Chinese Bulletin of Botany ›› 2010, Vol. 45 ›› Issue (01): 88-90.DOI: 10.3969/j.issn.1674-3466.2010.01.012
• 技术方法 • Previous Articles Next Articles
Guirong Qiao1, 2, Haiying Li1, Jing Jiang1, Zongxiu Sun2, Renying Zhuo1*
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Abstract:
Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L–1 NAA, 0.5 mg·L–1 6-BA, 15 mg·L–1 phenylacetic acid (PAA), 7.5 mg·L–1 silver thiosulfate (STS), 500 mg·L–1 casein enzymatic hydrolysate (CH), 100 mg·L–1 proline, 100 mg·L–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.
Guirong Qiao;Haiying Li;Jing Jiang;Zongxiu Sun;Renying Zhuo*. Anther Culture and Plant Regeneration of Dendrocalamus latiflorus[J]. Chinese Bulletin of Botany, 2010, 45(01): 88-90.
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https://www.chinbullbotany.com/EN/Y2010/V45/I01/88