基于形态和分子证据对斑果藤族和节蒴木科的系统学研究

doi:10.11983/CBB14152

植物学报 ›› 2015, Vol. 50 ›› Issue (4): 473-481.DOI: 10.11983/CBB14152

基于形态和分子证据对斑果藤族和节蒴木科的系统学研究

赵慧玲^1,², 鲁丽敏², 苏俊霞^1,^*(), 陈之端²

¹山西师范大学生命科学学院, 临汾 041004
²中国科学院植物研究所系统与进化植物学国家重点实验室, 北京 100093

收稿日期:2014-08-21 接受日期:2015-01-27 出版日期:2015-07-01 发布日期:2015-05-07
通讯作者: 苏俊霞
作者简介:
? 共同第一作者
基金资助:
国家自然科学基金(No.31300180, No.31270268)、国家重点基础研究发展计划(No.2014CB954100)、中国科学院海外科教基地建设计划(No.SAJC201315)、中国科学院国际合作局对外合作重点项目(No.GJIIZ201321)和山西师范大学校自然科学基金(No.ZR1312)

Phylogeny of Stixeae and Borthwickiaceae Based on Morphological and Molecular Data

Huiling Zhao^1,2, Limin Lu², Junxia Su^1*, Zhiduan Chen²

¹School of Life Sciences, Shanxi Normal University, Linfen 041004, China
²State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2014-08-21 Accepted:2015-01-27 Online:2015-07-01 Published:2015-05-07
Contact: Su Junxia
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 斑果藤族(Stixeae)和节蒴木属(Borthwickia)传统上被置于山柑科(Capparaceae), 最近的分子和形态学证据均支持它们从山柑科中分离出来, 节蒴木属已被提升为科, 但斑果藤族的系统位置和分类地位尚不清楚。为解决斑果藤族的系统位置和分类地位, 并进一步为节蒴木科的成立提供证据, 对核心十字花目GRFT (Gyrostemonaceae-Resedaceae- Forchhammeria-Tirania)分支, 使用叶表皮等20个形态性状和5个DNA片段(matK、rbcL、ndhF、trnL-F和ITS), 用最大简约法(MP)和贝叶斯分析(BI)对形态及分子矩阵进行系统发育分析。结果高度支持斑果藤族的Neothorelia为GRFT分支成员; Neothorelia、Tirania和斑果藤属(Stixis)聚为一支, 但Forchhammeria的位置尚不明确; 形态及分子系统发育分析结果均支持节蒴木科成立。

Abstract: Stixeae (including Stixis, Tirania, Neothorelia and Forchhammeria) and Borthwickia have traditionally been placed in Capparaceae. Recent molecular and morphological studies suggest Borthwickia as a new monotypic family. However, the positions and taxonomic ranks of Stixeae remain unresolved. To estimate the relationships of Stixeae, we reconstructed the phylogeny of the Gyrostemonaceae-Resedaceae-Forchhammeria-Tirania (GRFT) clade of the core Brassicales using maximum parsimony and Bayesian analysis with 20 morphological characters and five DNA regions (matK, rbcL, ndhF, trnL-F, and ITS). Our results strongly support Neothorelia as a new member of the GRFT clade; Neothorelia, Tirania and Stixis form a clade with strong support. The systematic position of Forchhammeria is still uncertain; molecular and morphological evidence support Borthwickiaceae as a new monotypic family.

赵慧玲, 鲁丽敏, 苏俊霞, 陈之端. 基于形态和分子证据对斑果藤族和节蒴木科的系统学研究. 植物学报, 2015, 50(4): 473-481.

