植物学报 ›› 2020, Vol. 55 ›› Issue (1): 76-82.DOI: 10.11983/CBB19208

• 技术方法 • 上一篇    下一篇

植物蛋白磷酸化的检测方法

朱丹,曹汉威,李媛,任东涛()   

  1. 中国农业大学生物学院, 植物生理学与生物化学国家重点实验室, 北京 100193
  • 收稿日期:2019-10-24 接受日期:2019-12-31 出版日期:2020-01-01 发布日期:2020-01-03
  • 通讯作者: 任东涛
  • 基金资助:
    国家自然科学基金(No.31670248);国家自然科学基金(No.31970276)

Protocols for Analyzing Plant Phospho-proteins

Dan Zhu,Hanwei Cao,Yuan Li,Dongtao Ren()   

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
  • Received:2019-10-24 Accepted:2019-12-31 Online:2020-01-01 Published:2020-01-03
  • Contact: Dongtao Ren

摘要:

蛋白磷酸化是一种重要的蛋白质翻译后修饰方式, 几乎参与植物所有生命过程的调节。蛋白磷酸化过程主要指在蛋白激酶的催化作用下, 将三磷酸腺苷(ATP)上的γ位磷酸基团转移到底物蛋白特定氨基酸残基上的过程。底物蛋白上被磷酸化的常见氨基酸有丝氨酸、苏氨酸及酪氨酸, 磷酸基团与氨基酸中的羟基通过酯键连接。该文详细描述了几种常用的蛋白质体外及体内磷酸化的检测方法及注意事项。

关键词: 蛋白磷酸化, 蛋白激酶, 植物

Abstract:

Protein phosphorylation is one of the important protein posttranslational modifications that is involved in the regulation of most cellular processes in plants. Protein kinases catalyze the phosphorylation by transferring the phosphate group in ATP to the substrate proteins. The phosphate is usually covalently linked to the hydroxyl group of specific amino acid residues in the substrates by an ester bond. The mostly studied phosphorylation sites are serine, threonine, and tyrosine residues. Here, we present protocols and related tips for the in vitro and in vivo protein phosphorylation assays.

Key words: protein phosphorylation, protein kinase, plant