Photosynthetic and Physiological Regulation of C4 Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) by Exogenous Ca2+ Under Polyethylene Glycol Stress

doi:10.3724/SP.J.1259.2015.00206

Abstract

Abstract: Phosphoenolpyruvate carboxylase (PEPC), a key C₄ photosynthetic enzyme, participates in photosynthesis by fixing CO₂ in C₄ plants. In this study, we aimed to understand the drought-tolerance mechanism of C₄ PEPC transgenic rice with high photosynthetic efficiency. C₄ PEPC transgenic rice plants (PC) and wild-type rice plants (WT) at the 4-5 leaf age were used. Using 15% polyethylene glycol-6000 (PEG) treatment for different days, we investigated leaf relative water content (RWC), net photosynthetic rate (P_n), protein kinase activity, PEPCase activity and PEPC gene expression in leaves of rice plants with different concentrations of CaCl₂. RWC of PC leaf was enhanced with 0.5 mmol∙L^-1CaCl₂ (P<0.05) except under 2 and 10 mmol∙L^-1CaCl₂, and factors in the WT were unaffected by exogenous CaCl₂. P_n of PC was not affected by CaCl₂ treatments combined with 15% PEG. Moreover, PC showed an certain capacity for maintenance and stabilization of endogenous Ca²⁺ level in leaves under PEG treatment: with higher exogenous Ca²⁺ treatment (10 mmol∙L^-1), PEPCase activity, PEPC expression and soluble protein were all decreased in PC leaves.

Key words: Ca2+, C4 PEPC transgenic rice, drought stress, PEPCase, photosynthetic characteristics, rice (Oryza sativa)

Xiaolong Liu, Xia Li, Baoyun Qian. Photosynthetic and Physiological Regulation of C₄ Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) by Exogenous Ca²⁺ Under Polyethylene Glycol Stress[J]. Chinese Bulletin of Botany, 2015, 50(2): 206-216.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2015.00206

