Chin Bull Bot ›› 2017, Vol. 52 ›› Issue (2): 188-201.doi: 10.11983/CBB16054

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Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii

Zheng Jun1, Qiao Ling1, Zhao Jiajia1, Qiao Linyi2, Zhang Shichang2, Chang Jianzhong2, Tang Caiguo3,*(), Yang Sanwei1,*()   

  1. 1Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen 041000, China
    2Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
    3Hefei Institutes of Physical Science, Institute of Technical Biology & Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, China
  • Received:2016-03-18 Accepted:2016-08-04 Online:2017-04-05 Published:2017-03-01
  • Contact: Tang Caiguo,Yang Sanwei E-mail:tcgpinder@126.com;ysvysv@163.com
  • About author:# Co-first authors

Abstract:

The control of flowering time is a crucial environmental adaptation in plants; numerous CCT domain genes control flowering in plants. Bioinformatics was used for a genome-wide research of CCT domain genes in Aegilops tauschii. In this study, 26 CCT domain genes were identified in A. tauschii, distributed on seven chromosomes of the A. tauschii genome. The predicted molecular weight of this family was spread over 14.9 to 83.2 kDa, and 25 proteins contain a complete CCT conserved structure domain. Twelve pairs of A. tauschii-Triticum urartu and 9 pairs of A. tauschii-rice CCT proteins were orthologous in the phylogenetic tree. Specific expression and constitutive expression were found in the CCT gene family; nine AetCCT genes were constitutively expressed in all organisms, including AetCCT3, AetCCT4, AetCCT7 and AetCCT9; AetCCT15, AetCCT21 and AetCCT25 were specifically expressed in leaf, seed and roots of A. tauschii, respectively. Moreover, the members responded to phytohormone treatments differently, which suggested a complex function and characteristic in metabolism of this family. Light conditions affect the expression of AetCCT, and this gene family is regulated by photoperiod and vernalization. The results of this paper not only provide useful information for wheat evolution studies, but also provide theory basis for comprehensive understanding of formation and interaction characteristics of important traits.

Key words: Aegilops tauschii, CCT gene family, sequence analysis, responses to phytohormones

Figure 1

Chromosome mapping and QTL distribution of CCT genes in Aegilops tauschii genomeGenetic scale is indicated on the left side of chromosomes, the heading date QTLs are indicated on the right side of the chromosomes in black."

Figure 2

Intron-exon structures of CCT genes in Aegilops tauschiiExons are represented by bars, introns are represented by connecting lines."

Figure 3

Motif distribution of AetCCT proteins in Aegilops tauschii"

Figure 4

Motifs of CCT proteins in Aegilops tauschii"

Figure 5

Phylogenesis of CCT proteinsCCT proteins of Triticum urartu and Aegilops tauschii were indicated by triangles and circles, respectively."

Figure 6

Cis-elements in CCT genes promoters in response to phytohormones of Aegilops tauschii"

Table 1

CCT gene family in Aegilops tauschii"

Gene Length (bp) CDS Scaffold Location Mapped seq. Chromosome
AetCCT1 2211 AEGTA18365 Scaffold4528 49937-52147 AT1D0317 1DL
AetCCT2 1836 AEGTA21580 Scaffold71509 42038-43873 AT1D0523 1DL
AetCCT3 1333 AEGTA27323 Scaffold97182 45576-47039 AT1D0969 1DL
AetCCT4 3096 AEGTA10532 Scaffold38896 10923-14018 AT2D1124 2DS
AetCCT5 2241 AEGTA13911 Scaffold30755 94901-97141 AT2D1581 2DS
AetCCT6 2157 AEGTA00203 Scaffold30755 129179-131557 AT2D1581 2DS
AetCCT7 2226 AEGTA31460 Scaffold77907 6990-9215 AT3D2607 3DS
AetCCT8 1134 AEGTA06337 Scaffold4745 59908-61041 AT3D3130 3DL
AetCCT9 2692 AEGTA28548 Scaffold28581 76532-79223 AT4D3632 4DS
AetCCT10 606 AEGTA01709 Scaffold50727 43106-43711 AT4D3683 4DS
AetCCT11 3208 AEGTA21446 Scaffold108 140572-143968 AT4D3728 4DL
AetCCT12 4521 AEGTA31746 Scaffold2864 19243-23723 AT4D3886 4DL
AetCCT13 2234 AEGTA18304 Scaffold98624 23875-26108 BE403305 4DL
AetCCT14 2878 AEGTA13770 Scaffold12030 253420-256287 AT4D4194 4DL
AetCCT15 603 AEGTA33063 Scaffold24714 5731-6333 AT5D4519 5DL
AetCCT16 1059 AEGTA03508 Scaffold185863 19051-20109 AT5D4590 5DL
AetCCT17 2404 AEGTA04421 Scaffold53469 30253-32656 AT5D5030 5DL
AetCCT18 738 AEGTA32221 Scaffold7166 12609-13346 AT5D5201 5DL
AetCCT19 3597 AEGTA05461 Scaffold106936 10967-14734 AT6D5284 6DS
AetCCT20 1188 AEGTA21198 Scaffold137524 2620-3965 AT6D5798 6DL
AetCCT21 2031 AEGTA15475 Scaffold71269 16605-18635 AT7D6397 7DS
AetCCT22 2781 AEGTA31079 Scaffold66553 48279-51059 AT7D6416 7DS
AetCCT23 3075 AEGTA08066 Scaffold6305 52570-55644 AT7D6716 7DS
AetCCT24 1521 AEGTA13638 Scaffold7100 34283-35803 AT7D7002 7DL
AetCCT25 742 AEGTA03492 Scaffold116052 16157-16929 AT7D7027 7DL
AetCCT26 2036 AEGTA22574 Scaffold16211 9228-11710 AT7D7167 7DL

Figure 7

Expression profile of CCT genes in different tissues and developmental stages of Aegilops tauschii"

Figure 8

Hierarchical clustering of CCT domain genes under different phytohormones treatments in Aegilops tauschii(A) Phytohormones treatment for 24 h; (B) Phytohormones treatment for 72 h"

Figure 9

Expression of CCT domain genes under different light treatments in Aegilops tauschiiNV-LD: Long-day condition without vernalization (16 h light/8 h dark); NV-SD: Short-day condition without vernalization (8 h light/16 h dark); LD: Long-day condition after vernalization (16 h light/8 h dark); SD: Short-day condition after vernalization (8 h light/16 h dark)."

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