Protocals for Oligonucleotide Fluorescence in situ Hybridization in Plants

doi:10.11983/CBB22265

Abstract

Abstract: Oligonucleotide fluorescence in situ hybridization (Oligo-FISH) is a new technology that integrates bioinformatics analysis, high throughput DNA synthesis and fluorescence in situ hybridization experiments. It is applicable to plant species with a reference genome. Oligo probes are able design to a chromosomal region, an entire chromosome, and a set of chromosomes according to researchers’ requirements. Oligo-FISH has been successfully applied to studies on karyotyping, chromosome variation, population evolution, homologous chromosome pairing, haplotype analysis, and heteropolyploid identification in several plant species. Here, we introduce the concrete operational processes of oligonucleotide-based probe design, amplification and labeling, chromosomes preparation, and fluorescence in situ hybridization in plants, so as to facilitate Oligo-FISH application in the cytogenetics research in the future.

Key words: fluorescence in situ hybridization, oligonucleotide probe, chromosome, plant

Fanfan Zhang, Xinying Xing, Wenqing Shi, Yi Shen, Zhukuan Cheng. Protocals for Oligonucleotide Fluorescence in situ Hybridization in Plants[J]. Chinese Bulletin of Botany, 2023, 58(2): 274-284.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22265

https://www.chinbullbotany.com/EN/Y2023/V58/I2/274

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