Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (1): 132-139.DOI: 10.11983/CBB22169
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Yajing Song1,2, Jinwen Ou2,3, Guwen Zhang2, Zhijuan Feng2, Yuanpeng Bu2, Bin Wang2, Yaming Gong2, Jianqiang Xu1,*(), Na Liu2,*(
)
Received:
2022-07-27
Accepted:
2022-11-14
Online:
2023-01-01
Published:
2023-01-05
Contact:
*E-mail: xujqhust@126.com;ln200811@163.com
Yajing Song, Jinwen Ou, Guwen Zhang, Zhijuan Feng, Yuanpeng Bu, Bin Wang, Yaming Gong, Jianqiang Xu, Na Liu. Pathogen Identification of Pea Crown Rot and Its Sensitivity to Fungicides[J]. Chinese Bulletin of Botany, 2023, 58(1): 132-139.
Fungicides | Concentration 1 (μg?mL-1) | Concentration 2 (μg?mL-1) | Concentration 3 (μg?mL-1) | Concentration 4 (μg?mL-1) | Concentration 5 (μg?mL-1) |
---|---|---|---|---|---|
Tebuconazole | 0 | 0.4 | 0.6 | 0.8 | 1.0 |
Carbendazim | 0 | 1.0 | 1.5 | 2.0 | 2.5 |
Fludioxonil | 0 | 0.05 | 0.1 | 0.2 | 0.4 |
Iprodione | 0 | 1.5 | 2.0 | 2.5 | 3.0 |
Kresoximmethyl | 0 | 0.2 | 0.5 | 0.8 | 1.0 |
Table 1 Concentration settings of 5 fungicides
Fungicides | Concentration 1 (μg?mL-1) | Concentration 2 (μg?mL-1) | Concentration 3 (μg?mL-1) | Concentration 4 (μg?mL-1) | Concentration 5 (μg?mL-1) |
---|---|---|---|---|---|
Tebuconazole | 0 | 0.4 | 0.6 | 0.8 | 1.0 |
Carbendazim | 0 | 1.0 | 1.5 | 2.0 | 2.5 |
Fludioxonil | 0 | 0.05 | 0.1 | 0.2 | 0.4 |
Iprodione | 0 | 1.5 | 2.0 | 2.5 | 3.0 |
Kresoximmethyl | 0 | 0.2 | 0.5 | 0.8 | 1.0 |
Figure 2 Morphological characteristics of pathogenic fungus YD-7, YD-21 and YD-28 of pea crown rot (A1)-(A3) Colony and spore morphology of YD-7; (B1)-(B3) Colony and spore morphology of YD-21; (C1)-(C3) Colony and spore morphology of YD-28. (A1), (A2), (B1), (B2), (C1), (C2) Bars=2 cm; (A3), (B3), (C3) Bars=25 μm
Figure 3 Agarose gel electrophoresis of PCR products of pea crown rot pathogen and phylogenetic tree of related sibling species (A) Agarose gel electrophoresis of PCR products of isolated strains YD-7, YD-21 and YD-28; (B) Phylogenetic tree based on ITS and its homologous sequences
Species | Isolates | Length of ITS (bp) | GenBank number | Species | Isolates | Length of ITS (bp) | GenBank number |
---|---|---|---|---|---|---|---|
Fusarium equiseti | BMS4 | 570 | OM876892.1 | A. solani | 43 | 545 | MN871610.1 |
F. falciforme | M232 | 530 | MN907530.1 | A. infectoria | F16 | 775 | MW301214.1 |
F. solani | CFE-95 | 569 | MW045606.1 | Alternaria sp. | PYCC 8684 | 917 | OK094892.1 |
F. oxysporum | S17F3 | 488 | MK968009.1 | Fusarium sp. | 160 | 545 | OM262322.1 |
F. grosmichelii | JXN4-10 | 514 | OL744454.1 | A. brassicae | W1 18S | 589 | JF439448.1 |
