Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana

doi:10.11983/CBB19127

Abstract

Abstract:

Meiosis halves the number of chromosomes to produce haploid gametes through nuclear division twice following single round of DNA replication, and is essential for eukaryotic sexual reproduction. Arabidopsis thaliana is a traditional model organism used for molecular genetic study. Recently, with breakthroughs in microscopic technologies for observing chromosomes, analyses of chromosome morphology during meiosis using cytological methods have made great advances to understand molecular and genetic mechanisms in regulation of meiosis. In this study, we described in detail the chromosome spread technique we developed, which is well used for observation of chromosome behaviors in Arabidopsis male meiocytes.

Key words: Arabidopsis thaliana, meiocytes, chromosome spread, FISH

Wanyue Xu,Yingxiang Wang. Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana[J]. Chinese Bulletin of Botany, 2019, 54(5): 620-624.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB19127

https://www.chinbullbotany.com/EN/Y2019/V54/I5/620

1	Ma H (2006). A molecular portrait of Arabidopsis meiosis. Arabidopsis Book 4, e0095.
2	Sanchez-Moran E, Armstrong SJ (2014). Meiotic chromosome synapsis and recombination in Arabidopsis thaliana: new ways of integrating cytological and mo-lecular approaches. Chromosome Res 22, 179-190.
3	Sanders PM, Bui AQ, Weterings K, McIntire KN, Hsu YC, Lee PY, Truong MT, Beals TP, Goldberg RB (1999). Anther developmental defects in Arabidopsis thaliana male-sterile mutants. Sex Plant Reprod 11, 297-322.
4	The Arabidopsis Genome Initiative (2000). Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796-815.
5	Wang YX, Cheng ZH, Lu PL, Timofejeva L, Ma H (2014). Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana. In: Riechmann JL, Wellmer F, eds. Flower Development: Methods and Protocols. New York: Humana Press. pp. 217-230.
6	Wang YX, Copenhaver GP (2018). Meiotic recombination: mixing it up in plants. Annu Rev Plant Biol 69, 577-609.

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Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana

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