Huiling Zhao, Limin Lu, Junxia Su, Zhiduan Chen. Phylogeny of Stixeae and Borthwickiaceae Based on Morphological and Molecular Data. Chinese Bulletin of Botany, 2015, 50(4): 473-481.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB14152

https://www.chinbullbotany.com/CN/Y2015/V50/I4/473

图/表 7

参考文献 34

1	洪亚平, 潘开玉, 陈之端, 路安民 (2001). 防己科植物的叶表皮特征及其系统学意义. 植物学报 43, 615-623.
2	桑涛, 徐炳声 (1996). 分支系统学当前的理论和方法概述及华东地区山胡椒属十二个种的分支系统学研究. 植物分类学报 34, 12-28.
3	苏俊霞 (2012). 蔷薇分支的分子系统学研究. 博士学位论文. 北京: 中国科学院植物研究所.
4	孙必兴 (1999). 山柑科. 见: 吴征镒编. 中国植物志(第32卷). 北京: 科学出版社. pp. 484-540.
5	孙稚颖, 张宪春 (2009). 中国瓦韦属药用植物的叶表皮观察. 植物学报 44, 331-337.
6	吴征镒, 路安民, 汤彦承, 陈之端, 李德铢 (2004). 中国被子植物科属综论. 北京: 科学出版社.
7	APG III (Angiosperm Phylogeny Group III). (2009). An update of the angiosperm phylogeny group classification for the orders and families of flowering plants: APG III.Bot J Linn Soc 161, 105-121.
8	Carlquist S, Hansen BF, Iltis HH, Olson ME, Geiger DL (2014). Forchhammeria and Stixis (Brassicales): stem and wood anatomical diversity, ecological and phylogenetic significance.Aliso 31, 59-75.
9	Dogan Y, Mert H, Akcan K (2008). Anatomical studies of Reseda lutea (Resedaceae).Phytologia Balcan 14, 91-95.
10	Dowled A, Reveal JL (2008). New suprageneric names for vascular plants.Phytologia 90, 416-417.
11	Erdtman G (1952). Pollen Morphology and Plant Taxonomy. Angiosperms. Stockholm: Almquist and Wiksell.
12	Hansen BF (1977). A Monograph of Forchhammeria (Capparidaceae). Master Thesis Dissertation, University of Wisconsin, Madison, Wisconsin, USA.
13	Hall JC, Iltis HH, Sytsma KJ (2004). Molecular phylogenetics of core Brassicales, placement of orphan genera Emblingia, Forchhammeria, Tirania, and character evolution.Syst Bot 29, 654-669.
14	Hall JC, Sytsma KJ, Iltis HH (2002). Phylogeny of Capparaceae and Brassicaceae based on chloroplast sequence data.Am J Bot 89, 1826-1842.
15	Hillis DM, Bull JJ (1993). An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis.Syst Biol 42, 182-192.
16	Hutchinson J (1967). The genera of flowering plants (Angiospermae), Vol. 2. Dicotyledons. Oxford: Clarendon Press.
17	Jacobs M (1968). Borthwickia (Capparaceae) from Yunnan and in fruit.Blumea 16, 360.
18	Kers LE (2003). Capparaceae. In: Kubitzki K, Bayer C, eds. The Families and Genera of Vascular Plants, Vol. 5. Flowering Plants: Dicotyledons; Malvales, Capparales, and non-betalain Caryophyllales. Berlin: Springer. pp. 36-56.
19	Kubitzki K (2003). Resedaceae. In: Kubitzki K, Bayer C, eds. The Families and Genera of Vascular Plants, Vol. 5. Flowering Plants: Dicotyledons; Malvales, Capparales, and non-betalain Caryophyllales. Berlin: Springer. pp. 334-338.
20	Mabberley DJ (2008). Mabberley’s plant-book, third edn. Cambridge: Cambridge University Press.
21	Martín-Bravo S, Meimberg H, Luceño M, Märkl W, Valcárcel V, Bräuchler C, Vargas P, Heubl G (2007). Molecular systematics and biogeography of Resedaceae based on ITS and trnL-F sequences.Mol Phylogenet Evol 44, 1105-1120.
22	Mitra K (1975). Contribution to the pollen morphology of the family Capparaceae.Bull Bot Surv India 17, 7-31.
23	Morton CM, Karol KG, Chase MW (1997). Taxonomic affinities of Physena (Physenaceae) and Asteropeia (Theaceae).Bot Rev 63, 231-239.
24	Pax F, Hoffmann K (1936). Capparidaceae. In: Engler A, Prantl K, eds. Die natürlichen Pflanzenfamilien, Vol. 17b. Leipzig: Engelmann. pp. 146-233.
25	Ronquist F, Huelsenbeck JP (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models.Bioinformatics 19, 1572-1574.
26	Ronse De Craene LP, Haston E (2006). The systematic relationships of glucosinolate-producing plants and related families: a cladistic investigation based on morphological and molecular characters.Bot J Linn Soc 151, 453-494.
27	Silvio F (2010). Micromorphological observations on leaf and pollen of Capparis L. sect. Capparis (Capparaceae).Plant Biosyst 138, 125-134.
28	Smith WW (1911). Borthwickia, a new genera of Cappari- daceae.Trans Proc Bot Soc Edinburgh 24, 175-176.
29	Stanford AM, Harden M, Parks CR (2000). Phylogeny and biogeography of Juglans (Juglanaceae) based on matK and ITS sequence data. Am J Bot 87, 872-882.
30	Swofford DL (2003). PAUP: phylogenetic analysis using parsimony (and other methods), version 4.0b10. Sunderland, Massachusetts: Sinauer.
31	Su JX, Wang W, Zhang LB, Chen ZD (2012). Phylogenetic placement of two enigmatic genera, Borthwickia and Stixis, based on molecular and pollen data, and the description of a new family of Brassicales, Borthwicki- aceae.Taxon 61, 601-611.
32	Takhtajan A (1997). Diversity and Classification of Flowering Plants. New York: Columbia University Press.
33	White TJ, Bruns T, Lee S, Taylor J (1990). Amplifications and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, California: Academic Press. pp. 315-322.
34	Zhang ML, Tucker GC (2008). Capparaceae. In: Wu ZY, Raven PH, Hong DY, eds. Flora of China, Vol. 7. Beijing: Science Press; St. Louis: Missouri Botanical Garden. pp. 433-450.

