海三棱藨草的组织培养与快繁体系

doi:10.11983/CBB16008

植物学报 ›› 2016, Vol. 51 ›› Issue (5): 684-690.DOI: 10.11983/CBB16008

海三棱藨草的组织培养与快繁体系

张群^1,^2,^†, 吕秀立^1,^†, 何小丽¹, 朱义¹, 崔心红^1,^*()

¹上海市园林科学规划研究院, 上海 200232
²复旦大学生物多样性科学研究所, 生物多样性和生态工程教育部重点实验室, 上海 200438

收稿日期:2016-01-13 接受日期:2016-05-12 出版日期:2016-09-01 发布日期:2016-09-27
通讯作者: 张群,吕秀立,崔心红
作者简介:
# 共同第一作者
基金资助:
上海市科学技术委员会科技创新行动计划(No.14DZ1206002)

A Rapid Propagation System for Scirpus × mariqueter

Qun Zhang^1,2†, Xiuli Lü^1†, Xiaoli He¹, Yi Zhu¹, Xinhong Cui^1*

¹Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
²Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Sciences, Fudan University, Shanghai 200438

Received:2016-01-13 Accepted:2016-05-12 Online:2016-09-01 Published:2016-09-27
Contact: Zhang Qun,Lü Xiuli,Cui Xinhong
About author:
# Co-first authors

摘要/Abstract

摘要： 以海三棱藨草(Scirpus × mariqueter)成熟种子为外植体, 通过无菌萌发、丛生芽诱导、增殖、壮苗、生根和移栽等过程, 建立了海三棱藨草的无菌快繁体系。结果表明: 丛生芽诱导和增殖的最适培养基为MS+2.0 mg·L^-16-BA+0.002 mg·L^-1 TDZ+0.2 mg·L^-1 IBA; 壮苗最适培养基为1/2MS+0.05 mg·L^-16-BA+0.01 mg·L^-1 IBA; 生根最适培养基为1/2 MS+0.2 mg·L^-1 IBA; 最适培养温度为30°C; 再生苗移入珍珠岩:草炭:蛭石=1:1:1 (体积比)的混合基质中, 移栽成活率可达85%以上。生根培养阶段选用容积较大的塑料容器育苗, 可以降低生产成本和提高生产效率。

Abstract: The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L^-16-BA+0.002 mg·L^-1TDZ+0.2 mg·L^-1IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L^-16-BA+0.01 mg·L^-1IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L^-1IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.

张群, 吕秀立, 何小丽, 朱义, 崔心红. 海三棱藨草的组织培养与快繁体系. 植物学报, 2016, 51(5): 684-690.

Qun Zhang, Xiuli Lü, Xiaoli He, Yi Zhu, Xinhong Cui. A Rapid Propagation System for Scirpus × mariqueter. Chinese Bulletin of Botany, 2016, 51(5): 684-690.

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参考文献 22

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Treatment	No. of contamination	No. of aseptic seedlings	Percentage of germination (%)
Intact seed	1.00±1.00	5.33	5.33^b
Piercing naked seed	26.00±2.65	15.00	15.00^a
Naked seed	38.00±3.00	4.00	4.00^c

Treatment	No. of contamination	No. of aseptic seedlings	Percentage of germination (%)
Intact seed	1.00±1.00	5.33	5.33^b
Piercing naked seed	26.00±2.65	15.00	15.00^a
Naked seed	38.00±3.00	4.00	4.00^c

No. of medium	Phytohormone composition (mg·L^-1)			Multiplication coefficient	Differentiation status and color change of leaves
No. of medium	6-BA	TDZ	IBA	Multiplication coefficient	Differentiation status and color change of leaves
M2	0.1	0	0.1	1.00^d	No differentiation, yellow, weak and thin
M3	0.5	0	0.1	1.00^d	No differentiation, yellow, weak and thin
M4	1.0	0	0.1	1.17^d	Few differentiation, yellow-green, weak and thin
M5	2.0	0	0.2	1.13^d	Few differentiation, weak and thin and died
M6	1.0	0.002	0.1	1.42^c	Green, weak and thin, multiple shoots were induced
M7	1.0	0.002	0.2	1.50^c	Green, weak and thin, multiple shoots were induced
M8	2.0	0.002	0.2	2.68^a	Green, weak and thin, multiple shoots were induced
M9	2.0	0.002	0.3	2.53^a	Green, weak and thin, multiple shoots were induced
M10	3.0	0.002	0.3	2.02^b	Green, weak and thin, multiple shoots were induced

