植物学报 ›› 2016, Vol. 51 ›› Issue (5): 684-690.DOI: 10.11983/CBB16008

• 技术方法 • 上一篇    下一篇

海三棱藨草的组织培养与快繁体系

张群1,2,, 吕秀立1,, 何小丽1, 朱义1, 崔心红1,*()   

  1. 1上海市园林科学规划研究院, 上海 200232
    2复旦大学生物多样性科学研究所, 生物多样性和生态工程教育部重点实验室, 上海 200438
  • 收稿日期:2016-01-13 接受日期:2016-05-12 出版日期:2016-09-01 发布日期:2016-09-27
  • 通讯作者: 张群,吕秀立,崔心红
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    上海市科学技术委员会科技创新行动计划(No.14DZ1206002)

A Rapid Propagation System for Scirpus × mariqueter

Qun Zhang1,2†, Xiuli Lü1†, Xiaoli He1, Yi Zhu1, Xinhong Cui1*   

  1. 1Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
    2Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Sciences, Fudan University, Shanghai 200438
  • Received:2016-01-13 Accepted:2016-05-12 Online:2016-09-01 Published:2016-09-27
  • Contact: Zhang Qun,Lü Xiuli,Cui Xinhong
  • About author:

    # Co-first authors

摘要:

以海三棱藨草(Scirpus × mariqueter)成熟种子为外植体, 通过无菌萌发、丛生芽诱导、增殖、壮苗、生根和移栽等过程, 建立了海三棱藨草的无菌快繁体系。结果表明: 丛生芽诱导和增殖的最适培养基为MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; 壮苗最适培养基为1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; 生根最适培养基为1/2 MS+0.2 mg·L-1 IBA; 最适培养温度为30°C; 再生苗移入珍珠岩:草炭:蛭石=1:1:1 (体积比)的混合基质中, 移栽成活率可达85%以上。生根培养阶段选用容积较大的塑料容器育苗, 可以降低生产成本和提高生产效率。

Abstract:

The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L-1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.