LysM蛋白介导植物免疫防卫反应及其信号激发的研究进展

doi:10.11983/CBB14119

植物学报 ›› 2015, Vol. 50 ›› Issue (5): 628-636.DOI: 10.11983/CBB14119

LysM蛋白介导植物免疫防卫反应及其信号激发的研究进展

季东超, 宋凯, 邢晶晶, 陈彤^*(), 田世平

中国科学院植物研究所北方资源植物重点实验室, 北京 100093

收稿日期:2014-07-14 接受日期:2014-11-13 出版日期:2015-09-01 发布日期:2015-10-09
通讯作者: 陈彤
作者简介:
? 共同第一作者
基金资助:
国家重点基础研究发展计划(No.2013CB127103)和国家自然科学基金(No.31300249, No.31000092)

Studies of Innate Immunity Mediated by Lysin Motif Protein and Its Signaling Priming

Dongchao Ji, Kai Song, Jingjing Xing, Tong Chen^*, Shiping Tian

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2014-07-14 Accepted:2014-11-13 Online:2015-09-01 Published:2015-10-09
Contact: Chen Tong
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要：

溶解素基序(LysM)是一类普遍存在于大多数有机体中的蛋白质结构域。植物细胞中含有LysM结构域的蛋白能够识别不同种类含有N-乙酰葡糖胺结构的配体分子, 从而启动植物对病原菌的特异防御反应。作为一种重要的模式识别受体, LysM结构域蛋白通过不同形式的寡聚化、受体类胞质激酶BIK1和MAPK级联反应向下游传递信号, 而病原菌能够通过其分泌的效应蛋白特异性识别或修饰模式识别受体, 规避植物细胞中病原体相关分子模式诱导的免疫反应。该文主要综述受体激酶/蛋白在病原菌激发子识别和防卫反应启动中的作用。

Abstract:

Lysin motif (LysM) is a ubiquitous protein domain found in most living organisms. LysM-containing proteins in plant cells can recognize different GlcNAc-containing ligands, thereby triggering specific defense responses to pathogens. As a group of important pattern recognition receptors, LysM-containing proteins trigger downstream signaling by oligomerization, thus interacting with the Botrytis-induced kinase 1 (BIK1) and mitogen-activated protein kinase (MAPK) cascade. Pathogenic microorganisms also use certain mechanisms to escape from pathogen-associated molecular pattern-triggered immunity by utilizing specific recognition or modification of pattern recognition receptors by the secreted effector proteins. This review focuses on the functions of these receptor kinases/proteins in response to elicitor stimuli and priming of defense responses.

季东超, 宋凯, 邢晶晶, 陈彤, 田世平. LysM蛋白介导植物免疫防卫反应及其信号激发的研究进展. 植物学报, 2015, 50(5): 628-636.

Dongchao Ji, Kai Song, Jingjing Xing, Tong Chen, Shiping Tian. Studies of Innate Immunity Mediated by Lysin Motif Protein and Its Signaling Priming. Chinese Bulletin of Botany, 2015, 50(5): 628-636.

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图/表 2

表1 拟南芥基因组中的LysM受体激酶/蛋白(Mendes et al., 2011)

Table 1 LysM-containing receptors / proteins in Arabidopsis (Mendes et al., 2011)

基因名称 (别称)	基因号	LysM结构域布局	配体	受体类型	是否为功能性激酶(激酶类型)	突变体对几丁质处理后的响应	备注
AtCERK1 (AtLYK1, LysM RLK1)	At3g21630	I + II + IV	几丁质	LysM-RLK-I LYK	是 (RD kinase)	不敏感	参与肽聚糖感知; LjNFR1横向同源
AtLYK2	At3g01840	* + * + V	未知	LysM-RLK-II LYR	否 (Pseudo kinase)	正常	LjNFR5横向同源II
AtLYK3	At1g51940	* + VII + *	未知	LysM-RLK-I LYK	是 (RD kinase)	正常
AtLYK4	At2g23770	I + II + III	几丁质	LysM-RLK-II LYR	否 (Pseudo kinase)	中等敏感	LjNFR5横向同源I
AtLYK5	At2g33580	I + II + III	几丁质	LysM-RLK-II LYR	否 (Pseudo kinase)	正常
AtLYP1 (CEBiP-like1, LYM2)	At2g17120	* + VI + VII	几丁质	LYP	-	正常	C末端包含GPI锚定信号; OsCEBiP直系同源
AtLYP2 (CEBiP-like2, LYM1)	At1g21880	* + VI + VIII	肽聚糖	LYP	-	正常	C末端包含GPI锚定信号; 与OsLYP4和OsLYP6直系同源
AtLYP3 (CEBiP-like3, LYM3)	At1g77630	* + VI + VIII	肽聚糖	LYP	-	正常	C末端包含GPI锚定信号; 与OsLYP4和OsLYP6直系同源

图1 拟南芥和水稻中CERK1受体复合物及可能的信号激发机制(改自Macho and Zipfel, 2014) (A) 几丁质诱导AtCERK1同源二聚化和激活, 在此过程中BIK1对于NADPH氧化酶产生活性氧爆发是必需的; (B) 水稻中的几丁质受体复合物由OsCEBiP、OsCERK1和一些相关的胞质蛋白组成; 受几丁质刺激后, OsCERK1磷酸化OsRLCK185, 后者从受体复合物上解离下来并参与下游信号转导; 另外, OsCERK1激活OsRacGEF1/OsRac1, 从而引起活性氧爆发。CERK1: 几丁质激发子受体激酶; CEBiP: 几丁质激发子结合蛋白; BIK1: 葡萄胞属诱导激酶1; GEF1: 鸟嘌呤交换因子1; RLCK185: 细胞质类受体激酶185

Figure 1 CERK1 receptor complexes and signaling mechanism in Arabidopsis and rice (modified from Macho and Zipfel, 2014)(A) Chitin induces AtCERK1 homo-dimerization and activation, while BIK1 is required for subsequent reactive oxygen species burst from NADPH oxidase (RBOHD) during this process; (B) The chitin receptor complex is composed of OsCEBiP, OsCERK1 and several associated cytoplasmic proteins in rice; Upon stimuli from chitin, OsCERK1 phosphorylates OsRLCK185, which is released from the receptor complex and contributes to down-streaming signal transduction; Additionally, OsCERK1 activates the OsRacGEF1/OsRac1, which contributes to chitin-induced ROS burst. CERK1: Chitin elicitor receptor kinase1; CEBiP: Chitin elicitor binding protein; BIK1: Botrytis-induced kinase1; GEF1: Guanine exchange factor1; RLCK185: Receptor-like cytoplasmic kinase185

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LysM蛋白介导植物免疫防卫反应及其信号激发的研究进展

Studies of Innate Immunity Mediated by Lysin Motif Protein and Its Signaling Priming

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