Physiological and Molecular Response Mechanisms Under Low-temperature Stress in Lavandula angustifolia Leaves

doi:10.11983/CBB22046

Abstract

Abstract: The growth and reproduction as well as quality and yield of Lavandula angustifolia, a valuable aromatic plant, are severely affected by low temperatures. In our previous study, a low-temperature tolerant lavender variety was obtained. This study revealed the physiological and molecular regulatory mechanisms of L. angustifolia in response to cold stress. The relevant cold tolerance genes were mined and analyzed using transcriptomic and bioinformatics approaches. The feasibility of external application of salicylic acid to alleviate -10°C freezing stress was also explored. The results showed that seven genes encoding fatty acid desaturases and transferases (LaFADs), three genes involved in the synthesis of soluble sugars (LaBAM1, LaSS2), 19 genes encoding late embryonic abundant proteins (LaLEAs), and seven genes encoding peroxidases (LaPODs) were found to be up-regulated at low temperatures, directing L. angustifolia to synthesize and accumulate protective substances which maintained membrane stability in response to stress. In addition, salicylic acid pretreatment at 150 mg·L^-1was effective in alleviating freezing damage to plants and could be used as a low-temperature protectant. This study provides a basis for further understanding molecular mechanism of L. angustifolia responding to low-temperature stress.

Key words: Lavandula angustifolia, chilling injury, freezing injury, transcriptomic analysis, late embriogenesis abundant protein (LEA)

Lin Haijiao, Qu Jiaqi, Liu Yinan, Yuan Zening. Physiological and Molecular Response Mechanisms Under Low-temperature Stress in Lavandula angustifolia Leaves[J]. Chinese Bulletin of Botany, 2022, 57(5): 611-622.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22046

https://www.chinbullbotany.com/EN/Y2022/V57/I5/611

Figures/Tables 13

References 41

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Gene ID	Gene name	Primer sequence (5′-3′)
DN12834_c2_g2	LaLEA1	F: CCAAGGCAATCTCTGCTCTCA	R: CTTTGGATCCGGAGCCTTTCT
DN11187_c0_g4	LaLEA2	F: TCTACGTCTTCATCTTCGCCG	R: GCGTTGTAGTTTGCCAGAGTG
DN10094_c0_g1	LaERD10	F: ATATGACGACGCACCGACTG	R: TCCGGTGCGTAATCCGAATC
DN14123_c1_g1	LaFAB2	F: CGACTTTTGTCTCCCACGGA	R: TGTCCCATCTGGGTCGATCT
DN16955_c0_g1	LaPOD2	F: CATGGACTATGTCACGCCGA	R: GCTCGAAGAAAGCAATGGCA
DN18853_c0_g2	LaBAM1	F: CCCACGGCGAGAGAATTGTA	R: TTTGAGCGATGGGGACGTAG
	GADPH	F: TAGGAGGTGGCAGGACATCA	R: CCCTTTACCCGTCACGTTGT

Gene ID	Gene name	Primer sequence (5′-3′)
DN12834_c2_g2	LaLEA1	F: CCAAGGCAATCTCTGCTCTCA	R: CTTTGGATCCGGAGCCTTTCT
DN11187_c0_g4	LaLEA2	F: TCTACGTCTTCATCTTCGCCG	R: GCGTTGTAGTTTGCCAGAGTG
DN10094_c0_g1	LaERD10	F: ATATGACGACGCACCGACTG	R: TCCGGTGCGTAATCCGAATC
DN14123_c1_g1	LaFAB2	F: CGACTTTTGTCTCCCACGGA	R: TGTCCCATCTGGGTCGATCT
DN16955_c0_g1	LaPOD2	F: CATGGACTATGTCACGCCGA	R: GCTCGAAGAAAGCAATGGCA
DN18853_c0_g2	LaBAM1	F: CCCACGGCGAGAGAATTGTA	R: TTTGAGCGATGGGGACGTAG
	GADPH	F: TAGGAGGTGGCAGGACATCA	R: CCCTTTACCCGTCACGTTGT

Levels of chilling injury	Standard of judgment
1	Leaf present green and there are no symptoms of chilling injury
2	Only the edge of the leaf is yellow
3	Leaf withered and yellow part of < 50%, the green part is more
4	50%-100% of the leaf is dry and yellow, with less green
5	The whole plant is wilting
6	The aboveground parts dried up and died

Levels of chilling injury	Standard of judgment
1	Leaf present green and there are no symptoms of chilling injury
2	Only the edge of the leaf is yellow
3	Leaf withered and yellow part of < 50%, the green part is more
4	50%-100% of the leaf is dry and yellow, with less green
5	The whole plant is wilting
6	The aboveground parts dried up and died

Database	Number of annotated unigene	Percentage of annotated unigene (%)
NT	32797	42.73
NR	48554	63.26
Uniprot (BLASTX)	37801	49.25
Uniprot (BLASTP)	25631	33.39
PFAM	22340	29.11
eggNOG	28488	37.12
GO	24430	31.83
KEGG	15496	20.19
KOG	28787	37.51