Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (5): 631-636.DOI: 10.11983/CBB16156

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Simultaneous Determination of Jasmonic Acid and 12-Oxo- Phytodienoic Acid in Different Plant Materials by HPLC-MS/MS

Shuangyu Zhang1, Qi Qi1, Xiaohui Li2, Ying Gai1*, Xiangning Jiang1*   

  1. 1The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of Chinese Forestry Administration, College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
    2College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2016-07-27 Accepted:2016-12-05 Online:2017-09-01 Published:2017-07-10
  • Contact: Ying Gai, Xiangning Jiang

Abstract: Jasmonic acid is one of the important phytohormones responding to abiotic and biotic injury. Detecting the acid is difficult because of the low content in plant material. 12-oxo-phytodienoic acid is an important precursor in the biosynthesis of jasmonic acid. In this research, we established an HPLC-MS/MS method to determine the content of these two target chemicals simultaneously in 4 types of plant materials. An external standard curve was used to calculate the content in plant material. The regression coefficiency factor of the standard curve reached 0.991 9 to 0.999 5. Spiked recovery was from 91.91% to 101.56%. The method is sensitive and available; The limits of detection of two target chemicals were 3.218 and 34.129 pmol. This method was used to determinate the contents of these two target chemicals in 4 types of plant materials.

Key words: jasmonic acid, 12-oxo-phytodienoic acid, HPLC-MS/MS