https://www.chinbullbotany.com/EN/Y2015/V50/I2/206

References

1 陈平波, 李霞 (2012). 低浓度NO对高表达转玉米C 4 型 PEPC 水稻光合的促进. 植物研究 32, 402-409.
2 丁在松, 周宝元, 孙雪芳, 赵明 (2012). 干旱胁迫下 PEPC 过表达增强水稻的耐强光能力. 作物学报 38, 285-292.
3 董秀梅, 晁青, 王柏臣 (2013). 植物磷酸烯醇式丙酮酸羧激酶(PEPCK)研究进展. 植物学报 48, 320-328.
4 姬飞腾, 李楠, 邓馨 (2009). 喀斯特地区植物钙含量特征与高钙适应方式分析. 植物生态学报 33, 926-935.
5 蒋明敏, 徐晟, 夏冰, 彭峰, 汪仁 (2012). 干旱胁迫下外源氯化钙、水杨酸和一氧化氮对石蒜抗旱性的影响. 植物生理学报 48, 909-916.
6 焦德茂, 匡廷云, 李霞, 戈巧英, 黄雪清, 郝乃斌, 白克智 (2003). 转 PEPC 基因水稻具有初级CO 2 浓缩机制生理特点. 中国科学(C辑) 33, 33-39.
7 李青云, 葛会波, 胡淑明, 王惠英 (2006). 钠盐和钙盐胁迫对草莓光合作用的影响. 西北植物学报 26, 1713-1717.
8 李霞, 焦德茂, 戴传超 (2005). 转 PEPC 基因水稻对光氧化逆境的响应. 作物学报 31, 408-413.
9 李霞, 吴爽, 焦德茂, 王守海, 李成荃, 古森本 (2001). 转 PEPC 基因水稻的选育. 江苏农业学报 17, 143-147.
10 杨彩琴, 刘伟娜, 赵志弘, 吴海燕 (1998). 血清钙的甲基百里香酚蓝测定法. 光谱学与光谱分析 18, 485-487.
11 周宝元, 丁在松, 赵明 (2011). PEPC过表达可以减轻干旱胁迫对水稻光合的抑制作用. 作物学报 37, 112-118.
12 Alleva K, Niemietz CM, Sutka M, Maurel C, Parisi M, Tyerman SD, Amodeo G (2006). Plasma membrane of Beta vulgaris storage root shows high water channel activity regulated by cytoplasmic pH and a dual range of calcium concentrations. J Exp Bot 57, 609-621.
13 Anjum SA, Wang LC, Farooq M, Hussain M, Xue LL, Zou CM (2011). Brassinolide application improves the drought tolerance in maize through modulation of enzymatic antioxidants and leaf gas exchange. J Agron Crop Sci 197, 177-185.
14 Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248- 254.
15 Chaves MM, Flexas J, Pinheiro C (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103, 551-560.
16 Chen PB, Li X, Huo K, Wei XD, Dai CC, Lv CG (2014). Promotion of photosynthesis in transgenic rice over- expressing of maize C 4 phosphoenolpyruvate carboxylase gene by nitric oxide donors. J Plant Physiol 171, 458-466.
17 Cousins AB, Baroli I, Badger MR, Ivakov A, Lea PJ, Leegood RC, von Caemmerer S (2007). The role of phosphoenolpyruvate carboxylase during C 4 photosyn- thetic isotope exchange and stomatal conductance. Plant Physiol 145, 1006-1017.
18 Dodd AN, Kudla J, Sanders D (2010). The language of calcium signaling. Annu Rev Plant Biol 61, 593-620.
19 Fu YL, Zhang GB, Lv XF, Guan Y, Yi HY, Gong JM (2013). Arabidopsis histone methylase CAU1/PRMT5/SKB1 acts as an epigenetic suppressor of the calcium signaling gene CAS to mediate stomatal closure in response to extracellular calcium. Plant Cell 25, 2878-2891.
20 Fukayama H, Hatch MD, Tamai T, Tsuchida H, Sudoh S, Furbank RT, Miyao M (2003). Activity regulation and physiological impacts of maize C 4 -specific phosphoenolpyruvate carboxylase overproduced in transgenic rice plants. Photosynth Res 77, 227-239.
21 Gerbeau P, Amodeo G, Henzler T, Santoni V, Ripoche P, Maurel C (2002). The water permeability of Arabidopsis plasma membrane is regulated by divalent cations and pH. Plant J 30, 71-81.
22 Giglioli-Guivarc'h N, Pierre JN, Brown S, Chollet R, Vidal J, Gadal P (1996). The light-dependent transduction pathway controlling the regulatory phosphorylation of C 4 phosphoenolpyruvate carboxylase in protoplasts from digitaria sanguinalis. Plant Cell 8, 573-586.
23 Hartwell J, Gill A, Nimmo GA, Wilkins MB, Jenkins GI, Nimmo HG (1999). Phosphoenolpyruvate carboxylase kinase is a novel protein kinase regulated at the level of expression. Plant J 20, 333-342.
24 Hirschi KD (2004). The calcium conundrum. Both versatile nutrient and specific signal. Plant Physiol 136, 2438- 2442.
25 Jeanneau M, Gerentes D, Foueillassar X, Zivy M, Vidal J, Toppan A, Perez P (2002). Improvement of drought tole- rance in maize: towards the functional validation of the Zm-Asr1 gene and increase of water use efficiency by over-expressing C 4 -PEPC. Biochimie 84, 1127-1135.
