植物学报 ›› 2016, Vol. 51 ›› Issue (5): 684-690.doi: 10.11983/CBB16008

• 技术方法 • 上一篇    下一篇

海三棱藨草的组织培养与快繁体系

张群1,2,, 吕秀立1,, 何小丽1, 朱义1, 崔心红1,*()   

  1. 1上海市园林科学规划研究院, 上海 200232
    2复旦大学生物多样性科学研究所, 生物多样性和生态工程教育部重点实验室, 上海 200438
  • 收稿日期:2016-01-13 接受日期:2016-05-12 出版日期:2016-09-01 发布日期:2016-09-27
  • 通讯作者: 张群,吕秀立,崔心红 E-mail:kysxinhongcui@163.com
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    上海市科学技术委员会科技创新行动计划(No.14DZ1206002)

A Rapid Propagation System for Scirpus × mariqueter

Qun Zhang1,2†, Xiuli Lü1†, Xiaoli He1, Yi Zhu1, Xinhong Cui1*   

  1. 1Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
    2Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Sciences, Fudan University, Shanghai 200438
  • Received:2016-01-13 Accepted:2016-05-12 Online:2016-09-01 Published:2016-09-27
  • Contact: Zhang Qun,Lü Xiuli,Cui Xinhong E-mail:kysxinhongcui@163.com
  • About author:

    # Co-first authors

摘要:

以海三棱藨草(Scirpus × mariqueter)成熟种子为外植体, 通过无菌萌发、丛生芽诱导、增殖、壮苗、生根和移栽等过程, 建立了海三棱藨草的无菌快繁体系。结果表明: 丛生芽诱导和增殖的最适培养基为MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; 壮苗最适培养基为1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; 生根最适培养基为1/2 MS+0.2 mg·L-1 IBA; 最适培养温度为30°C; 再生苗移入珍珠岩:草炭:蛭石=1:1:1 (体积比)的混合基质中, 移栽成活率可达85%以上。生根培养阶段选用容积较大的塑料容器育苗, 可以降低生产成本和提高生产效率。

Abstract:

The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L-1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.

表1

经过不同处理的海三棱藨草种子在M1培养基上的萌发情况"

Treatment No. of contamination No. of aseptic seedlings Percentage of germination (%)
Intact seed 1.00±1.00 5.33 5.33b
Piercing naked seed 26.00±2.65 15.00 15.00a
Naked seed 38.00±3.00 4.00 4.00c

图1

海三棱藨草种子诱导植株再生 (A) 部分去种皮的种胚(左), 完全去种皮的种胚(右); (B) 无菌芽; (C) 丛生苗; (D) 丛生苗矮化团状; (E) 低温下逐渐枯死的丛生苗; (F) 高温叶片焦灼; (G) 生根; (H) 塑料容器培养的幼苗; (I) 移栽"

表2

植物生长调节剂对海三棱藨草诱导增殖的影响"

No. of medium Phytohormone composition (mg·L-1) Multiplication
coefficient
Differentiation status and color change of leaves
6-BA TDZ IBA
M2 0.1 0 0.1 1.00d No differentiation, yellow, weak and thin
M3 0.5 0 0.1 1.00d No differentiation, yellow, weak and thin
M4 1.0 0 0.1 1.17d Few differentiation, yellow-green, weak and thin
M5 2.0 0 0.2 1.13d Few differentiation, weak and thin and died
M6 1.0 0.002 0.1 1.42c Green, weak and thin, multiple shoots were induced
M7 1.0 0.002 0.2 1.50c Green, weak and thin, multiple shoots were induced
M8 2.0 0.002 0.2 2.68a Green, weak and thin, multiple shoots were induced
M9 2.0 0.002 0.3 2.53a Green, weak and thin, multiple shoots were induced
M10 3.0 0.002 0.3 2.02b Green, weak and thin, multiple shoots were induced

表4

植物生长调节剂对海三棱藨草丛生芽壮苗的影响"

No. of
medium
Phytohormone composition (mg·L-1) Multiplication
coefficient
Height
(cm)
Differentiation status and color change of leaves
6-BA IBA
M11 0 0 1.12d 3-4 Few yellow-green, some rooting shoots
M12 0.05 0.01 1.42cd 3-4 Thriving, multiple shoots uniform
M13 0.1 0.01 1.67bc 2-3 Relatively uniform, few small buds
M14 0.2 0.02 1.83b 2-3 Multiple shoots short, some small buds
M15 0.5 0.05 3.02a 1-2 Multiple shoots short, more small buds

表5

植物生长调节剂对海三棱藨草丛生芽生根的影响"

No. of
medium
Phytohormone composition (mg·L-1) Percentage of
rooting shoots (%)
Description of root growing and differentiation status
IBA NAA
M16 0.05 0 83.33ab Roots relatively uniform and strongly
M17 0.1 0 96.67a Roots relatively uniform and strongly
M18 0.2 0 100.00a Roots uniform and strongly
M19 0.5 0 100.00a Roots uniform and too much
M20 0 0.2 66.67b Roots not consistent, long and thin, callus were induced
M21 0.2 0.2 100.00a Roots not consistent, long and thin, callus were induced

表3

培养温度对海三棱藨草增殖的影响"

Culture temperature (°C) Multiplication coefficient Differentiation status and color change of leaves
23 1.87d Yellow-green and died
25 2.68c Green, weak and thin
28 2.92abc Green
30 3.25ab Dark green, thriving
32 3.18a Dark green, few burned by high temperature

表6

不同培养容器对海三棱藨草接种效率的影响"

