Photosynthetic and Physiological Regulation of C4 Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) by Exogenous Ca2+ Under Polyethylene Glycol Stress

doi:10.3724/SP.J.1259.2015.00206

Abstract

Abstract: Phosphoenolpyruvate carboxylase (PEPC), a key C₄ photosynthetic enzyme, participates in photosynthesis by fixing CO₂ in C₄ plants. In this study, we aimed to understand the drought-tolerance mechanism of C₄ PEPC transgenic rice with high photosynthetic efficiency. C₄ PEPC transgenic rice plants (PC) and wild-type rice plants (WT) at the 4-5 leaf age were used. Using 15% polyethylene glycol-6000 (PEG) treatment for different days, we investigated leaf relative water content (RWC), net photosynthetic rate (P_n), protein kinase activity, PEPCase activity and PEPC gene expression in leaves of rice plants with different concentrations of CaCl₂. RWC of PC leaf was enhanced with 0.5 mmol∙L^-1CaCl₂ (P<0.05) except under 2 and 10 mmol∙L^-1CaCl₂, and factors in the WT were unaffected by exogenous CaCl₂. P_n of PC was not affected by CaCl₂ treatments combined with 15% PEG. Moreover, PC showed an certain capacity for maintenance and stabilization of endogenous Ca²⁺ level in leaves under PEG treatment: with higher exogenous Ca²⁺ treatment (10 mmol∙L^-1), PEPCase activity, PEPC expression and soluble protein were all decreased in PC leaves.

Key words: Ca2+, C4 PEPC transgenic rice, drought stress, PEPCase, photosynthetic characteristics, rice (Oryza sativa)

Xiaolong Liu, Xia Li, Baoyun Qian. Photosynthetic and Physiological Regulation of C₄ Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) by Exogenous Ca²⁺ Under Polyethylene Glycol Stress[J]. Chinese Bulletin of Botany, 2015, 50(2): 206-216.

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Photosynthetic and Physiological Regulation of C₄ Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) by Exogenous Ca²⁺ Under Polyethylene Glycol Stress

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