26 Karki S, Rizal G, Quick WP (2013). Improvement of photosynthesis in rice ( Oryza sativa L.) by inserting the C 4 pathway. Rice 6, 28.
27 Ku MSB, Agarie S, Nomura M, Fukayama H, Tsuchida H, Ono K, Hirose S, Toki S, Miyao M, Matsuoka M (1999). High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants. Nat Biotechnol 17, 76-80.
28 Li X, Cao K, Wang C, Sun ZW, Yan LN (2010). Variation of photosynthetic tolerance of rice cultivars ( Oryza sativa L.) to chilling temperature in the light. Afr J Biotech 9, 1325-1337.
29 Li X, Wang C, Ren CG (2011). Effects of 1-butanol, neomy- cin, and calcium on the photosynthetic characteristics of pepc transgenic rice. Afr J Biotech 10, 17466-17476.
30 Lindberg S, Kader A, Yemelyanov V (2012). Calcium signaling in plant cells under environmental stress. In: Ahmad P, Prasad MNV, eds. Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change. New York: Springer. pp. 325-360.
31 Monreal JA, Arias-Baldrich C, Pérez-Montaño F, Gandullo J, Echevarría C, García-Mauriño S (2013a). Factors involved in the rise of phosphoenolpyruvate carboxylase-kinase activity caused by salinity in sorghum leaves. Planta 237, 1401-1413.
32 Monreal JA, Arias-Baldrich C, Tossi V, Feria AB, Rubio- Casal A, García-Mata C, Lamattina L, García-Mauriño S (2013b). Nitric oxide regulation of leaf phosphoenolpyruvate carboxylase-kinase activity: implication in sorghum responses to salinity. Planta 238, 859-869.
33 Ren CG, Li X, Liu XL, Wei XD, Dai CC (2014). Hydrogen peroxide regulated photosynthesis in C 4 - PEPC transgenic rice. Plant Physiol Biochem 74, 218-229.
34 Schägger H (2006). Tricine-SDS-PAGE. Nat Protoc 1, 16-22.
35 Silva JV, de Lacerda CF, da Costa PHA, Enéas-Filho J, Gomes-Filho E, Prisco JT (2003). Physiological res- ponses of NaCl stressed cowpea plants grown in nutrient solution supplemented with CaCl 2 . Braz J Plant Physiol 15, 99-105.
36 Vidal J, Chollet R (1997). Regulatory phosphorylation of C 4 PEP carboxylase. Trends Plant Sci 2, 230-237.
37 Wang WH, Yi XQ, Han AD, Liu TW, Chen J, Wu FH, Dong XJ, He JX, Pei ZM, Zheng HL (2012). Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response to extracellular calcium in Arabidopsis. J Exp Bot 63, 177-190.
38 White PJ, Broadley MR (2003). Calcium in plants. Ann Bot 92, 487-511.
39 Wu Y, Liu XF, Wang WF, Zhang SQ, Xu BC (2012). Calcium regulates the cell-to-cell water flow pathway in maize roots during variable water conditions. Plant Physiol Biochem 58, 212-219.
40 Xu C, Li X, Zhang L (2013). The effect of calcium chloride on growth, photosynthesis, and antioxidant responses of Zoysia japonica under drought conditions. PLoS One 8, e68214.
41 Xue S, Hu H, Ries A, Merilo E, Kollist H, Schroeder JI (2011). Central functions of bicarbonate in S-type anion channel activation and OST1 protein kinase in CO 2 signal transduction in guard cell. EMBO J 30, 1645-1658.
42 Zhang S, Klessig DF (1997). Salicylic acid activates a 48 kDa MAP kinase in tobacco. Plant Cell 9, 809-824.
43 Zhang SQ, Liu YD (2001). Activation of salicylic acid- induced protein kinase, a mitogen-activated protein kinase, induces multiple defense responses in tobacco. Plant Cell 13, 1877-1889.
44 Zhu GH, Kurek I, Liu L (2010). Engineering photosynthetic enzymes involved in CO 2 -assimilation by gene shuffling. In: Rebeiz CA, Benning C, Bohnert HJ, Daniell H, Hoober JK, Lichtenthaler HK, Portis AR, Tripathy BC, eds. The Chloroplast: Basics and Applications. Netherlands: Sprin- ger. pp. 307-322.

