Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (06): 728-734.DOI: 10.3969/j.issn.1674-3466.2009.06.011

• 技术方法 • Previous Articles     Next Articles

Transformation of Populus euphratica

Yuanwei Fan1, Aizhi Liu2, Huafang Wang1   

  1. 1National Engineering Laboratory for Tree Breeding, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University; 2Agriculture and Animal Husbandry Bureau of Alashan League of Inner Mongolia, Bayanhot
  • Received:2009-03-06 Revised:2009-09-03 Online:2009-11-01 Published:2009-11-01
  • Contact: Huafang Wang

Abstract: The uniquearbor tree species Populus euphratica can grow well in arid desert. We established an Agrobacterium based transformation system for this species using a GUS gene as the reporter. Shoots of P. euphratica were regenerated from leaves placed on 1/2 MS supplemented with 1.0 mmol.L-1 6-BA and 5.0 mmol.L-1 NAA, leaves were infected for 15 min in a solution of A. tumefaciens with 0.4-0.6 OD600 and 100 mmol.L-1 AS, and then co-cultured for 2 days. A. tumefaciens was inhibited with 300 mg.L-1 Cef, and transgenic tissues and shoots were selected with 9 mg.L-1 G418.