Taxon	Locality	Voucher
Borthwickia trifoliata W.W. Smith	Mengla, Yunnan	RS002
Stixis suaveolens (Roxb.) Pierre	Mengla, Yunnan	RS012
Stixis ovata subsp. fasciculata (King) Jacobs	Hekou, Yunnan	RS019
Stixis scandens Lour.	Mengla, Yunnan	RS013

Taxon	Locality	Voucher
Borthwickia trifoliata W.W. Smith	Mengla, Yunnan	RS002
Stixis suaveolens (Roxb.) Pierre	Mengla, Yunnan	RS012
Stixis ovata subsp. fasciculata (King) Jacobs	Hekou, Yunnan	RS019
Stixis scandens Lour.	Mengla, Yunnan	RS013

	matK	ndhF	rbcL	trnL-F	ITS	Five loci data	Morphological data	Total evidence
Total length	1 320	923	1 270	1 122	689	5 012	20	5 032
Variable characters	306	180	126	159	381	1 032	20	1 266
Informative characters	212	145	90	89	326	760	20	780
Number of trees	11 415	12 780	10	12 045	288	32	210	20
Length of trees	398	235	163	192	949	1 410	56	1 756
Consistency index	0.884	0.872	0.853	0.911	0.624	0.859	0.875	0.876
Retention index	0.946	0.942	0.938	0.953	0.887	0.938	0.963	0.936
Rescaled consistency index	0.837	0.822	0.799	0.869	0.553	0.806	0.842	0.820

	matK	ndhF	rbcL	trnL-F	ITS	Five loci data	Morphological data	Total evidence
Total length	1 320	923	1 270	1 122	689	5 012	20	5 032
Variable characters	306	180	126	159	381	1 032	20	1 266
Informative characters	212	145	90	89	326	760	20	780
Number of trees	11 415	12 780	10	12 045	288	32	210	20
Length of trees	398	235	163	192	949	1 410	56	1 756
Consistency index	0.884	0.872	0.853	0.911	0.624	0.859	0.875	0.876
Retention index	0.946	0.942	0.938	0.953	0.887	0.938	0.963	0.936
Rescaled consistency index	0.837	0.822	0.799	0.869	0.553	0.806	0.842	0.820