Type of
container
Inoculation
speed (plant·h-1)
Inoculation density
(plant·container-1)
Percentage of
contamination (%)
Description of root growing
Plastic case 198.67 10 5.04a Grew strongly and good condition
Glass bottle 134.33 25 5.27a Grew strongly and good condition

表7

不同介质以及不同种植密度对海三棱藨草移栽成活的影响"

Treatment Medium and ratio (v/v) Density (plant·hole-1) No. of seedlings Survival rate (%)
1 Perlite:turf=2:1 1 60 31.67h
2 Perlite:turf=2:1 2 60 36.11gh
3 Perlite:turf=1:1 1 60 43.33fg
4 Perlite:turf=1:1 2 60 47.78ef
5 Perlite:turf=1:2 1 60 76.67b
6 Perlite:turf=1:2 2 60 69.44b
7 Perlite:turf:vermiculite=1:1:1 1 60 88.89a
8 Perlite:turf:vermiculite=1:1:1 2 60 87.22a
9 Perlite:Chongming fresh soil=1:1 1 60 58.33cd
10 Perlite:Chongming fresh soil=1:1 2 60 60.00cd
11 Chongming fresh soil 1 60 52.78de
12 Chongming fresh soil 2 60 61.67c
1 陈建林 (2003). 荸荠组培苗高效扩繁技术的研究. 硕士论文. 扬州: 扬州大学. pp. 1-6.
2 陈中义 (2004). 互花米草入侵国际重要湿地崇明东滩的生态后果. 博士论文. 上海: 复旦大学. pp. 2-8.
3 陈中义 (2005). 长江口海三棱藨草的生态价值及利用与保护. 河南科技大学学报(自然科学版) 26(2) , 64-67.
4 杜雪玲, 张振霞, 余如刚, 符义坤 (2005). 植物组织培养中的污染成因及其预防. 草业科学 22, 24-27.
5 何松林, 孔德政, 杨秋生, 孟中生, 王美茹, 吴建华 (2003). 组织培养容器环境因子调控技术研究进展. 河南农业大学学报 37, 25-32.
6 黄华梅 (2009). 上海滩涂盐沼植被的分布格局和时空动态研究. 博士论文. 上海: 华东师范大学. pp. 27-39.
7 李华 (2009). 潮间带盐沼植物的沉积动力学效应研究. 博士论文. 上海: 华东师范大学. pp. 3-28.
8 欧尚华, 方永鑫, 周根余 (1992). 海三棱藨草种子萌发条件的初步研究. 上海师范大学学报(自然科学版) 21(增刊), 23-26.
9 童春富, 章飞军, 陆健健 (2007). 长江口海三棱藨草带生长季大型底栖动物群落变化特征. 动物学研究 28, 640-646.
10 徐晓峰, 黄学林 (2003). TDZ: 一种有效的植物生长调节剂. 植物学通报 20, 227-237.
11 杨梅 (2010). 海三棱藨草的物种生物学和遗传结构研究. 博士论文. 上海: 复旦大学. pp. 8-10.
12 赵雨云, 马志军, 陈家宽 (2002). 崇明东滩越冬白头鹤食性的研究. 复旦学报(自然科学版) 41, 609-613.
13 周博, 许维倩, 李娜, 徐娜, 姜长阳 (2010). 银线伞莎草组织培养及快速繁殖的研究. 北方园艺 17, 150-152.
14 朱晶, 敬凯, 干晓静, 马志军 (2007). 迁徙停歇期鸻鹬类在崇明东滩潮间带的食物分布. 生态学报 27, 2149-2159.
15 Chalupa V (1987). Effect of benzylaminopurine and thidiazuron on in vitro shoot proliferation of Tilia cordata Mill, Sorbus aucuparia L. and Robinia pseudoacacia L.Biol Plantarum 29, 425-429.
16 Chen H, Wang DQ, Chen ZL, Wang J, Xu SY (2005). The variation of sediments organic carbon content in Chong- ming east tidal flat during Scirpus mariqueter growing stage.J Geogr Sci 15, 500-508.
17 Gan XJ, Cai YT, Choi CY, Ma ZJ, Chen JK, Li B (2009). Potential impacts of invasive Spartina alterniflora on spring bird communities at Chongming Dongtan, a Chi- nese wetland of international importance.Estuar Coast Shelf S 83, 211-218.
18 Li B, Liao CH, Zhang XD, Chen HL, Wang Q, Chen ZY, Gan XJ, Wu JH, Zhao B, Ma ZJ, Cheng XL, Jiang LF, Chen JK (2009). Spartina alterniflora invasions in the Yangtze River Estuary, China: an overview of current status and ecosystem effects.Ecol Eng 35, 511-520.
19 Liao CZ, Peng RH, Luo YQ, Zhou XH, Wu XW, Fang CM, Chen JK, Li B (2008). Altered ecosystem carbon and nitrogen cycles by plant invasion: a meta-analysis.New Phytol 177, 706-714.
20 Peng RH, Fang CM, Li B, Chen JK (2011). Spartina al- terniflora invasion increases soil inorganic nitrogen pools through interactions with tidal subsidies in the Yangtze Estuary, China.Oecologia 165, 797-807.
21 Quan WM, Fu CZ, Jin BS, Luo YQ, Li B, Chen JK, Wu JH (2007). Tidal marshes as energy sources for commercially important nektonic organisms: stable isotope analysis.Mar Ecol Prog Ser 352, 89-99.
22 Wang M, Chen JK, Li B (2007). Characterization of bacterial community structure and diversity in rhizosphere soils of three plants in rapidly changing salt marshes using 16S rDNA.Pedosphere 17, 545-556.
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