Alternaria brassicae | ALE-53 | 534 | MF380676.1 | F. nirenbergiae | 9245 | 510 | ON365710.1 |
Table 2 ITS sequence length of the strains
Species | Isolates | Length of ITS (bp) | GenBank number | Species | Isolates | Length of ITS (bp) | GenBank number |
---|---|---|---|---|---|---|---|
Fusarium equiseti | BMS4 | 570 | OM876892.1 | A. solani | 43 | 545 | MN871610.1 |
F. falciforme | M232 | 530 | MN907530.1 | A. infectoria | F16 | 775 | MW301214.1 |
F. solani | CFE-95 | 569 | MW045606.1 | Alternaria sp. | PYCC 8684 | 917 | OK094892.1 |
F. oxysporum | S17F3 | 488 | MK968009.1 | Fusarium sp. | 160 | 545 | OM262322.1 |
F. grosmichelii | JXN4-10 | 514 | OL744454.1 | A. brassicae | W1 18S | 589 | JF439448.1 |
Alternaria brassicae | ALE-53 | 534 | MF380676.1 | F. nirenbergiae | 9245 | 510 | ON365710.1 |
Figure 5 Pathogenicity of isolated strains YD-7, YD-21 and YD-28 to Zhewan 2 (A) Inoculate PDA as control; (B) Pathogenicity of YD-7 to Zhewan 2; (C) Pathogenicity of YD-21 to Zhewan 2; (D) Pathogenicity of YD-28 to Zhewan 2. Bars=5 cm
Fungicides/Classification | Strain | Regression equation | EC50 (μg?mL-1) | Means±SD | r |
---|---|---|---|---|---|
Tebuconazole/Triazoles | YD-7 | Y=0.8566X+5.1100 | 0.74 | 1.17±1.10 | 0.99 |
YD-21 | Y=2.0295X+4.1263 | 2.69 | 0.99 | ||
YD-28 | Y=0.7605X+5.8069 | 0.09 | 0.99 | ||
Carbendazim/Benzimidazoles | YD-7 | Y=2.8425X+6.5769 | 0.28 | 8.28±10.96 | 0.97 |
YD-21 | Y=1.4663X+2.9820 | 23.78 | 0.99 | ||
YD-28 | Y=9.3029X+6.0223 | 0.78 | 0.96 | ||
Fludioxonil/Pyrroles | YD-7 | Y=0.1054X+4.9459 | 3.26 | 1.12±1.52 | 0.99 |
YD-21 | Y=0.6536X+5.7413 | 0.07 | 0.99 | ||
YD-28 | Y=0.6242X+6.1209 | 0.02 | 0.99 | ||
Iprodione/Dicarbonamides | YD-7 | Y=4.9338X+0.9405 | 6.65 | 3.44±2.46 | 0.97 |
YD-21 | Y=1.6099X+5.2905 | 0.66 | 0.99 | ||
YD-28 | Y=3.2891X+3.4191 | 3.02 | 0.98 | ||
Kresoximmethyl/Methoxy acrylates | YD-7 | Y=0.9294X+4.9657 | 1.09 | 5.03±5.50 | 0.99 |
YD-21 | Y=1.0512X+3.8358 | 12.81 | 0.99 | ||
YD-28 | Y=1.0638X+4.9143 | 1.20 | 0.99 |
Table 3 Toxicity measurement of different fungicides to YD-7, YD-21 and YD-28
Fungicides/Classification | Strain | Regression equation | EC50 (μg?mL-1) | Means±SD | r |
---|---|---|---|---|---|
Tebuconazole/Triazoles | YD-7 | Y=0.8566X+5.1100 | 0.74 | 1.17±1.10 | 0.99 |
YD-21 | Y=2.0295X+4.1263 | 2.69 | 0.99 | ||
YD-28 | Y=0.7605X+5.8069 | 0.09 | 0.99 | ||
Carbendazim/Benzimidazoles | YD-7 | Y=2.8425X+6.5769 | 0.28 | 8.28±10.96 | 0.97 |
YD-21 | Y=1.4663X+2.9820 | 23.78 | 0.99 | ||
YD-28 | Y=9.3029X+6.0223 | 0.78 | 0.96 | ||
Fludioxonil/Pyrroles | YD-7 | Y=0.1054X+4.9459 | 3.26 | 1.12±1.52 | 0.99 |
YD-21 | Y=0.6536X+5.7413 | 0.07 | 0.99 | ||
YD-28 | Y=0.6242X+6.1209 | 0.02 | 0.99 | ||