Taxon	Voucher	ITS	matK	ndhF	rbcL	trnL-F
Borthwickia trifoliata 1 W.W. Smith	SJX036	KR936124*	JQ733089	JQ733101	JQ733114	JQ733127
Borthwickia trifoliata 2 W.W. Smith	Jin & al. YNET 381	KR936122*	JQ733091	JQ733103	JQ733116	JQ733129
Borthwickia trifoliata 3 W.W. Smith	RS002	KR936123*	JQ733090	JQ733102	JQ733115	JQ733128
Caylusea hexagyna (Forssk.) M.L. Green		DQ987228	FJ212207	-	FJ212220	DQ987069
Forchhammeria trifoliata Radlk.		-	AY483245	AY483259	AY483277	-
Forchhammeria sessilifolia Standl.		-	AY483243,	AY483257	AY483275	-
Forchhammeria sp.		-	AY483244	AY483258	AY483276	-
Forchhammeria watsonii Rose		-	AY483246	AY483260	AY483278	-
Forchhammeria pallida Liebm.		-	-	AY122381	AY483274	AY122437
Gyrostemon sp.		-	AY483236	AY483252	L22439	-
Gyrostemon tepperi (F. Muell. ex H. Walter) A.S. George		-	AY483237	AY483253	L22440	-
Gyrostemon thesioides (Hook. f.) A.S. George		DQ987075	FJ212199	-	FJ212210	DQ986975
Oligomeris linifolia (Vahl) J.F. Macbr.		DQ987162	AY483240	AY483255	AY483272	FJ212256
Pentadiplandra brazzeana Baill.		-	AY483239	AY483254	U38533	AY122463
Reseda complicata Bory		DQ987171	FJ212205	-	FJ212218	DQ987046
Reseda crystallina Webb & Berthel		DQ987089	FJ212200	-	FJ212212	FJ212283
Reseda lutea L.	M. Merello, J. Stone, M. Eristavi & M. Khutsishvili 2211	KR936125*	AY483241	AY122406	AY483273	AY122464
Reseda luteola L.		DQ987171	FJ212206	-	FJ212219	DQ987050
Sesamoides purpurascens (L.) G. López.		DQ987216	FJ212208	-	FJ212221	DQ987064
Stixis sauveolens 1 (Roxb.) Pierre	BW04	KR936112*	JQ733092	JQ733104	JQ733117	JQ733130
Stixis sauveolens 2 (Roxb.) Pierre	RS012	KR936113*	JQ733094	JQ733106	JQ733119	JQ733131
Stixis sauveolens 3 (Roxb.) Pierre	RS015	KR936114*	JQ733096	JQ733108	JQ733121	JQ733132
Stixis sauveolens 4 (Roxb.) Pierre	Chen Z.D. HN171	KR936115*	JQ733100	JQ733113	JQ733126	JQ733133
Stixis ovata subsp. fasciculata 1 (King) Jacobs	RS001	KR936117*	JQ733093	JQ733105	JQ733118	JQ733134
Stixis ovata subsp. fasciculata 2 (King) Jacobs	RS013	KR936116*	JQ733095	JQ733107	JQ733120	JQ733135
Stixis ovata subsp. fasciculata 3 (King) Jacobs	RS017	KR936120*	JQ733097	JQ733109	JQ733122	JQ733136
Stixis ovata subsp. fasciculata 4 (King) Jacobs	RS019	KR936121*	JQ733098	JQ733110	JQ733123	JQ733137
Stixis ovata subsp. fasciculata 5 (King) Jacobs	Yang Z.G. 008	KR936119*	-	JQ733112	JQ733125	JQ733139
Stixis scandens Lour.	Shui et al.12443	KR936118*	JQ733099	JQ733111	JQ733124	JQ733138
Tersonia cyathiflora (Fenzl) A.S. George ex J.W.		-	AY483238	AY122404	L22441	AY122462
Tirania purpurea Pierre		-	-	AY483261	AY483279	-

