In vitro Culture of Taxus Rich in Taxanes

doi:10.11983/CBB22228

Abstract

Abstract: Taxus contains the anti-cancer ingredient paclitaxel, is the top-protected endangered plant in China, and has great medicinal value but scarce resources. To solve the problem of paclitaxel source shortage, in this study, an in vitro culture of a taxane-rich Taxus system was established. The results showed that MS+2 mg·L^-1 6-BA+0.4 mg·L^-1 NAA+0.7 g·L^-1 Pro was the optimal treatment for absorption surface expansion, and the expansion rate reached 90%. The adventitious bud high-frequency synchronous growth induction was in the DCR+1 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+5 mg·L^-1 Glu+1 g·L^-1 AC. The best biomass amplification phase I was in MS+0.7 mg·L^-1 6-BA+0.07 mg·L^-1 NAA+0.1 mg·L^-1 Phe+50 mg·L^-1 PG, in which the biomass reached 542 mg. The optimized biomass amplification phase II was obtained in MS+0.5 mg·L^-1 6-BA+0.05 mg·L^-1 NAA+0.1 mg·L^-1 Phe+2 g·L^-1 AC, in which the biomass reached 1 612 mg. The content of taxanes in tissue culture materials is much higher than that of natural materials. The content of paclitaxel in tissue culture materials was 6.1 times that of natural materials. The contoent of baccatin III in tissue culture materials was 8.2 times that of natural materials. The content of 10-DAB in tissue culture materials was 68.1 times that of natural materials. This study established an in vitro culture system of taxane-rich Taxus, and solved the problem of paclitaxel drug shortage.

Key words: Taxus spp., absorbing surface, high-frequency synchronous growth, in vitro enrichment, taxanes

Xiaohui Feng, Xuetong Yan, Keyuan Zheng, Qiang Zhou, Weizhong Zhang, Quanyong Wang, Mulan Zhu. In vitro Culture of Taxus Rich in Taxanes[J]. Chinese Bulletin of Botany, 2023, 58(6): 917-925.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22228

https://www.chinbullbotany.com/EN/Y2023/V58/I6/917

Figures/Tables 11

References 24

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Treatments	75% ethanol (s)	Disinfectant (min)	Pollution rate (%)	Survival rate (%)
1	10	3	68.9±5.09^a	31.1±5.09^f
2	10	5	35.6±3.85^c	57.8±3.85^bcd
3	10	7	20.0±3.33^de	62.2±3.85^bc
4	30	3	54.4±5.09^b	45.6±5.09^e
5	30	5	25.6±5.09^d	74.4±5.09^a
6	30	7	12.2±1.92^ef	64.4±8.38^b
7	50	3	41.1±5.09^c	51.1±1.92^de
8	50	5	16.7±3.33^e	64.4±5.09^b
9	50	7	5.6±1.92^f	53.3±5.77^de

Treatments	75% ethanol (s)	Disinfectant (min)	Pollution rate (%)	Survival rate (%)
1	10	3	68.9±5.09^a	31.1±5.09^f
2	10	5	35.6±3.85^c	57.8±3.85^bcd
3	10	7	20.0±3.33^de	62.2±3.85^bc
4	30	3	54.4±5.09^b	45.6±5.09^e
5	30	5	25.6±5.09^d	74.4±5.09^a
6	30	7	12.2±1.92^ef	64.4±8.38^b
7	50	3	41.1±5.09^c	51.1±1.92^de
8	50	5	16.7±3.33^e	64.4±5.09^b
9	50	7	5.6±1.92^f	53.3±5.77^de

Treatments	NAA (mg·L^-1)	6-BA (mg·L^-1)	Proline (g·L^-1)	Expansion rate (%)
1	0.1	1	0	28.9±5.09^g
2	0.1	2	0.1	53.3±8.82^ef
3	0.1	3	0.3	62.3±2.02^de
4	0.2	1	0.5	58.9±5.09^ef
5	0.2	2	0.7	76.7±6.67^abc
6	0.2	3	1	86.7±5.77^ab
7	0.3	1	0	47.8±6.93^f
8	0.3	2	0.1	51.1±15.40^ef
9	0.3	3	0.3	86.7±5.77^ab
10	0.4	1	0.5	55.6±5.09^ef
11	0.4	2	0.7	90.0±6.67^a
12	0.4	3	1	63.3±3.33^cde
13	0.5	1	0	52.2±1.92^ef
14	0.5	2	0.1	75.6±8.39^bcd
15	0.5	3	0.3	81.1±10.18^ab
16	0.6	1	0.5	64.4±11.71^cde
17	0.6	2	0.7	57.8±6.94^ef
18	0.6	3	1	46.7±5.77^f

Treatments	NAA (mg·L^-1)	6-BA (mg·L^-1)	Proline (g·L^-1)	Expansion rate (%)
1	0.1	1	0	28.9±5.09^g
2	0.1	2	0.1	53.3±8.82^ef
3	0.1	3	0.3	62.3±2.02^de
4	0.2	1	0.5	58.9±5.09^ef
5	0.2	2	0.7	76.7±6.67^abc
6	0.2	3	1	86.7±5.77^ab
7	0.3	1	0	47.8±6.93^f
8	0.3	2	0.1	51.1±15.40^ef
9	0.3	3	0.3	86.7±5.77^ab
10	0.4	1	0.5	55.6±5.09^ef
11	0.4	2	0.7	90.0±6.67^a
12	0.4	3	1	63.3±3.33^cde
13	0.5	1	0	52.2±1.92^ef
14	0.5	2	0.1	75.6±8.39^bcd
15	0.5	3	0.3	81.1±10.18^ab
16	0.6	1	0.5	64.4±11.71^cde
17	0.6	2	0.7	57.8±6.94^ef
18	0.6	3	1	46.7±5.77^f

Treatments	6-BA (mg·L^-1)	NAA (mg·L^-1)	Axillary buds induction rate (%)
1	0.5	0.05	63.3±3.33^ef
2	0.5	0.1	48.9±5.09^g
3	1	0.05	81.1±1.92^ab
4	1	0.1	88.9±5.09^a
5	1	0.2	68.9±5.09^cde
6	1	0.3	64.4±5.09^ef
7	2	0.05	73.3±3.34^bcd
8	2	0.1	75.6±3.85^bc
9	2	0.2	65.6±5.09^def
10	2	0.3	58.9±5.09^f
11	3	0.1	60.0±3.33^f
12	3	0.2	43.3±3.34^g