Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (2): 308-315.DOI: 10.11983/CBB22184

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

An Efficient System for Regenerating Adventitious Buds in Stem Segments of Tea Plants

Lulu Ren1, Youze Zhang1, Kelin Huang1, Xiaochun Wan1, Zhaoliang Zhang1,*(), Mulan Zhu2,3,*(), Chaoling Wei1,*()   

  1. 1Key Laboratory of Tea Biology and Tea Processing, Ministry of Agriculture and Rural Areas, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
    2Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences, Shanghai 200032, China
    3Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China
  • Received:2022-08-03 Accepted:2022-11-15 Online:2023-03-01 Published:2023-03-15
  • Contact: *E-mail:;;

Abstract: Tea plant is an important economic crop with high heterozygosity and variation. There are few reports on the in vitro regeneration system with high efficiency. In this study, the stem segment of a tea cultivar Shuchazao was used as the initial explants to identify the factors which could efficiently influence the regeneration of adventitious buds. The results showed that MS+2 mg∙L-1 6-BA was the best formula for normal buds induction, and the induction rate was 84.44%. The expansion rate of the absorption chassis was 80%, which was beneficial to the subsequent induction of adventitious buds. MS+2 mg∙L-1 6-BA+0.2 mg∙L-1 NAA+0.1 mg∙L-1 KT+1 mg∙L-1 proline was the suitable formula for the induction of adventitious buds, and the induction rate was 88.89%. The average number of shoots was 7.8. 1/2MS+3 mg∙L-1 IBA was the suitable formula for adventitious root induction, and the rooting rate was 85.56%. The regenerated plants were subjected to molecular verification by RAPD and ISSR techniques. The results showed that no significant variation was found in the two consecutive generations of regenerated plants in vitro.

Key words: Camellia sinensis, in vitro regeneration, adventitious bud induction, genetic stability