Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (4): 656-667.DOI: 10.11983/CBB22160

• SPECIAL TOPICS • Previous Articles    

Research Progress on Catalytic Characteristics of Pinoresinol-lariciresinol Reductase in Plants

Yanhong Yin1, Wansheng Chen1,2(), Ying Xiao1()   

  1. 1. Research and Development Center of Chinese Medicine Resources and Biotechnology, The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Department of Pharmacy, Changzheng Hospital, Naval Medical University, Shanghai 200003, China

Abstract: Pinoresinol-lariciresinol reductases (PLRs) are key enzymes involved in the lignan biosynthesis in plants, which convert pinoresinol to lariciresinol and then to secoisolariciresinol. PLRs are NADPH-dependent reductases with substrate stereoselectivity. The catalytic products of PLR are the sources of different types of 8-8′ lignans, and the substrate selectivity directly determines the skeleton types of lignans, such as furano, dibenzylbutane, dibenzylbutyrolactone and aryltetrahydronaphthalene lignans. Therefore, the catalytic and expression characteristics of PLRs play an important role in the composition and biodiversity of lignans in plants. This paper reviewed the research progress on the important role of PLRs in lignans biosynthesis, as well as its enantioselectivity and catalytic mechanism, thus to lay the foundation for further study on the biological function and catalytic mechanism of PLR genes, and point out the direction for the precise biosynthesis of different types of lignans enantiomers through synthetic biology.

Key words: pinoresinol-lariciresinol reductases (PLRs), lignans, catalytic characteristics, enantioselectivity, catalytic mechanism