[1]	Zheng Jiang Dingqin Liu Yuxuan Miao Lingyan Zhou Ying Feng Jianing Pei Ruiqiang Liu Xuhui Zhou Yu-Ling Fu. Effects of drought on plant root exudates and associated rhizosphere priming effect: review and prospect [J]. Chin J Plant Ecol, 2024, 48(7): 0-0.
[2]	Laipeng Zhao, Baike Wang, Tao Yang, Ning Li, Haitao Yang, Juan Wang, Huizhuan Yan. Investigation of SlHVA22l gene regulating drought tolerance in tomato [J]. Chinese Bulletin of Botany, 2024, 59(4): 0-0.
[3]	FAN Hong-Kun, ZENG Tao, JIN Guang-Ze, LIU Zhi-Li. Leaf trait variation and trade-offs among growth types of broadleaf plants in Xiao Hinggan Mountains [J]. Chin J Plant Ecol, 2024, 48(3): 364-376.
[4]	Zhang Yingchuan, Wu Xiaomingyu, Tao Baolong, Chen Li, Lu Haiqin, Zhao Lun, Wen Jing, Yi Bin, Tu Jinxing, Fu Tingdong, Shen Jinxiong. Bna-miR43 Mediates the Response of Drought Tolerance in Brassica napus [J]. Chinese Bulletin of Botany, 2023, 58(5): 701-711.
[5]	CHEN Tu-Qiang, XU Gui-Qing, LIU Shen-Si, LI Yan. Hydraulic traits adjustments and nonstructural carbohydrate dynamics of Haloxylon ammodendron under drought stress [J]. Chin J Plant Ecol, 2023, 47(10): 1407-1421.
[6]	LI Bian-Bian, ZHANG Feng-Hua, ZHAO Ya-Guang, SUN Bing-Nan. Effects of different clipping degrees on non-structural carbohydrate metabolism and biomass of Cyperus esculentus [J]. Chin J Plant Ecol, 2023, 47(1): 101-113.
[7]	ZHOU Jie, YANG Xiao-Dong, WANG Ya-Yun, LONG Yan-Xin, WANG Yan, LI Bo-Rui, SUN Qi-Xing, SUN Nan. Difference in adaptation strategy between Haloxylon ammodendron and Alhagi sparsifolia to drought [J]. Chin J Plant Ecol, 2022, 46(9): 1064-1076.
[8]	Yongmei Che, Yanjun Sun, Songchong Lu, Lixia Hou, Xinxin Fan, Xin Liu. AtMYB77 Involves in Lateral Root Development via Regulating Nitric Oxide Biosynthesis under Drought Stress in Arabidopsis thaliana [J]. Chinese Bulletin of Botany, 2021, 56(4): 404-413.
[9]	Jiaxin Li, Xia Li, Yinfeng Xie. Mechanism on Drought Tolerance Enhanced by Exogenous Trehalose in C₄-PEPC Rice [J]. Chinese Bulletin of Botany, 2021, 56(3): 296-314.
[10]	ZHANG Xiao-Yan, WEE Kim Shan Alison, KAJITA Tadashi, CAO Kun-Fang. Effects of provenance on leaf structure and function of two mangrove species: the genetic adaptation to temperature [J]. Chin J Plant Ecol, 2021, 45(11): 1241-1250.
[11]	Ningxi Song, Yingfeng Xie, Xia Li. Effects of Epigenetic Mechanisms on C₄ Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) Seed Germination Under Drought Stress [J]. Chinese Bulletin of Botany, 2020, 55(6): 677-692.
[12]	LIU Li-Yan, FENG Jin-Xia, LIU Wen-Xin, WAN Xian-Chong. Effects of drought stress on photosynthesis, growth and root structure of transgenic PtPIP2;8 poplar 84K (Populus alba × P. glandulosa) [J]. Chin J Plant Ecol, 2020, 44(6): 677-686.
[13]	LUO Jin-Huan, TAN Zhao-Yuan, CHEN Bin, CHEN Guang-Wu, JIANG Kai, HEI Qi-Fang, ZHANG Hui. Key characteristics for facilitating Leucaena leucocephala to successfully invade pioneer communities of tropical rain forests [J]. Chin J Plant Ecol, 2020, 44(12): 1215-1223.
[14]	Tong Zhang,Yalu Guo,Yue Chen,Jinjiao Ma,Jinping Lan,Gaowei Yan,Yuqing Liu,Shan Xu,Liyun Li,Guozhen Liu,Shijuan Dou. Expression Characterization of Rice OsPR10A and Its Function in Response to Drought Stress [J]. Chinese Bulletin of Botany, 2019, 54(6): 711-722.
[15]	Huaifeng Gao,Yafei Zhang,Guodong Wang,Xiwu Sun,Yue He,Futian Peng,Yuansong Xiao. The Effect of Molybdenum on Drought Stress Response in Peach [J]. Chinese Bulletin of Botany, 2019, 54(2): 227-236.

Photosynthetic and Physiological Regulation of C₄ Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) by Exogenous Ca²⁺ Under Polyethylene Glycol Stress

RichHTML

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments