以黄花乌头发根为外植体的再生培养体系建立

doi:10.11983/CBB14174

植物学报 ›› 2015, Vol. 50 ›› Issue (5): 623-627.DOI: 10.11983/CBB14174

以黄花乌头发根为外植体的再生培养体系建立

刘莉莉, 卢淑波, 徐佳萍, 张庆田, 李昌禹^*()

中国农业科学院特产研究所, 长春 130112

收稿日期:2014-09-22 接受日期:2015-03-30 出版日期:2015-09-01 发布日期:2015-10-09
通讯作者: 李昌禹
作者简介:
? 共同第一作者
基金资助:
吉林省科学技术厅自然科学基金(No.201115131)

Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum

Lili Liu, Shubo Lu, Jiaping Xu, Qingtian Zhang, Changyu Li^*

Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2014-09-22 Accepted:2015-03-30 Online:2015-09-01 Published:2015-10-09
Contact: Li Changyu
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 为获得黄花乌头(Aconitum coreanum)发根再生植株, 将其发根在添加0.5-5.0 mg·L^-1 6-BA及0-0.5 mg·L^-1 NAA的1/2MS固体培养基上进行光诱导培养, 发根经脱分化形成愈伤组织, 然后形成芽及芽丛, 最终获得发根再生植株。结果表明, 愈伤组织及不定芽分化的最适培养基为1/2MS+2.0 mg·L^-16-BA+0.2 mg·L^-1NAA+3%蔗糖; 不定芽生根最适培养基为1/2MS+0.5 mg·L^-16-BA+0.1 mg·L^-1NAA+5 mg·L^-1GA+3%蔗糖。

刘莉莉, 卢淑波, 徐佳萍, 张庆田, 李昌禹. 以黄花乌头发根为外植体的再生培养体系建立. 植物学报, 2015, 50(5): 623-627.

Lili Liu, Shubo Lu, Jiaping Xu, Qingtian Zhang, Changyu Li. Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum. Chinese Bulletin of Botany, 2015, 50(5): 623-627.

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https://www.chinbullbotany.com/CN/Y2015/V50/I5/623

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参考文献 11

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2	侯丽丽, 施和平, 余武, 曾宝强, 周卓辉 (2014). 烟草毛状根多倍体诱导及其植株再生. 生物工程学报 30, 581-594.
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5	李昌禹, 王英平, 臧埔, 卢淑波, 宋婷婷 (2005). 黄花乌头叶片诱导植株再生研究. 特产研究 27(2), 34-36.
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9	?
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No. of medi- um	Phytohormone composition (mg·L^-1)		Color change of hairy roots	Callus induced	Adventitious buds induced
No. of medi- um	NAA	6-BA	Color change of hairy roots	Callus induced	Adventitious buds induced
M1	0	0.5	10% turned green	-	-
M2	0	1.0	30% turned green	-	-
M3	0	2.0	10% could see green spots	-	-
M4	0	4.0	5% could see green spots	-	-
M5	0	5.0	5% could see green spots	-	-
M6	0.1	0.5	50% turned green	-	3%
M7	0.2	0.5	60% turned yellow-green	20%	Few adventitious buds were induced
M8	0.5	0.5	40% turned yellow-green	20%	-
M9	0.1	1.0	90% turned yellow-green	-	10%
M10	0.2	1.0	40% turned yellow-green, 20% turned green	25%	Few adventitious buds were induced
M11	0.5	1.0	50% turned yellow-green	30%	Few adventitious buds were induced
M12	0.1	2.0	30% turned yellow-grown	30%	30%
M13	0.2	2.0	40% turned yellow-grown, 20% turned green	-	-
M14	0.5	2.0	40% turned yellow-green	Few callus were obtained	-
M15	0.1	4.0	30% turned green	-	-
M16	0.2	4.0	30% turned yellow-green	20%	40%
M17	0.5	4.0	40% turned yellow-green	20%	-
M18	0.1	5.0	20% turned green	-	-
M19	0.2	5.0	50% turned yellow-green, 50% turned green	50%	Few adventitious buds were induced
M20	0.5	5.0	50% turned yellow-green	20%	20%

No. of medi- um	Phytohormone composition (mg·L^-1)		Color change of hairy roots	Callus induced	Adventitious buds induced
No. of medi- um	NAA	6-BA	Color change of hairy roots	Callus induced	Adventitious buds induced
M1	0	0.5	10% turned green	-	-
M2	0	1.0	30% turned green	-	-
M3	0	2.0	10% could see green spots	-	-
M4	0	4.0	5% could see green spots	-	-
M5	0	5.0	5% could see green spots	-	-
M6	0.1	0.5	50% turned green	-	3%
M7	0.2	0.5	60% turned yellow-green	20%	Few adventitious buds were induced
M8	0.5	0.5	40% turned yellow-green	20%	-
M9	0.1	1.0	90% turned yellow-green	-	10%
M10	0.2	1.0	40% turned yellow-green, 20% turned green	25%	Few adventitious buds were induced
M11	0.5	1.0	50% turned yellow-green	30%	Few adventitious buds were induced
M12	0.1	2.0	30% turned yellow-grown	30%	30%
M13	0.2	2.0	40% turned yellow-grown, 20% turned green	-	-
M14	0.5	2.0	40% turned yellow-green	Few callus were obtained	-
M15	0.1	4.0	30% turned green	-	-
M16	0.2	4.0	30% turned yellow-green	20%	40%
M17	0.5	4.0	40% turned yellow-green	20%	-
M18	0.1	5.0	20% turned green	-	-
M19	0.2	5.0	50% turned yellow-green, 50% turned green	50%	Few adventitious buds were induced
M20	0.5	5.0	50% turned yellow-green	20%	20%

No. of medium	Phytohormone composition (mg·L^-1)				Description of hairy root growing and differentiation status
No. of medium	NAA		6-BA
M6	0.1		0.5		The material turned albino and died
M7	0.2		0.5		30% of the material turned brown, 10% turned green, 30% turned yellow slim hairy roots, 10% produced cluster buds
M8		0.5		0.5		80% turned yellow slim hairy roots, the base section of hairy roots became enlargement and 10% base section turned green
M9	0.1		1.0		The material turned albino and died
M10	0.2		1.0		The material turned albino and died
M11		0.5		1.0		30% base section of hairy roots turned green, 20% new produced hairy roots grew long and thin, others were short and thick
M12		0.1		2.0		30% of the material turned green, 30% kept yellow-green and enlargement
M13		0.2		2.0		The material turned albino and died
M14		0.5		2.0		50% of the material turned green and was going to be buds, hairy roots grew thick, 40% hairy roots were becoming brown
M15		0.1		4.0		40% of the material turned green and some were going to be buds, hairy roots were enlargement and strong, 50% were becoming brown
M16		0.2		4.0		The material turned albino and died
M17		0.5		4.0		The material turned albino and died
M18		0.1		5.0		The material turned albino and died
M19		0.2		5.0		40% of the material turned green, 20% kept yellow-green and grew strongly, 20% turned brown
M20	0.5		5.0		50% of the material turned green and grew strongly, 10% became cluster plantlets, 20% turned brown

No. of medium	Phytohormone composition (mg·L^-1)				Description of hairy root growing and differentiation status
No. of medium	NAA		6-BA
M6	0.1		0.5		The material turned albino and died
M7	0.2		0.5		30% of the material turned brown, 10% turned green, 30% turned yellow slim hairy roots, 10% produced cluster buds
M8		0.5		0.5		80% turned yellow slim hairy roots, the base section of hairy roots became enlargement and 10% base section turned green
M9	0.1		1.0		The material turned albino and died
M10	0.2		1.0		The material turned albino and died
M11		0.5		1.0		30% base section of hairy roots turned green, 20% new produced hairy roots grew long and thin, others were short and thick
M12		0.1		2.0		30% of the material turned green, 30% kept yellow-green and enlargement
M13		0.2		2.0		The material turned albino and died
M14		0.5		2.0		50% of the material turned green and was going to be buds, hairy roots grew thick, 40% hairy roots were becoming brown
M15		0.1		4.0		40% of the material turned green and some were going to be buds, hairy roots were enlargement and strong, 50% were becoming brown
M16		0.2		4.0		The material turned albino and died
M17		0.5		4.0		The material turned albino and died
M18		0.1		5.0		The material turned albino and died
M19		0.2		5.0		40% of the material turned green, 20% kept yellow-green and grew strongly, 20% turned brown
M20	0.5		5.0		50% of the material turned green and grew strongly, 10% became cluster plantlets, 20% turned brown

Phytohormone composition (mg·L^-1)	No. of root
Phytohormone composition (mg·L^-1)	0.1	0.5	1.0	1.5
IBA	1	2	6	6
NAA	5	3	1	0
IAA	0	1	3	4
0.5 mg·L^-16-BA+NAA	4	3	2	1
0.5 mg·L^-16-BA +0.5 mg·L^-1GA+ NAA	8	3	1	0

以黄花乌头发根为外植体的再生培养体系建立

Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum

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