Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (1): 33-43.DOI: 10.11983/CBB20147

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Genome-wide Identification and Analysis of CONSTANS-like Gene Family in Nicotiana tabacum

Yawen Zhang1,2, Shan Liang1, Guoyun Xu3, Wuxia Guo2,4,*(), Shulin Deng2,4,*()   

  1. 1Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    2Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
    3Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou 450001, China
    4Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
  • Received:2020-08-25 Accepted:2021-01-05 Online:2021-01-01 Published:2021-01-15
  • Contact: Wuxia Guo,Shulin Deng

Abstract: Nicotiana tabacum (tobacco) is one of the model plants for molecular biology research as well as an important economic crop in the world. A suitable living environment is essential for the growth and reproduction of tobacco. CONSTANS-like (COL) family proteins are not only key regulators for flowering time, but also play important roles in stress response of plants. Our aim was to identify the COL family members, analyze their gene structure, evolutionary relationship, transcriptional regulatory elements and expression patterns, and explore their possible functions in response to cold stress in tobacco. We identified a total of 15 COL genes with similar physiochemical properties in tobacco. Evolutionary analysis divided all COL genes into three categories, and similar intron structure and motif distribution were observed among genes within each category. The promoter regions of tobacco COL genes contain a large number of cis-acting elements related to responses to light, low temperature, drought and phyto hormone. Gene expression analysis showed that low temperature significantly affected the expression of COL genes in tobacco, but the effects on different genes were different. Our study showed different parental (N. sylvestris (maternal) and N. tomentosiformis (paternal)) expression bias between different COL genes in tobacco, and most of the bias patterns were maintained from 6-7 leaf stage to budding stage.

Key words: Nicotiana tabacum, COL, phylogeny, low temperature, expression bias