Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (6): 917-925.DOI: 10.11983/CBB22228

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

In vitro Culture of Taxus Rich in Taxanes

Xiaohui Feng1,2,3, Xuetong Yan2,3, Keyuan Zheng2,3, Qiang Zhou1, Weizhong Zhang4, Quanyong Wang4, Mulan Zhu2,3,*()   

  1. 1Key Laboratory of Plant Resources Conservation and Utilization, College of Hunan Province, Jishou University, Jishou 416000, China
    2Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China
    3Chinese Academic Scientific Center for Excellence in Molecular Plant Science, Shanghai 200032, China
    4Shanghai Jinhe Bio-Pharmaceutical Co., Ltd., Shanghai 201716, China
  • Received:2022-09-29 Accepted:2023-02-28 Online:2023-11-01 Published:2023-11-27
  • Contact: * E-mail: mlzhu@cemps.ac.cn

Abstract: Taxus contains the anti-cancer ingredient paclitaxel, is the top-protected endangered plant in China, and has great medicinal value but scarce resources. To solve the problem of paclitaxel source shortage, in this study, an in vitro culture of a taxane-rich Taxus system was established. The results showed that MS+2 mg·L-1 6-BA+0.4 mg·L-1 NAA+0.7 g·L-1 Pro was the optimal treatment for absorption surface expansion, and the expansion rate reached 90%. The adventitious bud high-frequency synchronous growth induction was in the DCR+1 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 Glu+1 g·L-1 AC. The best biomass amplification phase I was in MS+0.7 mg·L-1 6-BA+0.07 mg·L-1 NAA+0.1 mg·L-1 Phe+50 mg·L-1 PG, in which the biomass reached 542 mg. The optimized biomass amplification phase II was obtained in MS+0.5 mg·L-1 6-BA+0.05 mg·L-1 NAA+0.1 mg·L-1 Phe+2 g·L-1 AC, in which the biomass reached 1 612 mg. The content of taxanes in tissue culture materials is much higher than that of natural materials. The content of paclitaxel in tissue culture materials was 6.1 times that of natural materials. The contoent of baccatin III in tissue culture materials was 8.2 times that of natural materials. The content of 10-DAB in tissue culture materials was 68.1 times that of natural materials. This study established an in vitro culture system of taxane-rich Taxus, and solved the problem of paclitaxel drug shortage.

Key words: Taxus spp., absorbing surface, high-frequency synchronous growth, in vitro enrichment, taxanes