Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (2): 194-207.DOI: 10.11983/CBB18184

Special Issue: 逆境生物学专辑 (2019年54卷2期)

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Effect of Iron Deficiency on the Protein Profile of Rice (Oryza sativa) Phloem Sap

Lin Chen,Yan Lin,Pengfei Chen,Shaohua Wang,Yanfeng Ding()   

  1. College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2018-08-28 Accepted:2019-01-11 Online:2019-03-01 Published:2019-09-01
  • Contact: Yanfeng Ding

Abstract: In order to identify long-distance signals under iron (Fe) deficiency in rice (Oryza sativa), TMT label technique was used to study the protein profile of phloem sap under different iron concentrations. A total of 206 differentially ex- pressed proteins were identified: 54 were upregulated and 152 were downregulated. Most of these proteins are involved in hormone signal transduction, carbon metabolism, glutathione metabolism and mRNA transport. In addition, we mea- sured the physiological indicators according to the differentially expressed proteins. It was found that phytohormones, sucrose, glutathione and transporters were significantly changed under iron deficiency. To further study the function of these proteins can help to reveal the long distance signaling pathway of rice in response to Fe deficiency.

Key words: rice (Oryza sativa), iron deficiency, phloem, long-distance signal, TMT