Chinese Bulletin of Botany ›› 2010, Vol. 45 ›› Issue (01): 88-90.DOI: 10.3969/j.issn.1674-3466.2010.01.012  cstr: 32102.14.j.issn.1674-3466.2010.01.012

• 技术方法 • Previous Articles     Next Articles

Anther Culture and Plant Regeneration of Dendrocalamus latiflorus

Guirong Qiao1, 2, Haiying Li1, Jing Jiang1, Zongxiu Sun2, Renying Zhuo1*

  

  1. 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2009-01-21 Revised:2009-03-31 Online:2010-01-01 Published:2010-01-01
  • Contact: Renying Zhuo

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Abstract:

Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L–1 NAA, 0.5 mg·L–1 6-BA, 15 mg·L–1 phenylacetic acid (PAA), 7.5 mg·L–1 silver thiosulfate (STS), 500 mg·L–1 casein enzymatic hydrolysate (CH), 100 mg·L–1 proline, 100 mg·L–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.

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