氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响

doi:10.11983/CBB16033

植物学报 ›› 2017, Vol. 52 ›› Issue (2): 218-224.DOI: 10.11983/CBB16033

氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响

陈曼, 涂艺声^*(), 叶丽婻, 杨碧芸

江西师范大学生命科学学院, 南昌 330022

收稿日期:2016-02-24 接受日期:2016-09-04 出版日期:2017-03-01 发布日期:2017-04-05
通讯作者: 涂艺声
作者简介:
# 共同第一作者
基金资助:
国家自然科学基金(No.81360614, No.31370390, No.81660597)、江西省自然科学基金(No.20132BAB204023)和江西省科技支撑项目(No.20121BBG70016)

Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata

Man Chen, YishengTu^*, Linan Ye, Biyun Yang

College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China

Received:2016-02-24 Accepted:2016-09-04 Online:2017-03-01 Published:2017-04-05
Contact: YishengTu
About author:
# Co-first authors

摘要/Abstract

摘要： 通过在培养基中添加不同浓度的天冬氨酸、赖氨酸和色氨酸, 探究其对蛇足石杉(Huperzia serrata)叶状体生长、石杉碱甲积累及SOD酶活性的影响。结果表明: 添加天冬氨酸后, 叶状体干重生物量比对照提高18.50%-25.75%, 石杉碱甲含量亦明显高于对照, 其最适天冬氨酸浓度为0.5 mmol·L^-1; 添加1 mmol·L^-1色氨酸后, 叶状体相对增殖率显著高于对照, 但其石杉碱甲积累量略低于对照; 添加赖氨酸后, 叶状体相对增殖量、干重生物量及石杉碱甲含量均低于对照。添加一定浓度的天冬氨酸使SOD酶活性适当提高, 同时叶状体石杉碱甲积累量增加, 而长期保持高水平的SOD酶活性对叶状体积累石杉碱甲不利。

Abstract: We investigated the effects of amino acid supplements on thallus growth, superoxide dismutase (SOD) activity and huperzine-A accumulation in Huperzia serrata by adding aspartic acid, lysine, and tryptophan at different concentrations into the medium. With aspartic acid, the dry weight of the thallus increased by 18.50%-25.75% and the huperzine-A accumulation was significantly higher than in the control. The optimal concentration of aspartic acid was 0.5 mmol·L^-1. The relative growth of thallus was significantly higher with 1 mmol·L^-1tryptophan than in the control, but the huperzine-A accumulation was slightly lower than for the control. The relative growth rate, dry weight and huperzine-A accumulation was lower for thallus cultured with lysine than the control. The SOD activity of the thallus cultured with aspartic acid was increased properly, and the huperzine-A accumulation of the thallus was increased. However, huperzine-A accumulation of the thallus was suppressed when SOD activity remained high in the long-term.

陈曼, 涂艺声, 叶丽婻, 杨碧芸. 氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响. 植物学报, 2017, 52(2): 218-224.

Man Chen, YishengTu, Linan Ye, Biyun Yang. Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata. Chinese Bulletin of Botany, 2017, 52(2): 218-224.

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https://www.chinbullbotany.com/CN/Y2017/V52/I2/218

图/表 5

图1 石杉碱甲标准品(A)及蛇足石杉离体叶状体培养物提取物(B)的HPLC (高效液相色谱)色谱图

Figure 1 The HPLC (high performance liquid chromatography fluorescence) test of huperzine A standard (A) and extract of Huperzia serrata thallus in vitro culture (B)

图2 添加不同氨基酸培养的蛇足石杉叶状体(A) 添加天冬氨酸(Asp)培养的蛇足石杉叶状体; (B) 添加色氨酸(Trp)培养的蛇足石杉叶状体; (C) 添加赖氨酸(Lys)培养的蛇足石杉叶状体

Figure 2 The thallus of Huperzia serrata supplemented with different amino acids(A) The thallus of H. serrata supplemented with Asp; (B) The thallus of H. serrata supplemented with Trp; (C) The thallus of H. serrata supplemented with Lys

表1 添加不同浓度的天冬氨酸(Asp)、赖氨酸(Lys)和色氨酸(Trp)的培养基对蛇足石杉叶状体生物量的影响

Table 1 Effect of different concentrations of Asp, Lys or Trp on the biomass of thallus in Huperzia serrata

Concentration of different amino acid (mmol·L^-1)	Relative growth rate (%)	Dry weight (g·L^-1)
Water/control	1313.05±21 bc	7.61680
Asp 0.25	1189.68±19 bc	9.30778
Asp 0.50	1337.76±29 bc	9.02623
Asp 1.00	1217.20±25 bc	9.57869
Asp 2.00	1211.28±32 bc	7.92295
Lys 0.25	1108.18±27 c	7.57992
Lys 0.50	1222.61±31 bc	7.40164
Lys 1.00	1328.25±30 bc	7.58278
Lys 2.00	1231.90±35 bc	7.32459
Trp 0.25	1311.53±33 bc	7.80328
Trp 0.50	1460.42±24 ab	8.01024
Trp 1.00	1647.51±37 a	9.35860
Trp 2.00	1050.53±17 c	6.66844

图3 添加不同浓度的天冬氨酸(Asp)、赖氨酸(Lys)和色氨酸(Trp)对蛇足石杉叶状体中石杉碱甲含量的影响

Figure 3 Effect of different concentrations of Asp, Lys and Trp on the content of huperzine A in thallus of Huperzia serrata

图4 添加不同氨基酸培养蛇足石杉叶状体的SOD酶活性特征(A) 添加0.5 mmol·L-1天冬氨酸(Asp)和0.25 mmol·L-1赖氨酸(Lys)培养叶状体SOD酶活性变化特征, (B) 添加不同浓度Asp培养50天叶状体SOD酶活性特征

Figure 4 The change features of SOD activity for Huperzia serrata thallus supplemented with different amino acids(A) The change features of SOD activity for H. serrata thallus supplemented with 0.5 mmol·L-1 Asp and 0.25 mmol·L-1 Lys, respectively; (B) The SOD activity of H. serrata thallus supplemented with different concentrations of Asp on 50 days

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氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响

Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata

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