一种优化的测定水稻硅含量的方法

doi:10.11983/CBB15181

植物学报 ›› 2016, Vol. 51 ›› Issue (5): 679-683.DOI: 10.11983/CBB15181 cstr: 32102.14.CBB15181

一种优化的测定水稻硅含量的方法

贾雨薇^1,^†, 杨瑞林^2,^†, 张洋¹, 房娟娟¹, 陈惠^1,^*()

¹山西师范大学生命科学学院, 临汾 041004
²山西师范大学分析测试中心, 临汾 041004

收稿日期:2015-10-10 接受日期:2016-02-14 出版日期:2016-09-01 发布日期:2016-09-27
通讯作者: 贾雨薇,杨瑞林,陈惠
作者简介:
# 共同第一作者
基金资助:
国家自然科学基金(No.30170236)和山西省基础研究项目(No.2012011032)

An Optimized Method to Determine Silicon Content in Rice

Yuwei Jia^1†, Ruilin Yang^2†, Yang Zhang¹, Juanjuan Fang¹, Hui Chen^1*

¹College of Life Sciences, Shanxi Normal University, Linfen 041004, China
²Center of Analysis and Test, Shanxi Normal University, Linfen 041004, China

Received:2015-10-10 Accepted:2016-02-14 Online:2016-09-01 Published:2016-09-27
Contact: Jia Yuwei,Yang Ruilin,Chen Hui
About author:
# Co-first authors

摘要/Abstract

摘要： 为建立一种更为简便且准确的水稻(Oryza sativa)硅含量测定方法, 对硅钼蓝分光光度法的多个实验条件进行了优化, 并对水稻样品的前处理——消解法和高压灭菌法进行了比较研究。结果表明, 还原剂为抗坏血酸、测定波长为600 nm、硅钼黄显色时间和硅钼蓝稳定时间分别为5和25分钟为最佳测定条件, 样品的前处理则是消解法优于高压灭菌法, 前者提取硅的效果好于后者。该优化方法为深入研究水稻和其它植物中硅的吸收和转运机制奠定了基础。

null

Abstract: To establish an easier and more accurate method to determine silicon content in rice, several main parameters of silicon molybdenum-blue spectrophotometry were optimized in combination with 2 sample pretreatments, digestion and autoclaving. By using ascorbic acid as a reductant, with the color development time of the silicon molybdenum yellow and the stable time of silicon molybdenum blue for 5 and 25 min, respectively, optimal silicon content in rice was achieved at 600 nm wavelength by molybdenum-blue spectrophotometry. For sample pretreatment, the digestion method was more effective for silicon extraction than autoclaving. Briefly, the optimized method facilitates the determination of silicon content in rice and other plants and could lay a theoretical foundation for further research of the mechanism of silicon absorbance and transportation.

贾雨薇, 杨瑞林, 张洋, 房娟娟, 陈惠. 一种优化的测定水稻硅含量的方法. 植物学报, 2016, 51(5): 679-683.

Yuwei Jia, Ruilin Yang, Yang Zhang, Juanjuan Fang, Hui Chen. An Optimized Method to Determine Silicon Content in Rice. Chinese Bulletin of Botany, 2016, 51(5): 679-683.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB15181

https://www.chinbullbotany.com/CN/Y2016/V51/I5/679

图/表 3

图1 硅钼蓝分光光度法条件的优化及两种前处理样品方法测得硅含量的比较 (A) 3种还原剂(A+B液、抗坏血酸和硫酸亚铁铵)的全波长扫描图; (B) 硅钼蓝稳定时间(平均值±标准差, n=9); (C) 硅标准曲线; (D) 消解法与高压灭菌法处理水稻样品后所测硅含量的比较(平均值±标准差, n=9)。不同大写字母表示同一水稻样品不同方法之间差异显著(P<0.05, n=9); 不同小写字母表示同一方法不同样品之间差异显著(P<0.05, n=9)。

Figure 1 Optimization of conditions of silicon molybdenum blue spectrophotometric and comparison between two methods of samples pretreatment for determination of silicon content (A) The full wavelength scan figure of three reducing agents (A+B liquid, ascorbic acid, and ammonium iron (II) sulfate); (B) Stable time of silicon molybdenum blue (means±SD, n=9); (C) Silicon standard curve; (D) Comparison of the silicon content in rice samples by using digestion and autoclaving method (means±SD, n=9). Different capital letters represent significant difference across the same samples at different methods (P<0.05, n=9); Different lowercase letters represent significant difference across the same methods at different samples (P<0.05, n=9).

表1 600和812 nm波长测定的比较

Table 1 Comparison of measurement using absorbance at the 600 and 812 nm wavelength

Wavelength (nm)	Observation number	Minimum OD value	Maximum OD value	OD value (means±SD)
600	9	0.540	0.557	0.5468±0.006686
812	9	1.621	1.646	1.6302±0.011904

表2 硅钼黄显色时间分析

Table 2 Analysis of color development time of silicon molybdenum yellow

Group	Observation number	Minimum OD value	Maximum OD value	OD value (means±SD)
Boiling water bath 30 s	9	0.529	0.532	0.5308±0.0011 c
Standing 5 min	9	0.534	0.536	0.5348±0.0007 a
Standing 10 min	9	0.534	0.536	0.5348±0.0008 a
Standing 15 min	9	0.532	0.535	0.5333±0.0013 ab
Standing 20 min	9	0.528	0.535	0.5321±0.0030 b
Standing 30 min	9	0.528	0.535	0.5320±0.0031 b

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一种优化的测定水稻硅含量的方法

An Optimized Method to Determine Silicon Content in Rice

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