Pathogen Identification of Pea Crown Rot and Its Sensitivity to Fungicides

doi:10.11983/CBB22169

Abstract

Abstract:

Pea (Pisum sativum) is one of the most important economic crops of beans in China. The occurrence of diseases will cause great economic losses in pea production. Through morphological observation, molecular identification and pathogenicity determination, the results showed that the three kinds of pathogenic fungi causing pea crown rot were Fusarium oxysporum, Alternaria brassicae and F. grosmichelii, among which F. oxysporum was the most pathogenic, with the isolation frequency of 53.6%. The results of indoor toxicity measurement showed that five fungicides had inhibitory effects on the mycelial growth of three kinds of pathogenic bacteria, among which, the inhibition effect of fludioxonil and tebuconazole have top results. The results provide a scientific basis for the prevention and control of pea crown rot.

Key words: pea, crown rot, isolation and identification, fungicides, sensitivity determination

Yajing Song, Jinwen Ou, Guwen Zhang, Zhijuan Feng, Yuanpeng Bu, Bin Wang, Yaming Gong, Jianqiang Xu, Na Liu. Pathogen Identification of Pea Crown Rot and Its Sensitivity to Fungicides[J]. Chinese Bulletin of Botany, 2023, 58(1): 132-139.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22169

https://www.chinbullbotany.com/EN/Y2023/V58/I1/132

Figures/Tables 8

References 24

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Fungicides	Concentration 2 (μg?mL^-1)	Concentration 3 (μg?mL^-1)	Concentration 4 (μg?mL^-1)	Concentration 5 (μg?mL^-1)
Tebuconazole	0.4	0.6	0.8	1.0
Carbendazim	1.0	1.5	2.0	2.5
Fludioxonil	0.05	0.1	0.2	0.4
Iprodione	1.5	2.0	2.5	3.0
Kresoximmethyl	0.2	0.5	0.8	1.0

Fungicides	Concentration 2 (μg?mL^-1)	Concentration 3 (μg?mL^-1)	Concentration 4 (μg?mL^-1)	Concentration 5 (μg?mL^-1)
Tebuconazole	0.4	0.6	0.8	1.0
Carbendazim	1.0	1.5	2.0	2.5
Fludioxonil	0.05	0.1	0.2	0.4
Iprodione	1.5	2.0	2.5	3.0
Kresoximmethyl	0.2	0.5	0.8	1.0

Species	Isolates	Length of ITS (bp)	GenBank number	Species	Isolates	Length of ITS (bp)	GenBank number
Fusarium equiseti	BMS4	570	OM876892.1	A. solani	43	545	MN871610.1
F. falciforme	M232	530	MN907530.1	A. infectoria	F16	775	MW301214.1
F. solani	CFE-95	569	MW045606.1	Alternaria sp.	PYCC 8684	917	OK094892.1
F. oxysporum	S17F3	488	MK968009.1	Fusarium sp.	160	545	OM262322.1
F. grosmichelii	JXN4-10	514	OL744454.1	A. brassicae	W1 18S	589	JF439448.1
Alternaria brassicae	ALE-53	534	MF380676.1	F. nirenbergiae	9245	510	ON365710.1

Species	Isolates	Length of ITS (bp)	GenBank number	Species	Isolates	Length of ITS (bp)	GenBank number
Fusarium equiseti	BMS4	570	OM876892.1	A. solani	43	545	MN871610.1
F. falciforme	M232	530	MN907530.1	A. infectoria	F16	775	MW301214.1
F. solani	CFE-95	569	MW045606.1	Alternaria sp.	PYCC 8684	917	OK094892.1
F. oxysporum	S17F3	488	MK968009.1	Fusarium sp.	160	545	OM262322.1
F. grosmichelii	JXN4-10	514	OL744454.1	A. brassicae	W1 18S	589	JF439448.1
Alternaria brassicae	ALE-53	534	MF380676.1	F. nirenbergiae	9245	510	ON365710.1

Fungicides/Classification	Strain	Regression equation	EC₅₀ (μg?mL^-1)	Means±SD	r
Tebuconazole/Triazoles	YD-7	Y=0.8566X+5.1100	0.74	1.17±1.10	0.99
	YD-21	Y=2.0295X+4.1263	2.69		0.99
	YD-28	Y=0.7605X+5.8069	0.09		0.99
Carbendazim/Benzimidazoles	YD-7	Y=2.8425X+6.5769	0.28	8.28±10.96	0.97
	YD-21	Y=1.4663X+2.9820	23.78		0.99
	YD-28	Y=9.3029X+6.0223	0.78		0.96
Fludioxonil/Pyrroles	YD-7	Y=0.1054X+4.9459	3.26	1.12±1.52	0.99
	YD-21	Y=0.6536X+5.7413	0.07		0.99
	YD-28	Y=0.6242X+6.1209	0.02		0.99
Iprodione/Dicarbonamides	YD-7	Y=4.9338X+0.9405	6.65	3.44±2.46	0.97
	YD-21	Y=1.6099X+5.2905	0.66		0.99
	YD-28	Y=3.2891X+3.4191	3.02		0.98
Kresoximmethyl/Methoxy acrylates	YD-7	Y=0.9294X+4.9657	1.09	5.03±5.50	0.99
	YD-21	Y=1.0512X+3.8358	12.81		0.99
	YD-28	Y=1.0638X+4.9143	1.20		0.99