Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (2): 285-297.DOI: 10.11983/CBB22034

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

A Plant Sample Optimal Pretreatment for Flow Cytometric Analysis

Jindan Zhang, Min Feng()   

  1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2022-02-23 Accepted:2022-09-07 Online:2023-03-01 Published:2023-03-15
  • Contact: *E-mail:

Abstract: Flow cytometry requires preparation of suspensions of intact nuclei, which is crucial step for analysis. A newly formulated buffer, PVPK12-mGB2 compared with two common buffers (LB01 and CyStain® PI Absolute P), were used to isolate nuclei from fresh or silica gel desiccated leaf tissues of different species. PVPK12-mGB2 exhibited the best performance on all fresh leaves tested of plant species, of which the interfering secondary metabolites highly accumulated, the quality of extracted nuclei were able to satisfy demands for flow cytometric analysis. Moreover, results of the present study substantiate the enhanced effectiveness of PVPK12-mGB2, for silica gel desiccated plant tissue, compared to other buffers tested. The study established an optimal pretreatment of plants for DNA flow cytometry, which provided reference method for sampling and analysis in remote regions.

Key words: nuclear isolation buffer, silica gel desiccated, interfering secondary metabolites, flow cytometry