉学是解决植物分类中疑难类群物种微形态分化的重要方法, 随着分子系统学的发展, 结合这两门学科的优势可以更加有效地解决疑难类群的分类学问题。鳞盖蕨属(Microlepia)是一个分类困难的疑难类群, 采用孢粉学与分子系统学一一对应的方法, 以及居群取样方式, 选取280份样本, 联合4个叶绿体片段(rbcL、trnL-F、psbA-trnH和rps4), 采用最大似然法和贝叶斯法构建该属的系统发生关系, 在此基础上对凭证标本中100份材料的孢子进行观察和分析。综合分子系统学和孢粉学的研究结果, 得出结论: (1) 在形态学研究中广泛被接受的15个物种得到了单系支持, 并厘清了分类困难的复合群; (2) 发现边缘鳞盖蕨(M. marginata)可能存在隐性种; (3) 建议恢复过去归并处理为异名的瑶山鳞盖蕨(M. yaoshanica)、罗浮鳞盖蕨(M. lofoushanensis)、四川鳞盖蕨(M. szechuanica)以及滇西鳞盖蕨(M. subspeluncae); (4) 提出鳞盖蕨属可能存在杂交现象; (5) 提出鳞盖蕨属完整的属下分类建议。

关键词: 隐性种, 杂交, 孢粉学, 分子系统学, 蕨类植物, 分类

Abstract: Palynology is an important method to solve the micro-morphological differentiation of species in some complex groups. With the development of molecular phylogeny, combining the advantages of these two subjects may effectively solve the taxonomic issues in plants. Microlepia (Dennstaedtiaceae) is one of the most difficult genera in ferns in terms of taxonomy. In the present study, based on palynology matched with phylogeny and population sampling, we constructed the phylogeny of 280 samples by using both Maximum Likelihood and Bayesian methods with four plastid markers (rbcL, trnL-F, psbA-trnH and rps4). The spore morphology of 100 samples was observed and analyzed. Our results of comprehensive molecular phylogeny and palynology showed that (1) 15 species widely accepted based on plant morphology were strongly supported, and this complex group of Microlepia has been further clarified; (2) There may be crytic species in the populations of M. marginata; (3) M. yaoshanica Ching, M. lofoushanensis Ching, M. szechuanica Ching and M. subspeluncae Ching, treated as synonyms in previous studies, should be restored as independent species; (4) Many hybridization events of Microlepia were found; and (5) A complete infrageneric taxonomy of Microlepia was proposed.

Key words: crytic species, hybrids, palynology, phylogeny and systematics, pteridophytes, taxonomy

罗俊杰, 王莹, 商辉, 周喜乐, 韦宏金, 黄素楠, 顾钰峰, 金冬梅, 戴锡玲, 严岳鸿. 基于孢子形态和分子证据探讨鳞盖蕨属(碗蕨科)系统分类. 植物学报, 2018, 53(6): 782-792.

Luo Junjie, Wang Ying, Shang Hui, Zhou Xile, Wei Hongjin, Huang Sunan, Gu Yufeng, Jin Dongmei, Dai Xiling, Yan Yuehong. Phylogeny and Systematics of the Genus Microlepia (Dennstaedtiaceae) based on Palynology and Molecular Evidence. Chinese Bulletin of Botany, 2018, 53(6): 782-792.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB17258

https://www.chinbullbotany.com/CN/Y2018/V53/I6/782

图/表 2

图 1 基于4个叶绿体片段rbcL、trnL-F、psbA-trnH和rps4联合数据构建的鳞盖蕨属贝叶斯系统发育树分支旁边的数字表示贝叶斯后验概率与ML自展支持率(≥50%); * 表示1或100; - 表示<0.5或50%。

Figure 1 Bayesian inference phylogeny of Microlepia derived from the combined data (rbcL, trnL-F, psbA-trnH and rps4) Values beside each branch represent bootstrap support for parsimony, Bayesian posterior probabilities and maximum likelihood (≥50%); * means 1 or 100; - means <0.5 or 50%.

图 2 孢子大小分布对应鳞盖蕨属的谱系分化

Figure 2 Distribution of spore size corresponded to phylogeny differentiation of Microlepia

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基于孢子形态和分子证据探讨鳞盖蕨属(碗蕨科)系统分类

Phylogeny and Systematics of the Genus Microlepia (Dennstaedtiaceae) based on Palynology and Molecular Evidence

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