基于形态和分子证据对斑果藤族和节蒴木科的系统学研究

Phylogeny of Stixeae and Borthwickiaceae Based on Morphological and Molecular Data

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Taxon	The number of morphological characters
Taxon	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
Neothorelia laotica Gagnep.	0	0	2	0	?	?	?	1	?	0	0	0	0	0	0	?	0	0	0	?
Tirania purpurea Pierre	0	0	0	1	?	0	0	0	?	0	0	0	1	0	2	0	0	?	0	1
Stixis sauveolens 1 (Roxb.) Pierre	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis sauveolens 2 (Roxb.) Pierre	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis sauveolens 3 (Roxb.) Pierre	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis sauveolens 4 (Roxb.) Pierre	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis ovata subsp. fasciculata 1 (King) Jacobs	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis ovata subsp. fasciculata 2 (King) Jacobs	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis ovata subsp. fasciculata 3 (King) Jacobs	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis ovata subsp. fasciculata 4 (King) Jacobs	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Stixis ovata subsp. fasciculata 5 (King) Jacobs	0	2	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	1	2
Stixis scandens Lour	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2
Borthwickia trifoliata 1 W.W. Smith	1	0/1	1	0	1	1	0	?	1	?	2	2	2	2	3	2	0	1	1	3
Borthwickia trifoliata 2 W.W. Smith	1	0/1	1	0	1	1	0	?	1	?	2	2	2	2	3	2	0	1	1	3
Borthwickia trifoliata 3 W.W. Smith	1	0/1	1	0	1	1	0	?	1	?	2	2	2	2	3	2	0	1	1	3
Reseda complicata Bory	1	3	0	1	?	1	0	0	0	1	3	1	1	0	4	3	1	2	2	4
Reseda luteola L.	1	3	0	1	?	1	0	0	0	1	3	1	1	0	4	3	1	2	2	4
Reseda crystallina Webb & Berthel.	1	3	0	1	?	1	0	0	0	1	3	1	1	0	4	3	1	2	2	4
Caylusea hexagyna (Forssk.) M.L. Green	1	3	0	1	?	1	0	0	0	1	2	2	1	3	4	3	1	2	2	4
Sesamoides purpurascens (L.) G. López.	1	4	0	1	?	1	0	0	0	1	2	1	1	?	4	3	1	?	2	4
Oligomeris linifolia (Vahl) J.F. Macbr.	1	3	0	1	?	1	0	0	0	1	2	3	1	2	4	3	1	0	2	4
Reseda lutea L.	1	3	0	1	?	1	0	0	0	1	3	1	1	0	4	3	1	2	2	4
Gyrostemon sp.	0	0	0	1	?	0	1	0	0	1	1	1	1	4	1	0	0	3	2	6
Gyrostemon tepperi (F. Muell. ex H. Walter) A.S. George	0	0	0	1	?	0	1	0	0	1	1	1	1	4	1	0	0	3	2	6
Gyrostemon thesioides (Hook. f.) A.S. George	0	0	0	1	?	0	1	0	0	1	1	1	1	4	1	0	0	3	2	6
Forchhammeria trifoliata Radlk.	0	1	0	0	?	?	1	0	0	?	1	1	0	1	1	1	0	0	0	0
Forchhammeria sessilifolia Standl.	0	1	0	0	?	?	1	0	0	?	1	1	0	1	1	1	0	0	0	0
Forchhammeria sp.	0	1	0	0	?	?	1	0	0	?	1	1	0	1	1	1	0	0	0	0
Forchhammeria watsonii Rose	0	1	0	0	?	?	1	0	0	?	1	1	0	1	1	1	0	0	0	0
Forchhammeria pallida Liebm.	0	1	0	0	?	?	1	0	0	?	1	1	0	1	1	1	0	0	0	0
Pentadiplandra brazzeana Baill.	0	0	0	1	?	0	1	0	?	0	4	5	1	?	?	?	?	0	2	1
Tersonia cyathiflora (Fenzl) A.S. George ex J.W.	0	0	0	1	?	0	1	0	0	1	1	1	1	4	1	0	0	3	2	6