植物学报 ›› 2017, Vol. 52 ›› Issue (2): 218-224.DOI: 10.11983/CBB16033

• 技术方法 • 上一篇    下一篇

氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响

陈曼, 涂艺声*(), 叶丽婻, 杨碧芸   

  1. 江西师范大学生命科学学院, 南昌 330022
  • 收稿日期:2016-02-24 接受日期:2016-09-04 出版日期:2017-03-01 发布日期:2017-04-05
  • 通讯作者: 涂艺声
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    国家自然科学基金(No.81360614, No.31370390, No.81660597)、江西省自然科学基金(No.20132BAB204023)和江西省科技支撑项目(No.20121BBG70016)

Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata

Man Chen, YishengTu*, Linan Ye, Biyun Yang   

  1. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
  • Received:2016-02-24 Accepted:2016-09-04 Online:2017-03-01 Published:2017-04-05
  • Contact: YishengTu
  • About author:

    # Co-first authors

摘要:

通过在培养基中添加不同浓度的天冬氨酸、赖氨酸和色氨酸, 探究其对蛇足石杉(Huperzia serrata)叶状体生长、石杉碱甲积累及SOD酶活性的影响。结果表明: 添加天冬氨酸后, 叶状体干重生物量比对照提高18.50%-25.75%, 石杉碱甲含量亦明显高于对照, 其最适天冬氨酸浓度为0.5 mmol·L-1; 添加1 mmol·L-1色氨酸后, 叶状体相对增殖率显著高于对照, 但其石杉碱甲积累量略低于对照; 添加赖氨酸后, 叶状体相对增殖量、干重生物量及石杉碱甲含量均低于对照。添加一定浓度的天冬氨酸使SOD酶活性适当提高, 同时叶状体石杉碱甲积累量增加, 而长期保持高水平的SOD酶活性对叶状体积累石杉碱甲不利。

Abstract:

We investigated the effects of amino acid supplements on thallus growth, superoxide dismutase (SOD) activity and huperzine-A accumulation in Huperzia serrata by adding aspartic acid, lysine, and tryptophan at different concentrations into the medium. With aspartic acid, the dry weight of the thallus increased by 18.50%-25.75% and the huperzine-A accumulation was significantly higher than in the control. The optimal concentration of aspartic acid was 0.5 mmol·L-1. The relative growth of thallus was significantly higher with 1 mmol·L-1 tryptophan than in the control, but the huperzine-A accumulation was slightly lower than for the control. The relative growth rate, dry weight and huperzine-A accumulation was lower for thallus cultured with lysine than the control. The SOD activity of the thallus cultured with aspartic acid was increased properly, and the huperzine-A accumulation of the thallus was increased. However, huperzine-A accumulation of the thallus was suppressed when SOD activity remained high in the long-term.