Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (3): 338-345.DOI: 10.3724/SP.J.1259.2015.00338

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Identification of Cold Tolerance at the Plumule Stage Quantitative Trait Loci with Single Segment Substituted Lines in Rice

Jinyan Zhu1, Mei Yang2, Chaoqiu Ji3, Jun Wang1, Jie Yang1, Fangjun Fan1, Wenqi Li1, Fangquan Wang1, Guohua Liang2, Yong Zhou2, *, Weigong Zhong1, *   

  1. 1Nanjing Branch of Chinese National Center for Rice Improvement, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
    2Jiangsu Provincial Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China
    3Jiangsu Oilfield Farm, Hongze 211706, China
  • Received:2014-05-26 Accepted:2014-09-29 Online:2015-05-01 Published:2015-04-08
  • Contact: Zhou Yong,Zhong Weigong
  • About author:

    ? These authors contributed equally to this paper

Abstract: Cold tolerance at the plumule stage is an important trait during rice growth and can be easily affected by genetic background and environmental factors. Single segment substitution lines (SSSLs) can effectively reduce the interference of the genetic background. We developed a set of genome-wide SSSLs carrying Nipponbare introgression segments in the background of Guangluai 4. Quantitative trait loci (QTL) analysis of cold tolerance at the plumule stage involved one-way ANOVA and Dunnett’s test for comparing SSSLs with the recurrent parent Guangluai 4 (P≤0.001). We detected eight QTL for cold tolerance at the plumule stage on chromosomes 1, 6, 8, 9 and 10; all had a positive effect. In both years studied, the additive effect of these QTL ranged from 14% to 44% and 10% to 45%, with additive effect percentages 700% to 2 200% and 500% to 2 250%. Four of these QTL were primarily mapped by using the substitution mapping method, and qCTP9-2 had the largest additive effect in both years for 44% and 45%, respectively. These results provide useful information for exploring and using new grain number-related resources.