No. of medium	Phytohormone composition (mg·L^-1)			Multiplication coefficient	Differentiation status and color change of leaves
No. of medium	6-BA	TDZ	IBA	Multiplication coefficient	Differentiation status and color change of leaves
M2	0.1	0	0.1	1.00^d	No differentiation, yellow, weak and thin
M3	0.5	0	0.1	1.00^d	No differentiation, yellow, weak and thin
M4	1.0	0	0.1	1.17^d	Few differentiation, yellow-green, weak and thin
M5	2.0	0	0.2	1.13^d	Few differentiation, weak and thin and died
M6	1.0	0.002	0.1	1.42^c	Green, weak and thin, multiple shoots were induced
M7	1.0	0.002	0.2	1.50^c	Green, weak and thin, multiple shoots were induced
M8	2.0	0.002	0.2	2.68^a	Green, weak and thin, multiple shoots were induced
M9	2.0	0.002	0.3	2.53^a	Green, weak and thin, multiple shoots were induced
M10	3.0	0.002	0.3	2.02^b	Green, weak and thin, multiple shoots were induced

No. of medium	Phytohormone composition (mg·L^-1)		Multiplication coefficient	Height (cm)	Differentiation status and color change of leaves
No. of medium	6-BA	IBA	Multiplication coefficient	Height (cm)	Differentiation status and color change of leaves
M11	0	0	1.12^d	3-4	Few yellow-green, some rooting shoots
M12	0.05	0.01	1.42^cd	3-4	Thriving, multiple shoots uniform
M13	0.1	0.01	1.67^bc	2-3	Relatively uniform, few small buds
M14	0.2	0.02	1.83^b	2-3	Multiple shoots short, some small buds
M15	0.5	0.05	3.02^a	1-2	Multiple shoots short, more small buds

海三棱藨草的组织培养与快繁体系

A Rapid Propagation System for Scirpus × mariqueter

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No. of medium	Phytohormone composition (mg·L^-1)		Percentage of rooting shoots (%)	Description of root growing and differentiation status
No. of medium	IBA	NAA	Percentage of rooting shoots (%)	Description of root growing and differentiation status
M16	0.05	0	83.33^ab	Roots relatively uniform and strongly
M17	0.1	0	96.67^a	Roots relatively uniform and strongly
M18	0.2	0	100.00^a	Roots uniform and strongly
M19	0.5	0	100.00^a	Roots uniform and too much
M20	0	0.2	66.67^b	Roots not consistent, long and thin, callus were induced
M21	0.2	0.2	100.00^a	Roots not consistent, long and thin, callus were induced

Culture temperature (°C)	Multiplication coefficient	Differentiation status and color change of leaves
23	1.87^d	Yellow-green and died
25	2.68^c	Green, weak and thin
28	2.92^abc	Green
30	3.25^ab	Dark green, thriving
32	3.18^a	Dark green, few burned by high temperature

Type of container	Inoculation speed (plant·h^-1)	Inoculation density (plant·container^-1)	Percentage of contamination (%)	Description of root growing
Plastic case	198.67	10	5.04^a	Grew strongly and good condition
Glass bottle	134.33	25	5.27^a	Grew strongly and good condition

Treatment	Medium and ratio (v/v)	Density (plant·hole^-1)	No. of seedlings	Survival rate (%)
1	Perlite:turf=2:1	1	60	31.67^h
2	Perlite:turf=2:1	2	60	36.11^gh
3	Perlite:turf=1:1	1	60	43.33^fg
4	Perlite:turf=1:1	2	60	47.78^ef
5	Perlite:turf=1:2	1	60	76.67^b
6	Perlite:turf=1:2	2	60	69.44^b
7	Perlite:turf:vermiculite=1:1:1	1	60	88.89^a
8	Perlite:turf:vermiculite=1:1:1	2	60	87.22^a
9	Perlite:Chongming fresh soil=1:1	1	60	58.33^cd
10	Perlite:Chongming fresh soil=1:1	2	60	60.00^cd
11	Chongming fresh soil	1	60	52.78^de
12	Chongming fresh soil	2	60	61.67^c