Iprodione/Dicarbonamides | YD-7 | Y=4.9338X+0.9405 | 6.65 | 3.44±2.46 | 0.97 |
YD-21 | Y=1.6099X+5.2905 | 0.66 | 0.99 | ||
YD-28 | Y=3.2891X+3.4191 | 3.02 | 0.98 | ||
Kresoximmethyl/Methoxy acrylates | YD-7 | Y=0.9294X+4.9657 | 1.09 | 5.03±5.50 | 0.99 |
YD-21 | Y=1.0512X+3.8358 | 12.81 | 0.99 | ||
YD-28 | Y=1.0638X+4.9143 | 1.20 | 0.99 |
[1] | 陈星, 高淑梅, 李迎宾, 张治萍, 蒋娜, 罗来鑫, 李健强 (2017). 广西防城港西番莲茎基腐病发生为害调查与病原鉴定. 见:彭友良, 李向东主编. 中国植物病理学会2017年学术年会论文集. 北京: 中国农业科学技术出版社. pp. 168. |
[2] | 崔翠, 孙建蓉, 赵愉风, 郜欢欢, 程闯, 王瑞莉, 王刘艳, 周清元 (2019). 豌豆嫩尖几个营养品质性状的遗传多样性分析及其综合评价. 植物遗传资源学报 20, 932-948. |
[3] | 段建华, 袁同高 (2018). 玉米茎基腐病的发生规律及防治措施. 吉林农业 (4), 69. |
[4] | 范青, 田世平, 李永兴, 徐勇, 汪沂 (2000). 枯草芽孢杆菌(B-912) 对桃和油桃褐腐病的抑制效果. 植物学报 42, 1137-1143. |
[5] | 黄端, 夏宏秋, 吴齐仟 (2015). 食荚豌豆死苗成因及防治初探. 中国农业信息 27(17), 44. |
[6] | 纪莉景, 栗秋生, 王亚娇, 吴玉星, 李聪聪, 孙梦伟, 孔令晓 (2020). 温度对假禾谷镰刀菌生长、侵染及茎基腐病发生的影响. 植物病理学报 50, 723-730. |
[7] | 连荣芳, 王梅春, 墨金萍, 肖贵 (2013). 生防菌剂浸种对豌豆苗期茎基腐病的田间防效. 甘肃农业科技 44(3), 10-11. |
[8] | 林汝法, 柴岩, 廖琴, 孙世贤 (2002). 中国小杂粮. 北京: 中国农业科学技术出版社. pp. 317-319. |
[9] | 栾冬冬, 贾吉玉, 王光州, 张俊伶 (2022). 中国小麦茎基腐病的发生现状及防治策略. 麦类作物学报 42, 512-520. |
[10] | 马传禹, 姚丽姗, 杜腓利, 徐明良 (2018). 玉米抗茎腐病研究进展. 玉米科学 26(2), 131-137. |
[11] |
漆永红, 李敏权, 曹素芳, 蒋晶晶, 李雪萍, 李青青, 徐美蓉, 李继平 (2021). 党参茎基腐病镰孢菌鉴定及其对杀菌剂的敏感性研究. 农学学报 11(2), 74-78.
DOI |
[12] | 邱金忠 (2015). 百香果茎基腐病的发生与防治方法. 中国农业信息 27(1), 50. |
[13] | 沈广爽, 于淑晶, 郭宁, 周贤龙, 边强 (2021). 玉米茎基腐病防治研究进展. 农药 60, 235-238. |
[14] | 万述伟, 宋凤景, 郝俊杰, 张晓艳, 李红卫, 邵阳, 赵爱鸿 (2017). 271份豌豆种质资源农艺性状遗传多样性分析. 植物遗传资源学报 18, 10-18. |
[15] |
王飞, 杨瑾, 李雪梦, 高素霞, 文艺, 刘玉霞, 秦艳红, 戚文平, 鲁传涛 (2022). 丹参茎基腐病病原鉴定及防治药剂筛选. 植物病理学报 doi: 10.13926/j.cnki.apps.000779.
DOI |
[16] | 王广印, 郭卫丽, 陈碧华, 计善玲, 薛晓庆 (2018). 大棚秋番茄茎基腐病防控技术研究. 见: 中国园艺学会2018年学术年会论文摘要集. 青岛: 中国园艺学会. pp. 163. |
[17] | 王莉莉, 徐建强, 朱凯, 孙莹, 郑伟, 侯颖 (2022). 醚菌酯对假禾谷镰孢的抑制作用及对小麦茎基腐病的防效研究. 植物病理学报 52, 434-442. |
[18] | 徐飞, 韩自行, 宋玉立, 刘伟, 周益林, 李丽娟, 王俊美, 秦志英, 程燕君 (2022). 几种杀菌剂对小麦茎基腐病的防治效果. 植物保护 48(2), 296-302. |
[19] | 郑卓杰 (1997). 中国食用豆类学. 北京: 中国农业出版社. pp. 88-127. |
[20] | 宗绪晓 (2016). 豌豆生产技术. 北京: 北京教育出版社. pp. 1-10. |
[21] |
Chakraborty S, Obanor F, Westecott R, Abeywickrama K (2010). Wheat crown rot pathogens Fusarium graminearum and F. pseudograminearum lack specialization. Phytopathology 100, 1057-1065.
DOI PMID |
[22] |
Hogg AC, Johnston RH, Dyer AT (2007). Applying real-time quantitative PCR to Fusarium crown rot of wheat. Plant Dis 91, 1021-1028.
DOI PMID |
[23] |
Kazan K, Gardiner DM (2018). Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects. Mol Plant Pathol 19, 1547-1562.
DOI URL |
[24] |
Strausbaugh CA, Bradley CA, Koehn AC, Forster RL (2004). Survey of root diseases of wheat and barley in southeastern Idaho. Can J Plant Pathol 26, 167-176.
DOI URL |
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